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Comprehensive mapping of the antigenic peptide repertoire during bacterial infection

Rupert Mayer (UGent) , Teresa Maia (UGent) , Katie Boucher (UGent) , Bart Vandekerckhove (UGent) and Francis Impens (UGent)
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Abstract
Antibacterial vaccines are increasingly recognized as highly effective tools to mitigate antibiotics resistance. For the rational design of novel vaccines, knowledge about the identity of antigenic bacterial peptides presented on infected cells is essential, however, this is information is incomplete or lacking for many bacteria. Hence, this project aims to i) establish a workflow for the identification of bacterial antigenic peptides presented on infected cells by mass spectrometry (MS) and ii) map the repertoire of peptides presented during bacterial infection with Listeria and Salmonella, two major types of human-pathogenic model bacteria. The project focuses on harvesting and enriching peptides presented on host cells by MHC class I followed by MS-based peptide detection. Finally, bioinformatic tools like MaxQuant and de novo sequencing algorithms will aid in peptide sequence identification.

Citation

Please use this url to cite or link to this publication:

Chicago
Mayer, Rupert, Teresa Maia, Katie Boucher, Bart Vandekerckhove, and Francis Impens. 2018. “Comprehensive Mapping of the Antigenic Peptide Repertoire During Bacterial Infection.” In Belgian Proteomics Association, Conference Abstracts.
APA
Mayer, R., Maia, T., Boucher, K., Vandekerckhove, B., & Impens, F. (2018). Comprehensive mapping of the antigenic peptide repertoire during bacterial infection. Belgian Proteomics Association, Conference abstracts. Presented at the Belgian Proteomics Association conference 2018 (BePAc 2018).
Vancouver
1.
Mayer R, Maia T, Boucher K, Vandekerckhove B, Impens F. Comprehensive mapping of the antigenic peptide repertoire during bacterial infection. Belgian Proteomics Association, Conference abstracts. 2018.
MLA
Mayer, Rupert et al. “Comprehensive Mapping of the Antigenic Peptide Repertoire During Bacterial Infection.” Belgian Proteomics Association, Conference Abstracts. 2018. Print.
@inproceedings{8603055,
  abstract     = {Antibacterial vaccines are increasingly recognized as highly effective tools to mitigate antibiotics resistance. For the rational design of novel vaccines, knowledge about the identity of antigenic bacterial peptides presented on infected cells is essential, however, this is information is incomplete or lacking for many bacteria. Hence, this project aims to i) establish a workflow for the identification of bacterial antigenic peptides presented on infected cells by mass spectrometry (MS) and ii) map the repertoire of peptides presented during bacterial infection with Listeria and Salmonella, two major types of human-pathogenic model bacteria. The project focuses on harvesting and enriching peptides presented on host cells by MHC class I followed by MS-based peptide detection. Finally, bioinformatic tools like MaxQuant and de novo sequencing algorithms will aid in peptide sequence identification.},
  author       = {Mayer, Rupert and Maia, Teresa and Boucher, Katie and Vandekerckhove, Bart and Impens, Francis},
  booktitle    = {Belgian Proteomics Association, Conference abstracts},
  location     = {Brussels, Belgium},
  title        = {Comprehensive mapping of the antigenic peptide repertoire during bacterial infection},
  year         = {2018},
}