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Survival of single positive thymocytes depends upon developmental control of RIPK1 kinase signaling by the IKK complex independent of NF-κB

(2019) IMMUNITY. 50(2). p.348-361
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Abstract
NF-kappa B(nuclear factor kappa B) signaling is considered critical for single positive (SP) thymocyte development because loss of upstream activators of NF-kappa B, such as the IKK complex, arrests their development. We found that the compound ablation of RelA, cRel, and p50, required for canonical NF-kappa B transcription, had no impact upon thymocyte development. While IKK-deficient thymocytes were acutely sensitive to tumor necrosis factor (TNF)-induced cell death, Rel-deficient cells remained resistant, calling into question the importance of NF-kappa B as the IKK target required for thymocyte survival. Instead, we found that IKK controlled thymocyte survival by repressing cell-death-inducing activity of the serine/threonine kinase RIPK1. We observed that RIPK1 expression was induced during development of SP thymocytes and that IKK was required to prevent RIPK1-kinase-dependent death of SPs in vivo. Finally, we showed that IKK was required to protect Rel-deficient thymocytes from RIPK1-dependent cell death, underscoring the NF-kappa B-independent function of IKK during thymic development.
Keywords
T-CELLS, DEATH, NECROPTOSIS, ACTIVATION, APOPTOSIS, SELECTION, INNATE, TAK1, DIFFERENTIATION, MECHANISMS

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MLA
Webb, Louise V et al. “Survival of Single Positive Thymocytes Depends Upon Developmental Control of RIPK1 Kinase Signaling by the IKK Complex Independent of NF-κB.” IMMUNITY 50.2 (2019): 348–361. Print.
APA
Webb, L. V., Barbarulo, A., Huysentruyt, J., Vanden Berghe, T., Takahashi, N., Ley, S., Vandenabeele, P., et al. (2019). Survival of single positive thymocytes depends upon developmental control of RIPK1 kinase signaling by the IKK complex independent of NF-κB. IMMUNITY, 50(2), 348–361.
Chicago author-date
Webb, Louise V, Alessandro Barbarulo, Jelle Huysentruyt, Tom Vanden Berghe, Nozomi Takahashi, Steven Ley, Peter Vandenabeele, and Benedict Seddon. 2019. “Survival of Single Positive Thymocytes Depends Upon Developmental Control of RIPK1 Kinase Signaling by the IKK Complex Independent of NF-κB.” Immunity 50 (2): 348–361.
Chicago author-date (all authors)
Webb, Louise V, Alessandro Barbarulo, Jelle Huysentruyt, Tom Vanden Berghe, Nozomi Takahashi, Steven Ley, Peter Vandenabeele, and Benedict Seddon. 2019. “Survival of Single Positive Thymocytes Depends Upon Developmental Control of RIPK1 Kinase Signaling by the IKK Complex Independent of NF-κB.” Immunity 50 (2): 348–361.
Vancouver
1.
Webb LV, Barbarulo A, Huysentruyt J, Vanden Berghe T, Takahashi N, Ley S, et al. Survival of single positive thymocytes depends upon developmental control of RIPK1 kinase signaling by the IKK complex independent of NF-κB. IMMUNITY. 2019;50(2):348–61.
IEEE
[1]
L. V. Webb et al., “Survival of single positive thymocytes depends upon developmental control of RIPK1 kinase signaling by the IKK complex independent of NF-κB,” IMMUNITY, vol. 50, no. 2, pp. 348–361, 2019.
@article{8608925,
  abstract     = {NF-kappa B(nuclear factor kappa B) signaling is considered critical for single positive (SP) thymocyte development because loss of upstream activators of NF-kappa B, such as the IKK complex, arrests their development. We found that the compound ablation of RelA, cRel, and p50, required for canonical NF-kappa B transcription, had no impact upon thymocyte development. While IKK-deficient thymocytes were acutely sensitive to tumor necrosis factor (TNF)-induced cell death, Rel-deficient cells remained resistant, calling into question the importance of NF-kappa B as the IKK target required for thymocyte survival. Instead, we found that IKK controlled thymocyte survival by repressing cell-death-inducing activity of the serine/threonine kinase RIPK1. We observed that RIPK1 expression was induced during development of SP thymocytes and that IKK was required to prevent RIPK1-kinase-dependent death of SPs in vivo. Finally, we showed that IKK was required to protect Rel-deficient thymocytes from RIPK1-dependent cell death, underscoring the NF-kappa B-independent function of IKK during thymic development.},
  author       = {Webb, Louise V and Barbarulo, Alessandro and Huysentruyt, Jelle and Vanden Berghe, Tom and Takahashi, Nozomi and Ley, Steven and Vandenabeele, Peter and Seddon, Benedict},
  issn         = {1074-7613},
  journal      = {IMMUNITY},
  keywords     = {T-CELLS,DEATH,NECROPTOSIS,ACTIVATION,APOPTOSIS,SELECTION,INNATE,TAK1,DIFFERENTIATION,MECHANISMS},
  language     = {eng},
  number       = {2},
  pages        = {348--361},
  title        = {Survival of single positive thymocytes depends upon developmental control of RIPK1 kinase signaling by the IKK complex independent of NF-κB},
  url          = {http://dx.doi.org/10.1016/j.immuni.2019.01.004},
  volume       = {50},
  year         = {2019},
}

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