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An engineered combinatorial module of transcription factors boosts production of monoterpenoid indole alkaloids in Catharanthus roseus

(2018) METABOLIC ENGINEERING. 48. p.150-162
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Abstract
To fend off microbial pathogens and herbivores, plants have evolved a wide range of defense strategies such as physical barriers, or the production of anti-digestive proteins or bioactive specialized metabolites. Accumulation of the latter compounds is often regulated by transcriptional activation of the biosynthesis pathway genes by the phytohormone jasmonate-isoleucine. Here, we used our recently developed flower petal transformation method in the medicinal plant Catharanthus roseus to shed light on the complex regulatory mechanisms steering the jasmonate-modulated biosynthesis of monoterpenoid indole alkaloids (MIAs), to which the anti-cancer compounds vinblastine and vincristine belong. By combinatorial overexpression of the transcriptional activators BIS1, ORCA3 and MYC2a, we provide an unprecedented insight into the modular transcriptional control of MIA biosynthesis. Furthermore, we show that the expression of an engineered de-repressed MYC2a triggers a tremendous reprogramming of the MIA pathway, finally leading to massively increased accumulation of at least 23 MIAs. The current study unveils an innovative approach for future metabolic engineering efforts for the production of valuable bioactive plant compounds in non-model plants.
Keywords
Madagascar periwinkle, Jasmonate, Metabolic engineering, Monoterpenoid indole alkaloids, Transcription factor, MYC2, PLANT SECONDARY METABOLISM, BIOSYNTHETIC GENE STR, MADAGASCAR PERIWINKLE, SIGNAL-TRANSDUCTION, 7-DEOXYLOGANETIC ACID, IRIDOID BIOSYNTHESIS, RESPONSIVE ELEMENT, MOLECULAR-CLONING, JAZ REPRESSION, JASMONIC ACID

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Chicago
Schweizer, Fabian, Maite Colinas Martinez, Jacob Pollier, Alex Van Moerkercke, Robin Vanden Bossche, Rebecca De Clercq, and Alain Goossens. 2018. “An Engineered Combinatorial Module of Transcription Factors Boosts Production of Monoterpenoid Indole Alkaloids in Catharanthus Roseus.” Metabolic Engineering 48: 150–162.
APA
Schweizer, F., Colinas Martinez, M., Pollier, J., Van Moerkercke, A., Vanden Bossche, R., De Clercq, R., & Goossens, A. (2018). An engineered combinatorial module of transcription factors boosts production of monoterpenoid indole alkaloids in Catharanthus roseus. METABOLIC ENGINEERING, 48, 150–162.
Vancouver
1.
Schweizer F, Colinas Martinez M, Pollier J, Van Moerkercke A, Vanden Bossche R, De Clercq R, et al. An engineered combinatorial module of transcription factors boosts production of monoterpenoid indole alkaloids in Catharanthus roseus. METABOLIC ENGINEERING. 2018;48:150–62.
MLA
Schweizer, Fabian et al. “An Engineered Combinatorial Module of Transcription Factors Boosts Production of Monoterpenoid Indole Alkaloids in Catharanthus Roseus.” METABOLIC ENGINEERING 48 (2018): 150–162. Print.
@article{8604202,
  abstract     = {To fend off microbial pathogens and herbivores, plants have evolved a wide range of defense strategies such as physical barriers, or the production of anti-digestive proteins or bioactive specialized metabolites. Accumulation of the latter compounds is often regulated by transcriptional activation of the biosynthesis pathway genes by the phytohormone jasmonate-isoleucine. Here, we used our recently developed flower petal transformation method in the medicinal plant Catharanthus roseus to shed light on the complex regulatory mechanisms steering the jasmonate-modulated biosynthesis of monoterpenoid indole alkaloids (MIAs), to which the anti-cancer compounds vinblastine and vincristine belong. By combinatorial overexpression of the transcriptional activators BIS1, ORCA3 and MYC2a, we provide an unprecedented insight into the modular transcriptional control of MIA biosynthesis. Furthermore, we show that the expression of an engineered de-repressed MYC2a triggers a tremendous reprogramming of the MIA pathway, finally leading to massively increased accumulation of at least 23 MIAs. The current study unveils an innovative approach for future metabolic engineering efforts for the production of valuable bioactive plant compounds in non-model plants.},
  author       = {Schweizer, Fabian and Colinas Martinez, Maite and Pollier, Jacob and Van Moerkercke, Alex and Vanden Bossche, Robin and De Clercq, Rebecca and Goossens, Alain},
  issn         = {1096-7176},
  journal      = {METABOLIC ENGINEERING},
  language     = {eng},
  pages        = {150--162},
  title        = {An engineered combinatorial module of transcription factors boosts production of monoterpenoid indole alkaloids in Catharanthus roseus},
  url          = {http://dx.doi.org/10.1016/j.ymben.2018.05.016},
  volume       = {48},
  year         = {2018},
}

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