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Presence of gammaherpesvirus BoHV-4 in endometrial cytology samples is not associated with subclinical endometritis diagnosed at artificial insemination in dairy cows

Bo Yang (UGent) , Osvaldo Bogado Pascottini, Jiexiong Xie (UGent) , Lowiese Desmarets (UGent) , Tingting Cui (UGent) , Geert Opsomer (UGent) and Hans Nauwynck (UGent)
(2019) Veterinary Microbiology. 229. p.130-137
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Abstract
In the past, bovine herpesvirus 4 (BoHV-4) has been suggested to be associated with metritis and endometritis. However, not many field studies investigated the association between BoHV-4 and subclinical endometritis (SCE). In the present study, the association between the intrauterine presence of BoHV-4 and SCE diagnosed during artificial insemination (AI) was examined on two dairy farms in Belgium. An immunoperoxidase monolayer assay (IPMA) and an enzyme-linked immuno sorbent assay (ELISA) were used to screen the serum for anti-BoHV-4 antibodies. A SYBR green based one step real time qPCR was used to detect and quantify BoHV-4 (ORF20) in nasal, uterine and vaginal samples collected at Al. A reverse transcription qPCR (RT-qPCR) was used to detect mRNA (gB) as proof of a productive BoHV-4 infection. BoHV-4 was detected in 39.4% (farm A)/23.8% (farm B) of the nasal samples, 48.5% (farm A)/19.0% (farm B) of the uterine samples and 51.5% (farm A)/42.9% (farm B) of the vaginal samples. Active replication was only detected in farm A in 38.5% of the BoHV-4 positive nasal samples and in 5.95% positive cases of the vaginal samples. The prevalence of SCE diagnosed at AI was 45.5% and 42.9% in farm A and farm B, respectively. The presence of SCE was associated with a reduced pregnancy outcome at artificial insemination (Al) (P < 0.001). The occurrence of SCE at AI was not associated with the presence of latent or productive BoHV4 infections in the uterus nor in the vagina and nose (P > 0.05).
Keywords
Microbiology, General Veterinary, General Medicine

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Chicago
Yang, Bo, Osvaldo Bogado Pascottini, Jiexiong Xie, Lowiese Desmarets, Tingting Cui, Geert Opsomer, and Hans Nauwynck. 2019. “Presence of Gammaherpesvirus BoHV-4 in Endometrial Cytology Samples Is Not Associated with Subclinical Endometritis Diagnosed at Artificial Insemination in Dairy Cows.” Veterinary Microbiology 229: 130–137.
APA
Yang, B., Pascottini, O. B., Xie, J., Desmarets, L., Cui, T., Opsomer, G., & Nauwynck, H. (2019). Presence of gammaherpesvirus BoHV-4 in endometrial cytology samples is not associated with subclinical endometritis diagnosed at artificial insemination in dairy cows. Veterinary Microbiology, 229, 130–137.
Vancouver
1.
Yang B, Pascottini OB, Xie J, Desmarets L, Cui T, Opsomer G, et al. Presence of gammaherpesvirus BoHV-4 in endometrial cytology samples is not associated with subclinical endometritis diagnosed at artificial insemination in dairy cows. Veterinary Microbiology. Elsevier BV; 2019;229:130–7.
MLA
Yang, Bo et al. “Presence of Gammaherpesvirus BoHV-4 in Endometrial Cytology Samples Is Not Associated with Subclinical Endometritis Diagnosed at Artificial Insemination in Dairy Cows.” Veterinary Microbiology 229 (2019): 130–137. Print.
@article{8605054,
  abstract     = {In the past, bovine herpesvirus 4 (BoHV-4) has been suggested to be associated with metritis and endometritis. However, not many field studies investigated the association between BoHV-4 and subclinical endometritis (SCE). In the present study, the association between the intrauterine presence of BoHV-4 and SCE diagnosed during artificial insemination (AI) was examined on two dairy farms in Belgium. An immunoperoxidase monolayer assay (IPMA) and an enzyme-linked immuno sorbent assay (ELISA) were used to screen the serum for anti-BoHV-4 antibodies. A SYBR green based one step real time qPCR was used to detect and quantify BoHV-4 (ORF20) in nasal, uterine and vaginal samples collected at Al. A reverse transcription qPCR (RT-qPCR) was used to detect mRNA (gB) as proof of a productive BoHV-4 infection. BoHV-4 was detected in 39.4\% (farm A)/23.8\% (farm B) of the nasal samples, 48.5\% (farm A)/19.0\% (farm B) of the uterine samples and 51.5\% (farm A)/42.9\% (farm B) of the vaginal samples. Active replication was only detected in farm A in 38.5\% of the BoHV-4 positive nasal samples and in 5.95\% positive cases of the vaginal samples. The prevalence of SCE diagnosed at AI was 45.5\% and 42.9\% in farm A and farm B, respectively. The presence of SCE was associated with a reduced pregnancy outcome at artificial insemination (Al) (P {\textlangle} 0.001). The occurrence of SCE at AI was not associated with the presence of latent or productive BoHV4 infections in the uterus nor in the vagina and nose (P {\textrangle} 0.05).},
  author       = {Yang, Bo and Pascottini, Osvaldo Bogado and Xie, Jiexiong and Desmarets, Lowiese and Cui, Tingting and Opsomer, Geert and Nauwynck, Hans},
  issn         = {0378-1135},
  journal      = {Veterinary Microbiology},
  language     = {eng},
  pages        = {130--137},
  publisher    = {Elsevier BV},
  title        = {Presence of gammaherpesvirus BoHV-4 in endometrial cytology samples is not associated with subclinical endometritis diagnosed at artificial insemination in dairy cows},
  url          = {http://dx.doi.org/10.1016/j.vetmic.2018.12.025},
  volume       = {229},
  year         = {2019},
}

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