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Proteolytic processing of SERK3/BAK1 regulates plant immunity, development and cell death

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Abstract
Plants have evolved many receptor-like kinases (RLKs) to sense extrinsic and intrinsic cues. The signaling pathways mediated by multiple leucine-rich repeat (LRR) RLK (LRR-RLK) receptors require ligand-induced receptor-coreceptor heterodimerization and transphosphorylation with BAK1/SERK family LRR-RLKs. Here we reveal an additional layer of regulation of BAK1 via a Ca2+-dependent proteolytic cleavage process that is conserved in Arabidopsis thaliana, Nicotiana benthamiana and Saccharomyces cerevisiae . The proteolytic cleavage of BAK1 is intrinsically regulated in response to developmental cues and immune stimulation. The surface-exposed aspartic acid (D287) residue of BAK1 is critical for its proteolytic cleavage and plays an essential role in BAK1-regulated plant immunity, growth hormone brassinosteroid-mediated responses and cell death containment. BAK1D287A mutation impairs BAK1 phosphorylation on its substrate BIK1, and its plasma membrane (PM) localization. Intriguingly, it aggravates BAK1 overexpression-triggered cell death independent of BIK1, suggesting that maintaining homeostasis of BAK1 through a proteolytic process is crucial to control plant growth and immunity. Our data reveal that in addition to layered transphosphorylation in the receptor complexes, the proteolytic cleavage is an important regulatory process for the proper functions of the shared co-receptor BAK1 in diverse cellular signaling pathways.
Keywords
Plant Science, Genetics, Physiology

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Citation

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Chicago
Zhou, Jinggeng, Ping Wang, Lucas Alves Neubus Claus, Daniel Savatin, Guangyuan Xu, Shujing Wu, Xiangzong Meng, Eugenia Russinova, Ping He, and Libo Shan. 2019. “Proteolytic Processing of SERK3/BAK1 Regulates Plant Immunity, Development and Cell Death.” Plant Physiology.
APA
Zhou, J., Wang, P., Alves Neubus Claus, L., Savatin, D., Xu, G., Wu, S., Meng, X., et al. (2019). Proteolytic processing of SERK3/BAK1 regulates plant immunity, development and cell death. Plant Physiology.
Vancouver
1.
Zhou J, Wang P, Alves Neubus Claus L, Savatin D, Xu G, Wu S, et al. Proteolytic processing of SERK3/BAK1 regulates plant immunity, development and cell death. Plant Physiology. American Society of Plant Biologists (ASPB); 2019;
MLA
Zhou, Jinggeng et al. “Proteolytic Processing of SERK3/BAK1 Regulates Plant Immunity, Development and Cell Death.” Plant Physiology (2019): n. pag. Print.
@article{8606432,
  abstract     = {Plants have evolved many receptor-like kinases (RLKs) to sense extrinsic and intrinsic cues. The signaling pathways mediated by multiple leucine-rich repeat (LRR) RLK (LRR-RLK) receptors require ligand-induced receptor-coreceptor heterodimerization and transphosphorylation with BAK1/SERK family LRR-RLKs. Here we reveal an additional layer of regulation of BAK1 via a Ca2+-dependent proteolytic cleavage process that is conserved in Arabidopsis thaliana, Nicotiana benthamiana and Saccharomyces cerevisiae . The proteolytic cleavage of BAK1 is intrinsically regulated in response to developmental cues and immune stimulation. The surface-exposed aspartic acid (D287) residue of BAK1 is critical for its proteolytic cleavage and plays an essential role in BAK1-regulated plant immunity, growth hormone brassinosteroid-mediated responses and cell death containment. BAK1D287A mutation impairs BAK1 phosphorylation on its substrate BIK1, and its plasma membrane (PM) localization. Intriguingly, it aggravates BAK1 overexpression-triggered cell death independent of BIK1, suggesting that maintaining homeostasis of BAK1 through a proteolytic process is crucial to control plant growth and immunity. Our data reveal that in addition to layered transphosphorylation in the receptor complexes, the proteolytic cleavage is an important regulatory process for the proper functions of the shared co-receptor BAK1 in diverse cellular signaling pathways.},
  articleno    = {pp.01503.2018},
  author       = {Zhou, Jinggeng and Wang, Ping and Alves Neubus Claus, Lucas and Savatin, Daniel and Xu, Guangyuan and Wu, Shujing and Meng, Xiangzong and Russinova, Eugenia and He, Ping and Shan, Libo},
  issn         = {0032-0889},
  journal      = {Plant Physiology},
  publisher    = {American Society of Plant Biologists (ASPB)},
  title        = {Proteolytic processing of SERK3/BAK1 regulates plant immunity, development and cell death},
  url          = {http://dx.doi.org/10.1104/pp.18.01503},
  year         = {2019},
}

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