Advanced search
1 file | 739.95 KB

Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23

Author
Organization
Abstract
BACKGROUND: Ov16 serology is considered a reference method for Onchocerca volvulus epidemiological mapping. Given the suboptimal sensitivity of this test and the fact that seroconversion takes more than a year after infection, additional serological tests might be needed to guide onchocerciasis elimination programmes. Recently, two linear epitopes encoded in OvMP-1 and OvMP-23 peptides were introduced as serological markers, but the observed antibody cross-reactivity in samples originating from Onchocerca volvulus non-endemic areas required further investigation. METHODS: We evaluated both peptide markers in an O. volvulus hypo-endemic setting in Jimma Town, Ethiopia using peptide ELISA. For all individuals (n = 303), the infection status with soil-transmitted helminths and Schistosoma mansoni was known. RESULTS: We found that 11 (3.6%) individuals were positive for anti-Ov16 IgG4 antibodies, while 34 (11.2%) and 15 (5.0%) individuals were positive for OvMP-1 and OvMP-23, respectively. Out of the 34 OvMP-1 positive samples, 33 were negative on the Ov16 IgG4 ELISA. Similarly, out of the 15 OvMP-23 positive samples, 14 scored negative on this reference method. No difference in seroprevalence for all three markers could be observed between uninfected individuals and individuals infected with different soil-transmitted helminths or S. mansoni. Moreover, the intensity of the response to OvMP-1, OvMP-23 or Ov16 was not significantly stronger in individuals carrying patent STH or S. mansoni infections, nor was there any correlation between the intensities of the responses to the three different antigens. CONCLUSIONS: This study demonstrates that a patent infection with either soil-transmitted helminths or S. mansoni does not lead to increased antibody recognition of both OvMP-1 and OvMP23.
Keywords
Parasitology, Infectious Diseases

Downloads

  • document-1.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 739.95 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Vlaminck, Johnny, Ole Lagatie, Ann Verheyen, Daniel Dana, Bieke Van Dorst, Zeleke Mekonnen Kurmane, Bruno Levecke, and Lieven J. Stuyver. 2019. “Patent Infections with Soil-transmitted Helminths and Schistosoma Mansoni Are Not Associated with Increased Prevalence of Antibodies to the Onchocerca Volvulus Peptide Epitopes OvMP-1 and OvMP-23.” Parasites & Vectors 12 (1).
APA
Vlaminck, J., Lagatie, O., Verheyen, A., Dana, D., Van Dorst, B., Mekonnen Kurmane, Z., Levecke, B., et al. (2019). Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23. Parasites & Vectors, 12(1).
Vancouver
1.
Vlaminck J, Lagatie O, Verheyen A, Dana D, Van Dorst B, Mekonnen Kurmane Z, et al. Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23. Parasites & Vectors. Springer Nature; 2019;12(1).
MLA
Vlaminck, Johnny et al. “Patent Infections with Soil-transmitted Helminths and Schistosoma Mansoni Are Not Associated with Increased Prevalence of Antibodies to the Onchocerca Volvulus Peptide Epitopes OvMP-1 and OvMP-23.” Parasites & Vectors 12.1 (2019): n. pag. Print.
@article{8604609,
  abstract     = {BACKGROUND:

Ov16 serology is considered a reference method for Onchocerca volvulus epidemiological mapping. Given the suboptimal sensitivity of this test and the fact that seroconversion takes more than a year after infection, additional serological tests might be needed to guide onchocerciasis elimination programmes. Recently, two linear epitopes encoded in OvMP-1 and OvMP-23 peptides were introduced as serological markers, but the observed antibody cross-reactivity in samples originating from Onchocerca volvulus non-endemic areas required further investigation.
METHODS:

We evaluated both peptide markers in an O. volvulus hypo-endemic setting in Jimma Town, Ethiopia using peptide ELISA. For all individuals (n = 303), the infection status with soil-transmitted helminths and Schistosoma mansoni was known.
RESULTS:

We found that 11 (3.6\%) individuals were positive for anti-Ov16 IgG4 antibodies, while 34 (11.2\%) and 15 (5.0\%) individuals were positive for OvMP-1 and OvMP-23, respectively. Out of the 34 OvMP-1 positive samples, 33 were negative on the Ov16 IgG4 ELISA. Similarly, out of the 15 OvMP-23 positive samples, 14 scored negative on this reference method. No difference in seroprevalence for all three markers could be observed between uninfected individuals and individuals infected with different soil-transmitted helminths or S. mansoni. Moreover, the intensity of the response to OvMP-1, OvMP-23 or Ov16 was not significantly stronger in individuals carrying patent STH or S. mansoni infections, nor was there any correlation between the intensities of the responses to the three different antigens.
CONCLUSIONS:

This study demonstrates that a patent infection with either soil-transmitted helminths or S. mansoni does not lead to increased antibody recognition of both OvMP-1 and OvMP23.},
  author       = {Vlaminck, Johnny and Lagatie, Ole and Verheyen, Ann and Dana, Daniel and Van Dorst, Bieke and Mekonnen Kurmane, Zeleke and Levecke, Bruno and Stuyver, Lieven J.},
  issn         = {1756-3305},
  journal      = {Parasites \& Vectors},
  number       = {1},
  publisher    = {Springer Nature},
  title        = {Patent infections with soil-transmitted helminths and Schistosoma mansoni are not associated with increased prevalence of antibodies to the Onchocerca volvulus peptide epitopes OvMP-1 and OvMP-23},
  url          = {http://dx.doi.org/10.1186/s13071-019-3308-z},
  volume       = {12},
  year         = {2019},
}

Altmetric
View in Altmetric