Advanced search
2 files | 1.56 MB

Distinct neutrophil C5a receptor inflammatory events in cows initiated by chemoattractant C5a and lipopolysaccharide around parturition and in mid lactation

Xanthippe Boulougouris (UGent) , Carolien Rogiers (UGent) , Mario Van Poucke (UGent) , Bart De Spiegeleer (UGent) , Luc Peelman (UGent) , Luc Duchateau (UGent) and Christian Burvenich (UGent)
(2019) JOURNAL OF DAIRY SCIENCE. 102(2). p.1457-1472
Author
Organization
Abstract
In neutrophils, toll-like receptor and complement component 5a (C5a) signaling are critical pathways regulating innate immunity. In cows, not much is known about the second C5a receptor, complement component 5a receptor 2 (C5AR2). It is an interesting player in sepsis treatment because it is considered to have an anti-inflammatory effect during normal inflammation. Periparturient cows are prone to severe infections, and the objectives of this study were to investigate the expression and functionality of C5AR2 during peripartum. We investigated the effect of 2 major inflammatory stimuli, C5a and lipopolysaccharide (LPS), on the expression of a selected number of genes (C5AR1, C5AR2, TLR4 ITGAM, COX2, and CXCL8) and functions linked to these receptors. Overall, TLR4, ITGAM, and C5AR2, all of which are involved in early inflammation, showed a lower expression in periparturient cows. However, an overall lower expression seems not to be the only explanation for the increased risk of sepsis in periparturient cows. Normally, in response to inflammation and as seen in the mid-lactation group, the expression of these genes increases after stimulation with LPS. However, in periparturient cows, stimulation with LPS led to a decrease in expression of these receptors, indicating a different response of neutrophils in response to LPS during this period. A decrease in ITGAM (coding for CD11b) expression complicates correct neutrophil localization and phagocytosis. Its downregulation upon stimulation might be detrimental for adequate eradication of the pathogen and might increase the risk of an imbalanced inflammation; C5AR2 seems to play a central role in this altered response. In addition, myeloperoxidase (MPO) activity in periparturient cows is lower in response to C5a stimulation. It has been suggested that MPO plays an important role in neutrophil shutdown and, thereby, timely resolution of inflammation. A decreased MPO activity might thus prolong the inflammatory reaction of the neutrophils. This finding was supported by the increased viability of the neutrophils obtained from periparturient cows. Even after stimulation, we found a lower caspase-3 activity in this group, indicating that they might be activated for a longer time compared with the neutrophils from mid-lactation cows. Accordingly, these alterations might contribute to a temporal mismatch in inflammatory responses, as often seen in severe periparturient infections.
Keywords
peripartum, neutrophil, C5AR2, CD11b, ESCHERICHIA-COLI MASTITIS, COMPLEMENT FRAGMENT C5A, BOVINE NEUTROPHIL, OXIDATIVE BURST, DAIRY-COWS, POLYMORPHONUCLEAR LEUKOCYTES, GENE-EXPRESSION, CELL-VOLUME, L-SELECTIN, BLOOD

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 810.96 KB
  • (...).ppt
    • supplementary material
    • |
    • UGent only
    • |
    • PowerPoint
    • |
    • 747.01 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Boulougouris, Xanthippe, Carolien Rogiers, Mario Van Poucke, Bart De Spiegeleer, Luc Peelman, Luc Duchateau, and Christian Burvenich. 2019. “Distinct Neutrophil C5a Receptor Inflammatory Events in Cows Initiated by Chemoattractant C5a and Lipopolysaccharide Around Parturition and in Mid Lactation.” Journal of Dairy Science 102 (2): 1457–1472.
APA
Boulougouris, X., Rogiers, C., Van Poucke, M., De Spiegeleer, B., Peelman, L., Duchateau, L., & Burvenich, C. (2019). Distinct neutrophil C5a receptor inflammatory events in cows initiated by chemoattractant C5a and lipopolysaccharide around parturition and in mid lactation. JOURNAL OF DAIRY SCIENCE, 102(2), 1457–1472.
Vancouver
1.
Boulougouris X, Rogiers C, Van Poucke M, De Spiegeleer B, Peelman L, Duchateau L, et al. Distinct neutrophil C5a receptor inflammatory events in cows initiated by chemoattractant C5a and lipopolysaccharide around parturition and in mid lactation. JOURNAL OF DAIRY SCIENCE. 2019;102(2):1457–72.
MLA
Boulougouris, Xanthippe et al. “Distinct Neutrophil C5a Receptor Inflammatory Events in Cows Initiated by Chemoattractant C5a and Lipopolysaccharide Around Parturition and in Mid Lactation.” JOURNAL OF DAIRY SCIENCE 102.2 (2019): 1457–1472. Print.
@article{8610979,
  abstract     = {In neutrophils, toll-like receptor and complement component 5a (C5a) signaling are critical pathways regulating innate immunity. In cows, not much is known about the second C5a receptor, complement component 5a receptor 2 (C5AR2). It is an interesting player in sepsis treatment because it is considered to have an anti-inflammatory effect during normal inflammation. Periparturient cows are prone to severe infections, and the objectives of this study were to investigate the expression and functionality of C5AR2 during peripartum. We investigated the effect of 2 major inflammatory stimuli, C5a and lipopolysaccharide (LPS), on the expression of a selected number of genes (C5AR1, C5AR2, TLR4 ITGAM, COX2, and CXCL8) and functions linked to these receptors. Overall, TLR4, ITGAM, and C5AR2, all of which are involved in early inflammation, showed a lower expression in periparturient cows. However, an overall lower expression seems not to be the only explanation for the increased risk of sepsis in periparturient cows. Normally, in response to inflammation and as seen in the mid-lactation group, the expression of these genes increases after stimulation with LPS. However, in periparturient cows, stimulation with LPS led to a decrease in expression of these receptors, indicating a different response of neutrophils in response to LPS during this period. A decrease in ITGAM (coding for CD11b) expression complicates correct neutrophil localization and phagocytosis. Its downregulation upon stimulation might be detrimental for adequate eradication of the pathogen and might increase the risk of an imbalanced inflammation; C5AR2 seems to play a central role in this altered response. In addition, myeloperoxidase (MPO) activity in periparturient cows is lower in response to C5a stimulation. It has been suggested that MPO plays an important role in neutrophil shutdown and, thereby, timely resolution of inflammation. A decreased MPO activity might thus prolong the inflammatory reaction of the neutrophils. This finding was supported by the increased viability of the neutrophils obtained from periparturient cows. Even after stimulation, we found a lower caspase-3 activity in this group, indicating that they might be activated for a longer time compared with the neutrophils from mid-lactation cows. Accordingly, these alterations might contribute to a temporal mismatch in inflammatory responses, as often seen in severe periparturient infections.},
  author       = {Boulougouris, Xanthippe and Rogiers, Carolien and Van Poucke, Mario and De Spiegeleer, Bart and Peelman, Luc and Duchateau, Luc and Burvenich, Christian},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keywords     = {peripartum,neutrophil,C5AR2,CD11b,ESCHERICHIA-COLI MASTITIS,COMPLEMENT FRAGMENT C5A,BOVINE NEUTROPHIL,OXIDATIVE BURST,DAIRY-COWS,POLYMORPHONUCLEAR LEUKOCYTES,GENE-EXPRESSION,CELL-VOLUME,L-SELECTIN,BLOOD},
  language     = {eng},
  number       = {2},
  pages        = {1457--1472},
  title        = {Distinct neutrophil C5a receptor inflammatory events in cows initiated by chemoattractant C5a and lipopolysaccharide around parturition and in mid lactation},
  url          = {http://dx.doi.org/10.3168/jds.2018-15356},
  volume       = {102},
  year         = {2019},
}

Altmetric
View in Altmetric
Web of Science
Times cited: