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Caught green-handed : methods for in vivo detection and visualization of protease activity

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Abstract
Proteases are enzymes that cleave peptide bonds of other proteins. Their omnipresence and diverse activities make them important players in protein homeostasis and turnover of the total cell proteome as well as in signal transduction in plant stress response and development. To fully understand protease function, it is of paramount importance to assess when and where a specific protease is active. Here, we review the existing methods to detect in vivo protease activity by means of imaging chemical activity-based probes and genetically encoded sensors. We focus on the diverse fluorescent and luminescent sensors at the researcher's disposal and evaluate the potential of imaging techniques to deliver in vivo spatiotemporal detail of protease activity. We predict that in the coming years, revised techniques will help to elucidate plant protease activity, functions and hence expand the current status of the field.
Keywords
in vivo imaging, activity-based probes, fluorescent, luminescent, plants, protease, proteolysis, reporter, sensor

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Chicago
Fernández Fernández, Alvaro Daniel, Renier AL van der Hoorn, Kris Gevaert, Frank Van Breusegem, and Simon Stael. 2019. “Caught Green-handed : Methods for in Vivo Detection and Visualization of Protease Activity.” Journal of Experimental Botany.
APA
Fernández Fernández, A. D., van der Hoorn, R. A., Gevaert, K., Van Breusegem, F., & Stael, S. (2019). Caught green-handed : methods for in vivo detection and visualization of protease activity. JOURNAL OF EXPERIMENTAL BOTANY.
Vancouver
1.
Fernández Fernández AD, van der Hoorn RA, Gevaert K, Van Breusegem F, Stael S. Caught green-handed : methods for in vivo detection and visualization of protease activity. JOURNAL OF EXPERIMENTAL BOTANY. 2019;
MLA
Fernández Fernández, Alvaro Daniel et al. “Caught Green-handed : Methods for in Vivo Detection and Visualization of Protease Activity.” JOURNAL OF EXPERIMENTAL BOTANY (2019): n. pag. Print.
@article{8605334,
  abstract     = {Proteases are enzymes that cleave peptide bonds of other proteins. Their omnipresence and diverse activities make them important players in protein homeostasis and turnover of the total cell proteome as well as in signal transduction in plant stress response and development. To fully understand protease function, it is of paramount importance to assess when and where a specific protease is active. Here, we review the existing methods to detect in vivo protease activity by means of imaging chemical activity-based probes and genetically encoded sensors. We focus on the diverse fluorescent and luminescent sensors at the researcher's disposal and evaluate the potential of imaging techniques to deliver in vivo spatiotemporal detail of protease activity. We predict that in the coming years, revised techniques will help to elucidate plant protease activity, functions and hence expand the current status of the field.},
  author       = {Fern{\'a}ndez Fern{\'a}ndez, Alvaro Daniel and van der Hoorn, Renier AL and Gevaert, Kris and Van Breusegem, Frank and Stael, Simon},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  language     = {eng},
  title        = {Caught green-handed : methods for in vivo detection and visualization of protease activity},
  url          = {http://dx.doi.org/10.1093/jxb/erz076},
  year         = {2019},
}

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