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Damage on plants activates Ca2+-dependent metacaspases for release of immunomodulatory peptides

Tim Hander, Alvaro Daniel Fernandez (UGent) , Robert Kumpf (UGent) , Patrick Willems (UGent) , Hendrik Schatowitz, Debbie Rombaut (UGent) , An Staes (UGent) , Jonah Nolf (UGent) , Robin Pottie (UGent) , Panfeng Yao (UGent) , et al.
(2019) SCIENCE. 363(6433).
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Abstract
Physical damage to cells leads to the release of immunomodulatory peptides to elicit a wound defense response in the surrounding tissue. In Arabidopsis thaliana, the plant elicitor peptide 1 (Pep1) is processed from its protein precursor, PRECURSOR OF PEP1 (PROPEP1). We demonstrate that upon damage, both at the tissue and single-cell levels, the cysteine protease METACASPASE4 (MC4) is instantly and spatiotemporally activated by binding high levels of Ca2+ and is necessary and sufficient for Pep1 maturation. Cytosol-localized PROPEP1 and MC4 react only after loss of plasma membrane integrity and prolonged extracellular Ca2+ entry. Our results reveal that a robust mechanism consisting of conserved molecular components links the intracellular and Ca2+-dependent activation of a specific cysteine protease with the maturation of damage-induced wound defense signals.
Keywords
Multidisciplinary

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MLA
Hander, Tim et al. “Damage on Plants Activates Ca2+-dependent Metacaspases for Release of Immunomodulatory Peptides.” SCIENCE 363.6433 (2019): n. pag. Print.
APA
Hander, T., Fernandez, A. D., Kumpf, R., Willems, P., Schatowitz, H., Rombaut, D., Staes, A., et al. (2019). Damage on plants activates Ca2+-dependent metacaspases for release of immunomodulatory peptides. SCIENCE, 363(6433).
Chicago author-date
Hander, Tim, Alvaro Daniel Fernandez, Robert Kumpf, Patrick Willems, Hendrik Schatowitz, Debbie Rombaut, An Staes, et al. 2019. “Damage on Plants Activates Ca2+-dependent Metacaspases for Release of Immunomodulatory Peptides.” Science 363 (6433).
Chicago author-date (all authors)
Hander, Tim, Alvaro Daniel Fernandez, Robert Kumpf, Patrick Willems, Hendrik Schatowitz, Debbie Rombaut, An Staes, Jonah Nolf, Robin Pottie, Panfeng Yao, Amanda Gonçalves, Benjamin Pavie, Thomas Boller, Kris Gevaert, Frank Van Breusegem, Sebastian Bartels, and Simon Stael. 2019. “Damage on Plants Activates Ca2+-dependent Metacaspases for Release of Immunomodulatory Peptides.” Science 363 (6433).
Vancouver
1.
Hander T, Fernandez AD, Kumpf R, Willems P, Schatowitz H, Rombaut D, et al. Damage on plants activates Ca2+-dependent metacaspases for release of immunomodulatory peptides. SCIENCE. 2019;363(6433).
IEEE
[1]
T. Hander et al., “Damage on plants activates Ca2+-dependent metacaspases for release of immunomodulatory peptides,” SCIENCE, vol. 363, no. 6433, 2019.
@article{8609893,
  abstract     = {Physical damage to cells leads to the release of immunomodulatory peptides to elicit a wound defense response in the surrounding tissue. In Arabidopsis thaliana, the plant elicitor peptide 1 (Pep1) is processed from its protein precursor, PRECURSOR OF PEP1 (PROPEP1). We demonstrate that upon damage, both at the tissue and single-cell levels, the cysteine protease METACASPASE4 (MC4) is instantly and spatiotemporally activated by binding high levels of Ca2+ and is necessary and sufficient for Pep1 maturation. Cytosol-localized PROPEP1 and MC4 react only after loss of plasma membrane integrity and prolonged extracellular Ca2+ entry. Our results reveal that a robust mechanism consisting of conserved molecular components links the intracellular and Ca2+-dependent activation of a specific cysteine protease with the maturation of damage-induced wound defense signals.},
  articleno    = {eaar7486},
  author       = {Hander, Tim and Fernandez, Alvaro Daniel and Kumpf, Robert and Willems, Patrick and Schatowitz, Hendrik and Rombaut, Debbie and Staes, An and Nolf, Jonah and Pottie, Robin and Yao, Panfeng and Gonçalves, Amanda and Pavie, Benjamin and Boller, Thomas and Gevaert, Kris and Van Breusegem, Frank and Bartels, Sebastian and Stael, Simon},
  issn         = {0036-8075},
  journal      = {SCIENCE},
  keywords     = {Multidisciplinary},
  language     = {eng},
  number       = {6433},
  pages        = {10},
  title        = {Damage on plants activates Ca2+-dependent metacaspases for release of immunomodulatory peptides},
  url          = {http://dx.doi.org/10.1126/science.aar7486},
  volume       = {363},
  year         = {2019},
}

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