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Breed differences in PCV2 uptake and disintegration in porcine monocytes

Ruifang Wei (UGent) , Ivan Trus (UGent) , Bo Yang (UGent) , Liping Huang and Hans Nauwynck (UGent)
(2018) VIRUSES-BASEL. 10(10).
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Abstract
Porcine circovirus type 2 (PCV2) is associated with various diseases which are designated as PCV2-associated diseases (PCVADs). Their severity varies among breeds. In the diseased pigs, virus is present in monocytes, without replication or full degradation. PCV2 entry and viral outcome in primary porcine monocytes and the role of monocytes in PCV2 genetic susceptibility have not been studied. Here, virus uptake and trafficking were analyzed and compared among purebreds Pietrain, Landrace and Large White and hybrid Pietrain x Topigs20. Viral capsids were rapidly internalized into monocytes, followed by a slow disintegration to a residual level. PCV2 uptake was decreased by chlorpromazine, cytochalasin D and dynasore. The internalized capsids followed the endosomal trafficking pathway, ending up in lysosomes. PCV2 genome was nicked by lysosomal DNase II in vitro, but persisted in monocytes in vivo. Monocytes from purebred Pietrain and the hybrid showed a higher level of PCV2 uptake and disintegration, compared to those from Landrace and Large White. In conclusion, PCV2 entry occurs via clathrin-mediated endocytosis. After entry, viral capsids are partially disintegrated, while viral genomes largely escape from the pathway to avoid degradation. The degree of PCV2 uptake and disintegration differ among pig breeds.
Keywords
CIRCOVIRUS TYPE-2 REPLICATION, CLATHRIN-MEDIATED ENDOCYTOSIS, WASTING, SYNDROME PMWS, RESPIRATORY SYNDROME VIRUS, IN-VITRO, ALVEOLAR, MACROPHAGES, CELL-LINES, MONOCLONAL-ANTIBODIES, PATHOLOGICAL LESIONS, PERSISTENT INFECTION, porcine circovirus type 2, blood monocytes, uptake, disintegration, viral capsids, viral genomes, breeds

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Citation

Please use this url to cite or link to this publication:

Chicago
Wei, Ruifang, Ivan Trus, Bo Yang, Liping Huang, and Hans Nauwynck. 2018. “Breed Differences in PCV2 Uptake and Disintegration in Porcine Monocytes.” Viruses-basel 10 (10).
APA
Wei, R., Trus, I., Yang, B., Huang, L., & Nauwynck, H. (2018). Breed differences in PCV2 uptake and disintegration in porcine monocytes. VIRUSES-BASEL, 10(10).
Vancouver
1.
Wei R, Trus I, Yang B, Huang L, Nauwynck H. Breed differences in PCV2 uptake and disintegration in porcine monocytes. VIRUSES-BASEL. 2018;10(10).
MLA
Wei, Ruifang et al. “Breed Differences in PCV2 Uptake and Disintegration in Porcine Monocytes.” VIRUSES-BASEL 10.10 (2018): n. pag. Print.
@article{8607910,
  abstract     = {Porcine circovirus type 2 (PCV2) is associated with various diseases which are designated as PCV2-associated diseases (PCVADs). Their severity varies among breeds. In the diseased pigs, virus is present in monocytes, without replication or full degradation. PCV2 entry and viral outcome in primary porcine monocytes and the role of monocytes in PCV2 genetic susceptibility have not been studied. Here, virus uptake and trafficking were analyzed and compared among purebreds Pietrain, Landrace and Large White and hybrid Pietrain x Topigs20. Viral capsids were rapidly internalized into monocytes, followed by a slow disintegration to a residual level. PCV2 uptake was decreased by chlorpromazine, cytochalasin D and dynasore. The internalized capsids followed the endosomal trafficking pathway, ending up in lysosomes. PCV2 genome was nicked by lysosomal DNase II in vitro, but persisted in monocytes in vivo. Monocytes from purebred Pietrain and the hybrid showed a higher level of PCV2 uptake and disintegration, compared to those from Landrace and Large White. In conclusion, PCV2 entry occurs via clathrin-mediated endocytosis. After entry, viral capsids are partially disintegrated, while viral genomes largely escape from the pathway to avoid degradation. The degree of PCV2 uptake and disintegration differ among pig breeds.},
  articleno    = {562},
  author       = {Wei, Ruifang and Trus, Ivan and Yang, Bo and Huang, Liping and Nauwynck, Hans},
  issn         = {1999-4915},
  journal      = {VIRUSES-BASEL},
  language     = {eng},
  number       = {10},
  pages        = {21},
  title        = {Breed differences in PCV2 uptake and disintegration in porcine monocytes},
  url          = {http://dx.doi.org/10.3390/v10100562},
  volume       = {10},
  year         = {2018},
}

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