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A Meloidogyne graminicola C-type lectin, Mg01965, is secreted into the host apoplast to suppress plant defence and promote parasitism

Kan Zhuo (UGent) , Diana Naalden (UGent) , Silke Nowak (UGent) , Nguyen Xuan Huy, Lander Bauters (UGent) and Godelieve Gheysen (UGent)
(2019) MOLECULAR PLANT PATHOLOGY. 20(3). p.346-355
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Abstract
C-type lectins (CTLs), a class of multifunctional proteins, are numerous in nematodes. One CTL gene, Mg01965, shown to be expressed in the subventral glands, especially in the second-stage juveniles of the root-knot nematode Meloidogyne graminicola, was further analysed in this study. In vitro RNA interference targeting Mg01965 in the preparasitic juveniles significantly reduced their ability to infect host plant roots. Immunolocalizations showed that Mg01965 is secreted by M. graminicola into the roots during the early parasitic stages and accumulates in the apoplast. Transient expression of Mg01965 in Nicotiana benthamiana and targeting it to the apoplast suppressed the burst of reactive oxygen species triggered by flg22. The CTL Mg01965 suppresses plant innate immunity in the host apoplast, promoting nematode parasitism in the early infection stages.
Keywords
apoplast, C-type lectin, effector, Meloidogyne graminicola, PTI suppression, RNAi, ROOT-KNOT NEMATODE, DISEASE RESISTANCE, RICE, EFFECTOR, PROTEINS, IDENTIFICATION, TRANSCRIPTOME, CALRETICULIN, ARABIDOPSIS, PERCEPTION

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MLA
Zhuo, Kan, et al. “A Meloidogyne Graminicola C-Type Lectin, Mg01965, Is Secreted into the Host Apoplast to Suppress Plant Defence and Promote Parasitism.” MOLECULAR PLANT PATHOLOGY, vol. 20, no. 3, 2019, pp. 346–55.
APA
Zhuo, K., Naalden, D., Nowak, S., Xuan Huy, N., Bauters, L., & Gheysen, G. (2019). A Meloidogyne graminicola C-type lectin, Mg01965, is secreted into the host apoplast to suppress plant defence and promote parasitism. MOLECULAR PLANT PATHOLOGY, 20(3), 346–355.
Chicago author-date
Zhuo, Kan, Diana Naalden, Silke Nowak, Nguyen Xuan Huy, Lander Bauters, and Godelieve Gheysen. 2019. “A Meloidogyne Graminicola C-Type Lectin, Mg01965, Is Secreted into the Host Apoplast to Suppress Plant Defence and Promote Parasitism.” MOLECULAR PLANT PATHOLOGY 20 (3): 346–55.
Chicago author-date (all authors)
Zhuo, Kan, Diana Naalden, Silke Nowak, Nguyen Xuan Huy, Lander Bauters, and Godelieve Gheysen. 2019. “A Meloidogyne Graminicola C-Type Lectin, Mg01965, Is Secreted into the Host Apoplast to Suppress Plant Defence and Promote Parasitism.” MOLECULAR PLANT PATHOLOGY 20 (3): 346–355.
Vancouver
1.
Zhuo K, Naalden D, Nowak S, Xuan Huy N, Bauters L, Gheysen G. A Meloidogyne graminicola C-type lectin, Mg01965, is secreted into the host apoplast to suppress plant defence and promote parasitism. MOLECULAR PLANT PATHOLOGY. 2019;20(3):346–55.
IEEE
[1]
K. Zhuo, D. Naalden, S. Nowak, N. Xuan Huy, L. Bauters, and G. Gheysen, “A Meloidogyne graminicola C-type lectin, Mg01965, is secreted into the host apoplast to suppress plant defence and promote parasitism,” MOLECULAR PLANT PATHOLOGY, vol. 20, no. 3, pp. 346–355, 2019.
@article{8602878,
  abstract     = {C-type lectins (CTLs), a class of multifunctional proteins, are numerous in nematodes. One CTL gene, Mg01965, shown to be expressed in the subventral glands, especially in the second-stage juveniles of the root-knot nematode Meloidogyne graminicola, was further analysed in this study. In vitro RNA interference targeting Mg01965 in the preparasitic juveniles significantly reduced their ability to infect host plant roots. Immunolocalizations showed that Mg01965 is secreted by M. graminicola into the roots during the early parasitic stages and accumulates in the apoplast. Transient expression of Mg01965 in Nicotiana benthamiana and targeting it to the apoplast suppressed the burst of reactive oxygen species triggered by flg22. The CTL Mg01965 suppresses plant innate immunity in the host apoplast, promoting nematode parasitism in the early infection stages.},
  author       = {Zhuo, Kan and Naalden, Diana and Nowak, Silke and Xuan Huy, Nguyen and Bauters, Lander and Gheysen, Godelieve},
  issn         = {1464-6722},
  journal      = {MOLECULAR PLANT PATHOLOGY},
  keywords     = {apoplast,C-type lectin,effector,Meloidogyne graminicola,PTI suppression,RNAi,ROOT-KNOT NEMATODE,DISEASE RESISTANCE,RICE,EFFECTOR,PROTEINS,IDENTIFICATION,TRANSCRIPTOME,CALRETICULIN,ARABIDOPSIS,PERCEPTION},
  language     = {eng},
  number       = {3},
  pages        = {346--355},
  title        = {A Meloidogyne graminicola C-type lectin, Mg01965, is secreted into the host apoplast to suppress plant defence and promote parasitism},
  url          = {http://dx.doi.org/10.1111/mpp.12759},
  volume       = {20},
  year         = {2019},
}

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