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Impact of cross-contamination concentrations of doxycycline hyclate on the microbial ecosystem in an ex vivo model of the pig's cecum

(2019) MICROBIAL DRUG RESISTANCE. 25(2). p.304-315
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Abstract
Aims: Cross-contamination of feed with antibiotics causes pigs to become unintentionally exposed to low concentrations of antibiotics. This study investigates the effect of residues of doxycycline hyclate (DOX) in an ex vivo model of the intestinal tract of pigs, focusing on the microbial community, microbial activity, and the enrichment of resistant bacteria and resistance genes. Results: The effect of three concentrations DOX were tested; 1 and 4mg/L correspond to the intestinal concentrations when pigs are fed a compound feed containing 3% of a therapeutic dose, and a reference concentration of 16mg/L. These were continuously administered to a chemostat, simulating the microbial ecosystem of the pig cecum and inoculated with cecal content of organically grown pigs. The administration of even the lowest DOX concentration caused a significant decrease in bacterial activity, while the microbial community profile appeared to remain unaffected by any of the concentrations. A concentration of 1mg/L DOX caused minor selection pressure for tetracycline-resistant Escherichia coli but no other groups enumerated with plate cultivation, while 4mg/L induced major enrichment of tetracycline-resistant E. coli, Enterobacteriaceae and total anaerobes. High abundances of tet(Q), tet(M), tet(W), tet(O), and tet(B) were detected in the inoculum and also before antibiotic administration in the chemostat and did not significantly increase during administration of 1 and 4mg/L DOX. Only 16mg/L DOX caused minor enrichments. Conclusions: Cross-contamination concentrations of doxycycline, as a result of cross-contamination, cause a selection pressure for resistant bacteria and negatively affect microbial activity.
Keywords
TETRACYCLINE RESISTANCE GENES, ANTIBIOTIC-RESISTANCE, ANAEROBIC-DIGESTION, ANTIMICROBIAL USE, BACTERIA, FEED, PCR, CHLORTETRACYCLINE, EXTRACTION, INTESTINE, intestinal microbiology, tetracycline resistance, doxycycline, microbiome

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Citation

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MLA
De Mulder, Thijs et al. “Impact of Cross-contamination Concentrations of Doxycycline Hyclate on the Microbial Ecosystem in an Ex Vivo Model of the Pig’s Cecum.” MICROBIAL DRUG RESISTANCE 25.2 (2019): 304–315. Print.
APA
De Mulder, Thijs, Rasschaert, G., Van Coillie, E., Van den Meersche, T., Haegeman, A., Ruttink, T., Van de Wiele, T., et al. (2019). Impact of cross-contamination concentrations of doxycycline hyclate on the microbial ecosystem in an ex vivo model of the pig’s cecum. MICROBIAL DRUG RESISTANCE, 25(2), 304–315.
Chicago author-date
De Mulder, Thijs, Geertrui Rasschaert, Els Van Coillie, Tina Van den Meersche, Annelies Haegeman, Tom Ruttink, Tom Van de Wiele, and Marc Heyndrickx. 2019. “Impact of Cross-contamination Concentrations of Doxycycline Hyclate on the Microbial Ecosystem in an Ex Vivo Model of the Pig’s Cecum.” Microbial Drug Resistance 25 (2): 304–315.
Chicago author-date (all authors)
De Mulder, Thijs, Geertrui Rasschaert, Els Van Coillie, Tina Van den Meersche, Annelies Haegeman, Tom Ruttink, Tom Van de Wiele, and Marc Heyndrickx. 2019. “Impact of Cross-contamination Concentrations of Doxycycline Hyclate on the Microbial Ecosystem in an Ex Vivo Model of the Pig’s Cecum.” Microbial Drug Resistance 25 (2): 304–315.
Vancouver
1.
De Mulder T, Rasschaert G, Van Coillie E, Van den Meersche T, Haegeman A, Ruttink T, et al. Impact of cross-contamination concentrations of doxycycline hyclate on the microbial ecosystem in an ex vivo model of the pig’s cecum. MICROBIAL DRUG RESISTANCE. 2019;25(2):304–15.
IEEE
[1]
T. De Mulder et al., “Impact of cross-contamination concentrations of doxycycline hyclate on the microbial ecosystem in an ex vivo model of the pig’s cecum,” MICROBIAL DRUG RESISTANCE, vol. 25, no. 2, pp. 304–315, 2019.
@article{8610524,
  abstract     = {Aims: Cross-contamination of feed with antibiotics causes pigs to become unintentionally exposed to low concentrations of antibiotics. This study investigates the effect of residues of doxycycline hyclate (DOX) in an ex vivo model of the intestinal tract of pigs, focusing on the microbial community, microbial activity, and the enrichment of resistant bacteria and resistance genes.
Results: The effect of three concentrations DOX were tested; 1 and 4mg/L correspond to the intestinal concentrations when pigs are fed a compound feed containing 3% of a therapeutic dose, and a reference concentration of 16mg/L. These were continuously administered to a chemostat, simulating the microbial ecosystem of the pig cecum and inoculated with cecal content of organically grown pigs. The administration of even the lowest DOX concentration caused a significant decrease in bacterial activity, while the microbial community profile appeared to remain unaffected by any of the concentrations. A concentration of 1mg/L DOX caused minor selection pressure for tetracycline-resistant Escherichia coli but no other groups enumerated with plate cultivation, while 4mg/L induced major enrichment of tetracycline-resistant E. coli, Enterobacteriaceae and total anaerobes. High abundances of tet(Q), tet(M), tet(W), tet(O), and tet(B) were detected in the inoculum and also before antibiotic administration in the chemostat and did not significantly increase during administration of 1 and 4mg/L DOX. Only 16mg/L DOX caused minor enrichments.
Conclusions: Cross-contamination concentrations of doxycycline, as a result of cross-contamination, cause a selection pressure for resistant bacteria and negatively affect microbial activity.},
  author       = {De Mulder, Thijs and Rasschaert, Geertrui and Van Coillie, Els and Van den Meersche, Tina and Haegeman, Annelies and Ruttink, Tom and Van de Wiele, Tom and Heyndrickx, Marc},
  issn         = {1076-6294},
  journal      = {MICROBIAL DRUG RESISTANCE},
  keywords     = {TETRACYCLINE RESISTANCE GENES,ANTIBIOTIC-RESISTANCE,ANAEROBIC-DIGESTION,ANTIMICROBIAL USE,BACTERIA,FEED,PCR,CHLORTETRACYCLINE,EXTRACTION,INTESTINE,intestinal microbiology,tetracycline resistance,doxycycline,microbiome},
  language     = {eng},
  number       = {2},
  pages        = {304--315},
  title        = {Impact of cross-contamination concentrations of doxycycline hyclate on the microbial ecosystem in an ex vivo model of the pig's cecum},
  url          = {http://dx.doi.org/10.1089/mdr.2018.0034},
  volume       = {25},
  year         = {2019},
}

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