Screening of two-photon activated photodynamic therapy sensitizers using a 3D osteosarcoma model
- Author
- Agnes Dobos, Wolfgang Steiger, Dominik Theiner, Peter Gruber, Markus Lunzer, Jasper Van Hoorick (UGent) , Sandra Van Vlierberghe (UGent) and Aleksandr Ovsianikov
- Organization
- Abstract
- Photodynamic therapy (PDT) involves a photosensitizing agent activated with light to induce cell death. Two-photon excited PDT (TPE-PDT) offers numerous benefits compared to traditional one-photon induced PDT, including an increased penetration depth and precision. However, the in vitro profiling and comparison of two-photon photosensitizers (PS) are still troublesome. Herein, we report the development of an in vitro screening platform of TPE-PS using a 3D osteosarcoma cell culture. The platform was tested using three different two-photon (2P) active compounds - a 2P sensitizer P2CK, a fluorescent dye Eosin Y, and a porphyrin derivative (TPP). Their 2P absorption cross-sections (sigma(2PA)) were characterised using a fully automated z-scan setup. TPP exhibited a remarkably high sigma(2PA) at 720 nm (8865 GM) and P2CK presented a high absorption at 850 nm (405 GM), while Eosin Y had the lowest 2P absorption at the studied wavelengths (<100 GM). The cellular uptake of PS visualized using confocal laser scanning microscopy showed that both TPP and P2CK were internalized by the cells, while Eosin Y stayed mainly in the surrounding media. The efficiency of the former two TPE-PS was quantified using the PrestoBlue metabolic assay, showing a significant reduction in cell viability after two-photon irradiation. The possibility of damage localization was demonstrated using a co-culture of adipose derived stem cells together with osteosarcoma spheroids showing no signs of damage to the surrounding healthy cells after TPE-PDT.
- Keywords
- SINGLET OXYGEN, PHOTOSENSITIZERS, EXCITATION, CELLS, PENETRATION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8610535
- MLA
- Dobos, Agnes, et al. “Screening of Two-Photon Activated Photodynamic Therapy Sensitizers Using a 3D Osteosarcoma Model.” ANALYST, vol. 144, no. 9, 2019, pp. 3056–63, doi:10.1039/c9an00068b.
- APA
- Dobos, A., Steiger, W., Theiner, D., Gruber, P., Lunzer, M., Van Hoorick, J., … Ovsianikov, A. (2019). Screening of two-photon activated photodynamic therapy sensitizers using a 3D osteosarcoma model. ANALYST, 144(9), 3056–3063. https://doi.org/10.1039/c9an00068b
- Chicago author-date
- Dobos, Agnes, Wolfgang Steiger, Dominik Theiner, Peter Gruber, Markus Lunzer, Jasper Van Hoorick, Sandra Van Vlierberghe, and Aleksandr Ovsianikov. 2019. “Screening of Two-Photon Activated Photodynamic Therapy Sensitizers Using a 3D Osteosarcoma Model.” ANALYST 144 (9): 3056–63. https://doi.org/10.1039/c9an00068b.
- Chicago author-date (all authors)
- Dobos, Agnes, Wolfgang Steiger, Dominik Theiner, Peter Gruber, Markus Lunzer, Jasper Van Hoorick, Sandra Van Vlierberghe, and Aleksandr Ovsianikov. 2019. “Screening of Two-Photon Activated Photodynamic Therapy Sensitizers Using a 3D Osteosarcoma Model.” ANALYST 144 (9): 3056–3063. doi:10.1039/c9an00068b.
- Vancouver
- 1.Dobos A, Steiger W, Theiner D, Gruber P, Lunzer M, Van Hoorick J, et al. Screening of two-photon activated photodynamic therapy sensitizers using a 3D osteosarcoma model. ANALYST. 2019;144(9):3056–63.
- IEEE
- [1]A. Dobos et al., “Screening of two-photon activated photodynamic therapy sensitizers using a 3D osteosarcoma model,” ANALYST, vol. 144, no. 9, pp. 3056–3063, 2019.
@article{8610535,
abstract = {{Photodynamic therapy (PDT) involves a photosensitizing agent activated with light to induce cell death. Two-photon excited PDT (TPE-PDT) offers numerous benefits compared to traditional one-photon induced PDT, including an increased penetration depth and precision. However, the in vitro profiling and comparison of two-photon photosensitizers (PS) are still troublesome. Herein, we report the development of an in vitro screening platform of TPE-PS using a 3D osteosarcoma cell culture. The platform was tested using three different two-photon (2P) active compounds - a 2P sensitizer P2CK, a fluorescent dye Eosin Y, and a porphyrin derivative (TPP). Their 2P absorption cross-sections (sigma(2PA)) were characterised using a fully automated z-scan setup. TPP exhibited a remarkably high sigma(2PA) at 720 nm (8865 GM) and P2CK presented a high absorption at 850 nm (405 GM), while Eosin Y had the lowest 2P absorption at the studied wavelengths (<100 GM). The cellular uptake of PS visualized using confocal laser scanning microscopy showed that both TPP and P2CK were internalized by the cells, while Eosin Y stayed mainly in the surrounding media. The efficiency of the former two TPE-PS was quantified using the PrestoBlue metabolic assay, showing a significant reduction in cell viability after two-photon irradiation. The possibility of damage localization was demonstrated using a co-culture of adipose derived stem cells together with osteosarcoma spheroids showing no signs of damage to the surrounding healthy cells after TPE-PDT.}},
author = {{Dobos, Agnes and Steiger, Wolfgang and Theiner, Dominik and Gruber, Peter and Lunzer, Markus and Van Hoorick, Jasper and Van Vlierberghe, Sandra and Ovsianikov, Aleksandr}},
issn = {{0003-2654}},
journal = {{ANALYST}},
keywords = {{SINGLET OXYGEN,PHOTOSENSITIZERS,EXCITATION,CELLS,PENETRATION}},
language = {{eng}},
number = {{9}},
pages = {{3056--3063}},
title = {{Screening of two-photon activated photodynamic therapy sensitizers using a 3D osteosarcoma model}},
url = {{http://doi.org/10.1039/c9an00068b}},
volume = {{144}},
year = {{2019}},
}
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