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A natural killer T-cell subset that protects against airway hyperreactivity

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Abstract
Background: Infection of suckling mice with influenza virus expands a CD4(-)CD8(-) double-negative (DN) natural killer T (NKT) cell subpopulation that protects the mice as adults against allergen-induced airway hyperreactivity (AHR). However, this NKT cell subset has not been characterized, and the underlying mechanisms of protection remain unknown. Objective: We characterized this specific NKT cell subpopulation that developed during influenza infection in neonatal mice and that suppressed the subsequent development of AHR. Methods: A cell-surface marker was identified by comparing the mRNA expression profile of wild-type CD4+ NKT cells with that of suppressive V alpha 14 DN NKT cells. The marker-enriched NKT cell subset was then analyzed for its cytokine profile and its suppressive in vitro and in vivo abilities. Results: We showed that DN NKT cells with high CD38 expression produced IFN-gamma, but not IL-17, IL-4, or IL-13, and inhibited development of AHR through contact-dependent suppression of helper CD4 T-cell proliferation. The NKT subset expanded in the lungs of neonatal mice after infection with influenza and also after treatment of neonatal mice with Nu-alpha-GalCer, which effectively increased DN CD38(hi) NKT cell numbers. Conclusion: These results suggest that early/neonatal exposure to infection or antigen challenge affects subsequent lung immunity by altering the cellular composition of cells in the lung and that some subsets of NKT cells suppress AHR. These results provide a possible mechanism by which prior infections can protect against the development of allergic asthma and might be further explored as a protective measure for young children.
Keywords
NKT CELLS, ALPHA-GALACTOSYLCERAMIDE, BONE-MARROW, EARLY-LIFE, ASTHMA, INNATE, EXPOSURE, LIGAND, ACTIVATION, MICE, Natural killer T subset, asthma, airway hyperreactivity, hygiene, hypothesis, influenza, CD38

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Citation

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MLA
Chuang, Ya-Ting et al. “A Natural Killer T-cell Subset That Protects Against Airway Hyperreactivity.” JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 143.2 (2019): 565–576. Print.
APA
Chuang, Y.-T., Leung, K., Chang, Y.-J., DeKruyff, R. H., Savage, P. B., Cruse, R., Benoit, C., et al. (2019). A natural killer T-cell subset that protects against airway hyperreactivity. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 143(2), 565–576.
Chicago author-date
Chuang, Ya-Ting, Krystle Leung, Ya-Jen Chang, Rosemarie H DeKruyff, Paul B Savage, Richard Cruse, Christophe Benoit, Dirk Elewaut, Nicole Baumgarth, and Dale T Umetsu. 2019. “A Natural Killer T-cell Subset That Protects Against Airway Hyperreactivity.” Journal of Allergy and Clinical Immunology 143 (2): 565–576.
Chicago author-date (all authors)
Chuang, Ya-Ting, Krystle Leung, Ya-Jen Chang, Rosemarie H DeKruyff, Paul B Savage, Richard Cruse, Christophe Benoit, Dirk Elewaut, Nicole Baumgarth, and Dale T Umetsu. 2019. “A Natural Killer T-cell Subset That Protects Against Airway Hyperreactivity.” Journal of Allergy and Clinical Immunology 143 (2): 565–576.
Vancouver
1.
Chuang Y-T, Leung K, Chang Y-J, DeKruyff RH, Savage PB, Cruse R, et al. A natural killer T-cell subset that protects against airway hyperreactivity. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY. 2019;143(2):565–76.
IEEE
[1]
Y.-T. Chuang et al., “A natural killer T-cell subset that protects against airway hyperreactivity,” JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 143, no. 2, pp. 565–576, 2019.
@article{8605806,
  abstract     = {Background: Infection of suckling mice with influenza virus expands a CD4(-)CD8(-) double-negative (DN) natural killer T (NKT) cell subpopulation that protects the mice as adults against allergen-induced airway hyperreactivity (AHR). However, this NKT cell subset has not been characterized, and the underlying mechanisms of protection remain unknown. 
Objective: We characterized this specific NKT cell subpopulation that developed during influenza infection in neonatal mice and that suppressed the subsequent development of AHR. 
Methods: A cell-surface marker was identified by comparing the mRNA expression profile of wild-type CD4+ NKT cells with that of suppressive V alpha 14 DN NKT cells. The marker-enriched NKT cell subset was then analyzed for its cytokine profile and its suppressive in vitro and in vivo abilities. 
Results: We showed that DN NKT cells with high CD38 expression produced IFN-gamma, but not IL-17, IL-4, or IL-13, and inhibited development of AHR through contact-dependent suppression of helper CD4 T-cell proliferation. The NKT subset expanded in the lungs of neonatal mice after infection with influenza and also after treatment of neonatal mice with Nu-alpha-GalCer, which effectively increased DN CD38(hi) NKT cell numbers. 
Conclusion: These results suggest that early/neonatal exposure to infection or antigen challenge affects subsequent lung immunity by altering the cellular composition of cells in the lung and that some subsets of NKT cells suppress AHR. These results provide a possible mechanism by which prior infections can protect against the development of allergic asthma and might be further explored as a protective measure for young children.},
  author       = {Chuang, Ya-Ting and Leung, Krystle and Chang, Ya-Jen and DeKruyff, Rosemarie H and Savage, Paul B and Cruse, Richard and Benoit, Christophe and Elewaut, Dirk and Baumgarth, Nicole and Umetsu, Dale T},
  issn         = {0091-6749},
  journal      = {JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY},
  keywords     = {NKT CELLS,ALPHA-GALACTOSYLCERAMIDE,BONE-MARROW,EARLY-LIFE,ASTHMA,INNATE,EXPOSURE,LIGAND,ACTIVATION,MICE,Natural killer T subset,asthma,airway hyperreactivity,hygiene,hypothesis,influenza,CD38},
  language     = {eng},
  number       = {2},
  pages        = {565--576},
  title        = {A natural killer T-cell subset that protects against airway hyperreactivity},
  url          = {http://dx.doi.org/10.1016/j.jaci.2018.03.022},
  volume       = {143},
  year         = {2019},
}

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