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Labelling halophilic Archaea using 13C and 15N stable isotopes : a potential tool to investigate haloarchaea consumption by metazoans

(2019) EXTREMOPHILES. 23(3). p.359-365
Author
Organization
Abstract
The use of stable isotope (SI) labelling and tracing of live diets is currently considered one of the most comprehensive tools to detect their uptake and assimilation by aquatic organisms. These techniques are indeed widely used in nutritional studies to follow the fate of specific microbial dietary components, unraveling trophic interactions. Nevertheless, to the current date our understanding of aquatic trophic relationships has yet to include a whole domain of life, the Archaea. The aim of the present research was, therefore, to describe a halophilic Archaea (haloarchaea) labelling procedure, using the SI C-13 and N-15, to enable the application of SI tracing in future studies of haloarchaea consumption by aquatic metazoans. To this end, three C-13 enriched carbon sources and two N-15 enriched nitrogen sources were tested as potential labels to enrich cells of three haloarchaea strains when supplemented to the culture medium. Our overall results indicate C-13-glycerol as the most effective carbon source to achieve an efficient C-13 enrichment in haloarchaea cells, with Delta delta C-13 values above 5000 parts per thousand in all tested haloarchaea strains. As for N-15 enriched nitrogen sources, both ((NH4)-N-15)(2)SO4 and (NH4Cl)-N-15 seem to be readily assimilated, also resulting in efficient N-15 enrichment in haloarchaea cells, with Delta delta N-15 values higher than 20,000 parts per thousand. We believe that the proposed methodology will allow for the use of SI labelled haloarchaea biomass in feeding tests, potentially providing unambiguous confirmation of the assimilation of haloarchaea biomass by aquatic metazoans.
Keywords
Haloarchaea, Stable isotopes, C-13, N-15, Isotope enrichment, Labelled Archaea, FOOD WEBS, METABOLISM, HALOBACTERIUM, ORGANISMS, DIVERSITY, BACTERIA, PATHWAYS, AMMONIUM, CARBON, NOV

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MLA
Alfama Lopes Dos Santos, Ruy Miguel et al. “Labelling Halophilic Archaea Using 13C and 15N Stable Isotopes : a Potential Tool to Investigate Haloarchaea Consumption by Metazoans.” EXTREMOPHILES 23.3 (2019): 359–365. Print.
APA
Alfama Lopes Dos Santos, R. M., De Troch, M., Bossier, P., & Van Stappen, G. (2019). Labelling halophilic Archaea using 13C and 15N stable isotopes : a potential tool to investigate haloarchaea consumption by metazoans. EXTREMOPHILES, 23(3), 359–365.
Chicago author-date
Alfama Lopes Dos Santos, Ruy Miguel, Marleen De Troch, Peter Bossier, and Gilbert Van Stappen. 2019. “Labelling Halophilic Archaea Using 13C and 15N Stable Isotopes : a Potential Tool to Investigate Haloarchaea Consumption by Metazoans.” Extremophiles 23 (3): 359–365.
Chicago author-date (all authors)
Alfama Lopes Dos Santos, Ruy Miguel, Marleen De Troch, Peter Bossier, and Gilbert Van Stappen. 2019. “Labelling Halophilic Archaea Using 13C and 15N Stable Isotopes : a Potential Tool to Investigate Haloarchaea Consumption by Metazoans.” Extremophiles 23 (3): 359–365.
Vancouver
1.
Alfama Lopes Dos Santos RM, De Troch M, Bossier P, Van Stappen G. Labelling halophilic Archaea using 13C and 15N stable isotopes : a potential tool to investigate haloarchaea consumption by metazoans. EXTREMOPHILES. 2019;23(3):359–65.
IEEE
[1]
R. M. Alfama Lopes Dos Santos, M. De Troch, P. Bossier, and G. Van Stappen, “Labelling halophilic Archaea using 13C and 15N stable isotopes : a potential tool to investigate haloarchaea consumption by metazoans,” EXTREMOPHILES, vol. 23, no. 3, pp. 359–365, 2019.
@article{8609946,
  abstract     = {The use of stable isotope (SI) labelling and tracing of live diets is currently considered one of the most comprehensive tools to detect their uptake and assimilation by aquatic organisms. These techniques are indeed widely used in nutritional studies to follow the fate of specific microbial dietary components, unraveling trophic interactions. Nevertheless, to the current date our understanding of aquatic trophic relationships has yet to include a whole domain of life, the Archaea. The aim of the present research was, therefore, to describe a halophilic Archaea (haloarchaea) labelling procedure, using the SI C-13 and N-15, to enable the application of SI tracing in future studies of haloarchaea consumption by aquatic metazoans. To this end, three C-13 enriched carbon sources and two N-15 enriched nitrogen sources were tested as potential labels to enrich cells of three haloarchaea strains when supplemented to the culture medium. Our overall results indicate C-13-glycerol as the most effective carbon source to achieve an efficient C-13 enrichment in haloarchaea cells, with Delta delta C-13 values above 5000 parts per thousand in all tested haloarchaea strains. As for N-15 enriched nitrogen sources, both ((NH4)-N-15)(2)SO4 and (NH4Cl)-N-15 seem to be readily assimilated, also resulting in efficient N-15 enrichment in haloarchaea cells, with Delta delta N-15 values higher than 20,000 parts per thousand. We believe that the proposed methodology will allow for the use of SI labelled haloarchaea biomass in feeding tests, potentially providing unambiguous confirmation of the assimilation of haloarchaea biomass by aquatic metazoans.},
  author       = {Alfama Lopes Dos Santos, Ruy Miguel and De Troch, Marleen and Bossier, Peter and Van Stappen, Gilbert},
  issn         = {1431-0651},
  journal      = {EXTREMOPHILES},
  keywords     = {Haloarchaea,Stable isotopes,C-13,N-15,Isotope enrichment,Labelled Archaea,FOOD WEBS,METABOLISM,HALOBACTERIUM,ORGANISMS,DIVERSITY,BACTERIA,PATHWAYS,AMMONIUM,CARBON,NOV},
  language     = {eng},
  number       = {3},
  pages        = {359--365},
  title        = {Labelling halophilic Archaea using 13C and 15N stable isotopes : a potential tool to investigate haloarchaea consumption by metazoans},
  url          = {http://dx.doi.org/10.1007/s00792-019-01084-w},
  volume       = {23},
  year         = {2019},
}

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