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Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

(2018) DEVELOPMENTAL BIOLOGY. 435(2). p.176-184
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Abstract
The capacity to fully replace teeth continuously makes zebrafish an attractive model to explore regeneration and tooth development. The requirement of attachment bone for the appearance of replacement teeth has been hypothesized but not yet investigated. The transcription factor sp7 (osterix) is known in mammals to play an important role during odontoblast differentiation and root formation. Here we study tooth replacement in the absence of attachment bone using sp7 zebrafish mutants. We analysed the pattern of tooth replacement at different stages of development and demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted. Without bone of attachment we observed abnormal orientation of teeth, and abnormal connection of pulp cavities of predecessor and replacement teeth. Mutants lacking sp7 show arrested dentinogenesis, with non-polarization of odontoblasts and only a thin layer of dentin deposited. Osteodast activity was observed in sp7 mutants; due to the lack of bone of attachment, remodelling was diminished but nevertheless present along the pharyngeal bone. We conclude that tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment. Without bone of attachment tooth orientation and pulp organization are compromised.
Keywords
DANIO-RERIO, PHARYNGEAL DENTITION, BONE-FORMATION, TELEOST FISH, OSTERIX, SKELETAL, REPLACEMENT, CELLS, CYPRINIDAE, EXPRESSION

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Chicago
Kague, E., Paul Witten, Mieke Soenens, C. L. Campos, T. Lubiana, S. Fisher, C. Hammond, K. Robson Brown, M. R. Passos-Bueno, and Ann Huysseune. 2018. “Zebrafish Sp7 Mutants Show Tooth Cycling Independent of Attachment, Eruption and Poor Differentiation of Teeth.” Developmental Biology 435 (2): 176–184.
APA
Kague, E., Witten, P., Soenens, M., Campos, C. L., Lubiana, T., Fisher, S., Hammond, C., et al. (2018). Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth. DEVELOPMENTAL BIOLOGY, 435(2), 176–184.
Vancouver
1.
Kague E, Witten P, Soenens M, Campos CL, Lubiana T, Fisher S, et al. Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth. DEVELOPMENTAL BIOLOGY. San diego: Academic Press Inc Elsevier Science; 2018;435(2):176–84.
MLA
Kague, E. et al. “Zebrafish Sp7 Mutants Show Tooth Cycling Independent of Attachment, Eruption and Poor Differentiation of Teeth.” DEVELOPMENTAL BIOLOGY 435.2 (2018): 176–184. Print.
@article{8606206,
  abstract     = {The capacity to fully replace teeth continuously makes zebrafish an attractive model to explore regeneration and tooth development. The requirement of attachment bone for the appearance of replacement teeth has been hypothesized but not yet investigated. The transcription factor sp7 (osterix) is known in mammals to play an important role during odontoblast differentiation and root formation. Here we study tooth replacement in the absence of attachment bone using sp7 zebrafish mutants. We analysed the pattern of tooth replacement at different stages of development and demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted. Without bone of attachment we observed abnormal orientation of teeth, and abnormal connection of pulp cavities of predecessor and replacement teeth. Mutants lacking sp7 show arrested dentinogenesis, with non-polarization of odontoblasts and only a thin layer of dentin deposited. Osteodast activity was observed in sp7 mutants; due to the lack of bone of attachment, remodelling was diminished but nevertheless present along the pharyngeal bone. We conclude that tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment. Without bone of attachment tooth orientation and pulp organization are compromised.},
  author       = {Kague, E. and Witten, Paul and Soenens, Mieke and Campos, C. L. and Lubiana, T. and Fisher, S. and Hammond, C. and Brown, K. Robson and Passos-Bueno, M. R. and Huysseune, Ann},
  issn         = {0012-1606},
  journal      = {DEVELOPMENTAL BIOLOGY},
  language     = {eng},
  number       = {2},
  pages        = {176--184},
  publisher    = {Academic Press Inc Elsevier Science},
  title        = {Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth},
  url          = {http://dx.doi.org/10.1016/j.ydbio.2018.01.021},
  volume       = {435},
  year         = {2018},
}

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