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Biomimetic strategy towards gelatin coatings on PET : effect of protocol on coating stability and cell-interactive properties

(2019) JOURNAL OF MATERIALS CHEMISTRY B. 7(8). p.1258-1269
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Abstract
Gelatin-modified poly(ethylene terephthalate) (PET) surfaces have been previously realized via an intermediate dopamine coating procedure that resulted in surfaces with superior haemocompatibility compared to unfunctionalized PET. The present study addresses the biocompatibility assessment of these coated PET surfaces. In this context, the stability of the gelatin coating upon exposure to physiological conditions and its cell-interactive properties were investigated. The proposed gelatindopamine-PET surfaces showed an increased protein coating stability up to 24 days and promoted the attachment and spreading of both endothelial cells (ECs) and smooth muscle cells (SMCs). In parallel, physisorbed gelatin coatings exhibited similar cell-interactive properties, albeit temporarily, as the coating delaminated within 1 week after cell seeding. Furthermore, no or only minimal immunogenic or inflammatory responses were observed during in vitro cytotoxicity and endotoxicity assessment for all gelatin-modified PET surfaces evaluated. Overall, the combined enhanced biocompatibility reported herein together with the previously proven haemocompatibility show the potential of the gelatin-dopaminePET surfaces to function as vascular graft candidates.
Keywords
SURFACE MODIFICATION, CHEMISTRY, POLYESTER, ADHESION, FUNCTIONALIZATION, BIOCOMPATIBILITY, IMMOBILIZATION, PROLIFERATION, COMPATIBILITY, BIOMATERIALS

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MLA
Giol, Elena Diana et al. “Biomimetic Strategy Towards Gelatin Coatings on PET : Effect of Protocol on Coating Stability and Cell-interactive Properties.” JOURNAL OF MATERIALS CHEMISTRY B 7.8 (2019): 1258–1269. Print.
APA
Giol, E. D., Van Vlierberghe, S., Unger, R. E., Kersemans, K., De Vos, F., Kirkpatrick, C. J., & Dubruel, P. (2019). Biomimetic strategy towards gelatin coatings on PET : effect of protocol on coating stability and cell-interactive properties. JOURNAL OF MATERIALS CHEMISTRY B, 7(8), 1258–1269.
Chicago author-date
Giol, Elena Diana, Sandra Van Vlierberghe, Ronald E Unger, Ken Kersemans, Filip De Vos, Charles James Kirkpatrick, and Peter Dubruel. 2019. “Biomimetic Strategy Towards Gelatin Coatings on PET : Effect of Protocol on Coating Stability and Cell-interactive Properties.” Journal of Materials Chemistry B 7 (8): 1258–1269.
Chicago author-date (all authors)
Giol, Elena Diana, Sandra Van Vlierberghe, Ronald E Unger, Ken Kersemans, Filip De Vos, Charles James Kirkpatrick, and Peter Dubruel. 2019. “Biomimetic Strategy Towards Gelatin Coatings on PET : Effect of Protocol on Coating Stability and Cell-interactive Properties.” Journal of Materials Chemistry B 7 (8): 1258–1269.
Vancouver
1.
Giol ED, Van Vlierberghe S, Unger RE, Kersemans K, De Vos F, Kirkpatrick CJ, et al. Biomimetic strategy towards gelatin coatings on PET : effect of protocol on coating stability and cell-interactive properties. JOURNAL OF MATERIALS CHEMISTRY B. 2019;7(8):1258–69.
IEEE
[1]
E. D. Giol et al., “Biomimetic strategy towards gelatin coatings on PET : effect of protocol on coating stability and cell-interactive properties,” JOURNAL OF MATERIALS CHEMISTRY B, vol. 7, no. 8, pp. 1258–1269, 2019.
@article{8608264,
  abstract     = {Gelatin-modified poly(ethylene terephthalate) (PET) surfaces have been previously realized via an intermediate dopamine coating procedure that resulted in surfaces with superior haemocompatibility compared to unfunctionalized PET. The present study addresses the biocompatibility assessment of these coated PET surfaces. In this context, the stability of the gelatin coating upon exposure to physiological conditions and its cell-interactive properties were investigated. The proposed gelatindopamine-PET surfaces showed an increased protein coating stability up to 24 days and promoted the attachment and spreading of both endothelial cells (ECs) and smooth muscle cells (SMCs). In parallel, physisorbed gelatin coatings exhibited similar cell-interactive properties, albeit temporarily, as the coating delaminated within 1 week after cell seeding. Furthermore, no or only minimal immunogenic or inflammatory responses were observed during in vitro cytotoxicity and endotoxicity assessment for all gelatin-modified PET surfaces evaluated. Overall, the combined enhanced biocompatibility reported herein together with the previously proven haemocompatibility show the potential of the gelatin-dopaminePET surfaces to function as vascular graft candidates.},
  author       = {Giol, Elena Diana and Van Vlierberghe, Sandra and Unger, Ronald E and Kersemans, Ken and De Vos, Filip and Kirkpatrick, Charles James and Dubruel, Peter},
  issn         = {2050-750X},
  journal      = {JOURNAL OF MATERIALS CHEMISTRY B},
  keywords     = {SURFACE MODIFICATION,CHEMISTRY,POLYESTER,ADHESION,FUNCTIONALIZATION,BIOCOMPATIBILITY,IMMOBILIZATION,PROLIFERATION,COMPATIBILITY,BIOMATERIALS},
  language     = {eng},
  number       = {8},
  pages        = {1258--1269},
  title        = {Biomimetic strategy towards gelatin coatings on PET : effect of protocol on coating stability and cell-interactive properties},
  url          = {http://dx.doi.org/10.1039/c8tb02676a},
  volume       = {7},
  year         = {2019},
}

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