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Supplementation of DHA-Gold pre and/or postnatally to goat kids modifies in vitro methane production and rumen morphology until 6 mo old

(2018) JOURNAL OF ANIMAL SCIENCE. 96(11). p.4845-4858
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Abstract
This study aimed to investigate the effect of pre and/or postnatal supplementation of a dry whole cell algae (DHA-Cold) to goat kids, on in vitro methane (CH4) production, animal growth, and rumen morphology at the age of 6 mo. Furthermore, the in vitro retreatment effect of DHA-Gold was evaluated. Twenty pregnant Saanen goats giving birth to 2 male kids were used. Half of these does were supplemented (D+) with 18.2 g/d of DHA-Gold in the last 3 wk of pregnancy, whereas the other half was not (D). After kidding, one goat kid per doe in both groups was supplemented daily with 0.28 g/kg of body weight of DHA-Gold (k+) until 12 wk, whereas the other goat kids were untreated (k). This resulted in 4 experimental groups D+k+, D+k, D k+, and D k, In vitro incubations were performed at the ages of 4 wk, 11 wk, and 6 mo. At the age of 6 mo, goat kids were euthanized and additional incubations were performed supplementing 4 doses of DHA-Gold (0, 0.4, 0.8, and 1.6 mg/mL). Additionally, rumen tissue of the atrium ruminis, ventral rumen, and dorsal blind sac were collected to assess rumen morphology. Rumen inocula of 4-wk-old goat kids supplemented D+ showed lower (P < 0.05) in vitro CH4 production, however, this was mainly due to a reduction in the overall fermentation, while CH4 expressed relatively to total volatile fatty acids (VFA) was higher when goat kids were treated D+ or k+. The detrimental D+ effect on VFA production diminished at 11 wk old but remained a tendency (0.05 < P < 0.1). As for 4 wk D+ as well as k+ supplementation of DHA-Gold stimulated rather than inhibited in vitro CH4 production expressed relative to total VFA. Supplementation of DHA-Gold either D+ or k+ decreased density, width, and surface area of the ruminal papillae. However, no effect on animal growth was observed. Moreover, detrimental effects of D+ or k+ treatment on VFA production or stimulation of relative CH4 production were no longer observed at 6 mo old. Nevertheless, direct exposure of DHA-Gold to 6-mo-old inoculum linearly (P < 0.05) decreased CH4 and VFA production, which tended (P = 0.06) to be greater when using D-rumen inoculum. Accordingly, neither D+ nor k+ DHA-Gold supplementation showed potential for reduction of rumen methanogenesis. Furthermore, this early life intervention could represent some risk for impaired rumen papillae development, which, however, did not impair animal performance.
Keywords
POLYUNSATURATED FATTY-ACIDS, DOCOSAHEXAENOIC ACID, DRY PERIOD, EARLY-LIFE, PERFORMANCE, DIET, FERMENTATION, ADAPTATION, ABSORPTION, MICROALGAE, DHA-Gold, goats, in vitro methane, pre/postnatal supplementation, retreatment, rumen papillae

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Citation

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Chicago
Ruiz González, Alexis, Sieglinde Debruyne, Lore Dewanckele, M Escobar, L. Vandaele, Wim Van Den Broeck, and Veerle Fievez. 2018. “Supplementation of DHA-Gold Pre And/or Postnatally to Goat Kids Modifies in Vitro Methane Production and Rumen Morphology Until 6 Mo Old.” Journal of Animal Science 96 (11): 4845–4858.
APA
Ruiz González, A., Debruyne, S., Dewanckele, L., Escobar, M., Vandaele, L., Van Den Broeck, W., & Fievez, V. (2018). Supplementation of DHA-Gold pre and/or postnatally to goat kids modifies in vitro methane production and rumen morphology until 6 mo old. JOURNAL OF ANIMAL SCIENCE, 96(11), 4845–4858.
Vancouver
1.
Ruiz González A, Debruyne S, Dewanckele L, Escobar M, Vandaele L, Van Den Broeck W, et al. Supplementation of DHA-Gold pre and/or postnatally to goat kids modifies in vitro methane production and rumen morphology until 6 mo old. JOURNAL OF ANIMAL SCIENCE. 2018;96(11):4845–58.
MLA
Ruiz González, Alexis et al. “Supplementation of DHA-Gold Pre And/or Postnatally to Goat Kids Modifies in Vitro Methane Production and Rumen Morphology Until 6 Mo Old.” JOURNAL OF ANIMAL SCIENCE 96.11 (2018): 4845–4858. Print.
@article{8607051,
  abstract     = {This study aimed to investigate the effect of pre and/or postnatal supplementation of a dry whole cell algae (DHA-Cold) to goat kids, on in vitro methane (CH4) production, animal growth, and rumen morphology at the age of 6 mo. Furthermore, the in vitro retreatment effect of DHA-Gold was evaluated. Twenty pregnant Saanen goats giving birth to 2 male kids were used. Half of these does were supplemented (D+) with 18.2 g/d of DHA-Gold in the last 3 wk of pregnancy, whereas the other half was not (D). After kidding, one goat kid per doe in both groups was supplemented daily with 0.28 g/kg of body weight of DHA-Gold (k+) until 12 wk, whereas the other goat kids were untreated (k). This resulted in 4 experimental groups D+k+, D+k, D k+, and D k, In vitro incubations were performed at the ages of 4 wk, 11 wk, and 6 mo. At the age of 6 mo, goat kids were euthanized and additional incubations were performed supplementing 4 doses of DHA-Gold (0, 0.4, 0.8, and 1.6 mg/mL). Additionally, rumen tissue of the atrium ruminis, ventral rumen, and dorsal blind sac were collected to assess rumen morphology. Rumen inocula of 4-wk-old goat kids supplemented D+ showed lower (P < 0.05) in vitro CH4 production, however, this was mainly due to a reduction in the overall fermentation, while CH4 expressed relatively to total volatile fatty acids (VFA) was higher when goat kids were treated D+ or k+. The detrimental D+ effect on VFA production diminished at 11 wk old but remained a tendency (0.05 < P < 0.1). As for 4 wk D+ as well as k+ supplementation of DHA-Gold stimulated rather than inhibited in vitro CH4 production expressed relative to total VFA. Supplementation of DHA-Gold either D+ or k+ decreased density, width, and surface area of the ruminal papillae. However, no effect on animal growth was observed. Moreover, detrimental effects of D+ or k+ treatment on VFA production or stimulation of relative CH4 production were no longer observed at 6 mo old. Nevertheless, direct exposure of DHA-Gold to 6-mo-old inoculum linearly (P < 0.05) decreased CH4 and VFA production, which tended (P = 0.06) to be greater when using D-rumen inoculum. Accordingly, neither D+ nor k+ DHA-Gold supplementation showed potential for reduction of rumen methanogenesis. Furthermore, this early life intervention could represent some risk for impaired rumen papillae development, which, however, did not impair animal performance.},
  author       = {Ruiz González, Alexis and Debruyne, Sieglinde and Dewanckele, Lore and Escobar, M and Vandaele, L. and Van Den Broeck, Wim and Fievez, Veerle},
  issn         = {0021-8812},
  journal      = {JOURNAL OF ANIMAL SCIENCE},
  keywords     = {POLYUNSATURATED FATTY-ACIDS,DOCOSAHEXAENOIC ACID,DRY PERIOD,EARLY-LIFE,PERFORMANCE,DIET,FERMENTATION,ADAPTATION,ABSORPTION,MICROALGAE,DHA-Gold,goats,in vitro methane,pre/postnatal supplementation,retreatment,rumen papillae},
  language     = {eng},
  number       = {11},
  pages        = {4845--4858},
  title        = {Supplementation of DHA-Gold pre and/or postnatally to goat kids modifies in vitro methane production and rumen morphology until 6 mo old},
  url          = {http://dx.doi.org/10.1093/jas/sky307},
  volume       = {96},
  year         = {2018},
}

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