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Establishing the diagnosis of chronic colonization with Pseudomonas aeruginosa of cystic fibrosis patients: Comparison of the European consensus criteria with genotyping of P-aeruginosa isolates

(2018) JOURNAL OF CYSTIC FIBROSIS. 17(6). p.729-735
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Abstract
After antibiotic eradication treatment for a first ever Pseudomonas aeruginosa isolation, the European consensus criteria (ECC) are widely used to assess colonization status with P. aeruginosa in CF-patients. We evaluated to what extent genotyping (GT) of subsequent P. aeruginosa isolates could predict/assess chronic colonization (CC), in comparison with the ECC. Methods: Over a 14-year period, sputa were cultured from 80 CF-patients (age range: 2-51 years), from a first ever isolation of P. aeruginosa onwards. Patients with a positive culture for P. aeruginosa received antibiotic eradication treatment. For the 40 patients for whom three or more P. aeruginosa isolates were available, these isolates were genotyped. Results: According to the ECC, 27 out of the 40 patients (67.5%) became CC during the study period (ECC-positive patients). Genotyping confirmed persistence of the same genotype for 25 of these ECC-positive patients. Genotyping indicated persistence of the same genotype for at least two subsequent isolates for 5 out of 13 ECC-negative patients. Culture-positivity characteristics of the 27 ECC-positive patients corresponded well to those of the 30 GT-positive patients, with an overall higher number of positive cultures as well as a shorter interval in between first and second isolate compared to ECC-negative and GT-negative patients. Genotyping indicated persistence of the same genotype on average 9.3 months earlier than CC according to the ECC (P < 0.01). Conclusions: Genotyping of P. aeruginosa isolates confirmed CC for 25 out of 27 ECC-positive patients (92.6% specificity) and predicted CC 9.3 months earlier than the ECC. (C) 2018 European Cystic Fibrosis Society. Published by 'Elsevier B.V. All rights reserved.
Keywords
CHRONIC LUNG INFECTION, ERADICATION THERAPY, ANTIBODIES, ADAPTATION, SURVIVAL, STRAINS, Cystic fibrosis, Pseudomonas aeruginosa, European consensus criteria, (ECC), Genotyping (GT), McRAPD

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Chicago
Jonckheere, Leander, Petra Schelstraete, Leen Van Simaey, Eva Van Braeckel, Julie Willekens, Van daeleSabine, Frans De Baets, and Mario Vaneechoutte. 2018. “Establishing the Diagnosis of Chronic Colonization with Pseudomonas Aeruginosa of Cystic Fibrosis Patients: Comparison of the European Consensus Criteria with Genotyping of P-aeruginosa Isolates.” Journal of Cystic Fibrosis 17 (6): 729–735.
APA
Jonckheere, L., Schelstraete, P., Van Simaey, L., Van Braeckel, E., Willekens, J., Van daeleSabine, De Baets, F., et al. (2018). Establishing the diagnosis of chronic colonization with Pseudomonas aeruginosa of cystic fibrosis patients: Comparison of the European consensus criteria with genotyping of P-aeruginosa isolates. JOURNAL OF CYSTIC FIBROSIS, 17(6), 729–735.
Vancouver
1.
Jonckheere L, Schelstraete P, Van Simaey L, Van Braeckel E, Willekens J, Van daeleSabine, et al. Establishing the diagnosis of chronic colonization with Pseudomonas aeruginosa of cystic fibrosis patients: Comparison of the European consensus criteria with genotyping of P-aeruginosa isolates. JOURNAL OF CYSTIC FIBROSIS. Amsterdam: Elsevier Science Bv; 2018;17(6):729–35.
MLA
Jonckheere, Leander et al. “Establishing the Diagnosis of Chronic Colonization with Pseudomonas Aeruginosa of Cystic Fibrosis Patients: Comparison of the European Consensus Criteria with Genotyping of P-aeruginosa Isolates.” JOURNAL OF CYSTIC FIBROSIS 17.6 (2018): 729–735. Print.
@article{8601280,
  abstract     = {After antibiotic eradication treatment for a first ever Pseudomonas aeruginosa isolation, the European consensus criteria (ECC) are widely used to assess colonization status with P. aeruginosa in CF-patients. We evaluated to what extent genotyping (GT) of subsequent P. aeruginosa isolates could predict/assess chronic colonization (CC), in comparison with the ECC. Methods: Over a 14-year period, sputa were cultured from 80 CF-patients (age range: 2-51 years), from a first ever isolation of P. aeruginosa onwards. Patients with a positive culture for P. aeruginosa received antibiotic eradication treatment. For the 40 patients for whom three or more P. aeruginosa isolates were available, these isolates were genotyped. Results: According to the ECC, 27 out of the 40 patients (67.5\%) became CC during the study period (ECC-positive patients). Genotyping confirmed persistence of the same genotype for 25 of these ECC-positive patients. Genotyping indicated persistence of the same genotype for at least two subsequent isolates for 5 out of 13 ECC-negative patients. Culture-positivity characteristics of the 27 ECC-positive patients corresponded well to those of the 30 GT-positive patients, with an overall higher number of positive cultures as well as a shorter interval in between first and second isolate compared to ECC-negative and GT-negative patients. Genotyping indicated persistence of the same genotype on average 9.3 months earlier than CC according to the ECC (P {\textlangle} 0.01). Conclusions: Genotyping of P. aeruginosa isolates confirmed CC for 25 out of 27 ECC-positive patients (92.6\% specificity) and predicted CC 9.3 months earlier than the ECC. (C) 2018 European Cystic Fibrosis Society. Published by 'Elsevier B.V. All rights reserved.},
  author       = {Jonckheere, Leander and Schelstraete, Petra and Van Simaey, Leen and Van Braeckel, Eva and Willekens, Julie and Van daele, Sabine and De Baets, Frans and Vaneechoutte, Mario},
  issn         = {1569-1993},
  journal      = {JOURNAL OF CYSTIC FIBROSIS},
  language     = {eng},
  number       = {6},
  pages        = {729--735},
  publisher    = {Elsevier Science Bv},
  title        = {Establishing the diagnosis of chronic colonization with Pseudomonas aeruginosa of cystic fibrosis patients: Comparison of the European consensus criteria with genotyping of P-aeruginosa isolates},
  url          = {http://dx.doi.org/10.1016/j.jcf.2018.03.004},
  volume       = {17},
  year         = {2018},
}

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