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Bacillus velezensis as antagonist towards Penicillium roqueforti s.l. in silage : in vitro and in vivo evaluation

Eva Wambacq (UGent) , Kris Audenaert (UGent) , Monica Höfte (UGent) , Sarah De Saeger (UGent) and Geert Haesaert (UGent)
(2018) JOURNAL OF APPLIED MICROBIOLOGY. 125(4). p.986-996
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Abstract
Aims: The present study was conducted to evaluate the antagonistic effect of BacillusvelezensisNRRL B-23189 towards Penicillium roqueforti s.s. and Penicillium paneum (designated together as P.roqueforti s.l.) in silage conditions. Methods and Results: Corn silage conditions were simulated invitro, and the impact of B.velezensis culture supernatant or cell suspension on P.roqueforti s.l. growth and roquefortine C production was evaluated. The antagonism was promising, but growth of B.velezensis in corn silage infusion was poor. Additionally, an invivo experiment was carried out with mini-silos containing a mixture of perennial ryegrass and white clover inoculated with P.roquefortis.l. The applied B.velezensis cell suspension was unsuccessful in reducing P.roquefortis.l. numbers, but did not compromise the silage acidification. Conclusions: Although the antagonism observed invitro was promising, the applied B.velezensis cell suspension could not live up to the expectations invivo. Significance and Impact of the Study: To our knowledge, the present study is the first one evaluating the antagonistic properties of B.velezensis towards toxigenic moulds in silage conditions, offering a good base for further research.
Keywords
LACTIC-ACID BACTERIA, BALED GRASS-SILAGE, ANIMAL FEED, TOXIGENIC FUNGI, PICHIA-ANOMALA, CORN-SILAGE, MYCOTOXINS, SUBTILIS, GROWTH, BIOCONTROL, Bacillus, biocontrol, fermentation, mycotoxins, Penicillium

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MLA
Wambacq, Eva, et al. “Bacillus Velezensis as Antagonist towards Penicillium Roqueforti s.l. in Silage : In Vitro and in Vivo Evaluation.” JOURNAL OF APPLIED MICROBIOLOGY, vol. 125, no. 4, 2018, pp. 986–96, doi:10.1111/jam.13944.
APA
Wambacq, E., Audenaert, K., Höfte, M., De Saeger, S., & Haesaert, G. (2018). Bacillus velezensis as antagonist towards Penicillium roqueforti s.l. in silage : in vitro and in vivo evaluation. JOURNAL OF APPLIED MICROBIOLOGY, 125(4), 986–996. https://doi.org/10.1111/jam.13944
Chicago author-date
Wambacq, Eva, Kris Audenaert, Monica Höfte, Sarah De Saeger, and Geert Haesaert. 2018. “Bacillus Velezensis as Antagonist towards Penicillium Roqueforti s.l. in Silage : In Vitro and in Vivo Evaluation.” JOURNAL OF APPLIED MICROBIOLOGY 125 (4): 986–96. https://doi.org/10.1111/jam.13944.
Chicago author-date (all authors)
Wambacq, Eva, Kris Audenaert, Monica Höfte, Sarah De Saeger, and Geert Haesaert. 2018. “Bacillus Velezensis as Antagonist towards Penicillium Roqueforti s.l. in Silage : In Vitro and in Vivo Evaluation.” JOURNAL OF APPLIED MICROBIOLOGY 125 (4): 986–996. doi:10.1111/jam.13944.
Vancouver
1.
Wambacq E, Audenaert K, Höfte M, De Saeger S, Haesaert G. Bacillus velezensis as antagonist towards Penicillium roqueforti s.l. in silage : in vitro and in vivo evaluation. JOURNAL OF APPLIED MICROBIOLOGY. 2018;125(4):986–96.
IEEE
[1]
E. Wambacq, K. Audenaert, M. Höfte, S. De Saeger, and G. Haesaert, “Bacillus velezensis as antagonist towards Penicillium roqueforti s.l. in silage : in vitro and in vivo evaluation,” JOURNAL OF APPLIED MICROBIOLOGY, vol. 125, no. 4, pp. 986–996, 2018.
@article{8604448,
  abstract     = {{Aims: The present study was conducted to evaluate the antagonistic effect of BacillusvelezensisNRRL B-23189 towards Penicillium roqueforti s.s. and Penicillium paneum (designated together as P.roqueforti s.l.) in silage conditions. 
Methods and Results: Corn silage conditions were simulated invitro, and the impact of B.velezensis culture supernatant or cell suspension on P.roqueforti s.l. growth and roquefortine C production was evaluated. The antagonism was promising, but growth of B.velezensis in corn silage infusion was poor. Additionally, an invivo experiment was carried out with mini-silos containing a mixture of perennial ryegrass and white clover inoculated with P.roquefortis.l. The applied B.velezensis cell suspension was unsuccessful in reducing P.roquefortis.l. numbers, but did not compromise the silage acidification. 
Conclusions: Although the antagonism observed invitro was promising, the applied B.velezensis cell suspension could not live up to the expectations invivo. 
Significance and Impact of the Study: To our knowledge, the present study is the first one evaluating the antagonistic properties of B.velezensis towards toxigenic moulds in silage conditions, offering a good base for further research.}},
  author       = {{Wambacq, Eva and Audenaert, Kris and Höfte, Monica and De Saeger, Sarah and Haesaert, Geert}},
  issn         = {{1364-5072}},
  journal      = {{JOURNAL OF APPLIED MICROBIOLOGY}},
  keywords     = {{LACTIC-ACID BACTERIA,BALED GRASS-SILAGE,ANIMAL FEED,TOXIGENIC FUNGI,PICHIA-ANOMALA,CORN-SILAGE,MYCOTOXINS,SUBTILIS,GROWTH,BIOCONTROL,Bacillus,biocontrol,fermentation,mycotoxins,Penicillium}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{986--996}},
  title        = {{Bacillus velezensis as antagonist towards Penicillium roqueforti s.l. in silage : in vitro and in vivo evaluation}},
  url          = {{http://dx.doi.org/10.1111/jam.13944}},
  volume       = {{125}},
  year         = {{2018}},
}

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