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Anidulafungin increases the antibacterial activity of tigecycline in polymicrobial Candida albicans/Staphylococcus aureus biofilms on intraperitoneally innplanted foreign bodies

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Abstract
Objectives: We aimed to establish a novel murine intra-abdominal foreign body infection model to study the activity of anidulafung in and tigecycline against dual species Candida albicans/Staphylococcus aureus biofirrns. Methods: In vitro and in vivo single and dual species biofilms were developed inside serum-coated triple-lumen catheters placed in 24-well plates or implanted intraperitoneally in BALB/c mice. The effect of tigecycline and anidulafungin alone and in combination was tested using clinically relevant concentrations. Scanning electron microscopy was used to visualize the mature biofilm structure developed intraperitoneally. Flow cytometry was used to determine the immunological response upon infection, Immunoblot analysis allowed us to determine the effect of anidulafungin on poly-beta-(1,6)-N-acetylglucosamine in in vitro-grown S. aureus biofirms. Results: We determined the MIC, MBC and in vitro susceptibility profile for anidulafungin and tigecycline against C. albicans and S. aureus in mixed and single species biofilms. We demonstrated that a nidulafungin acts synergistically when combined with tigecycline against in vivo intra-abdominal biofilms, Moreover, we reveal that a nidulafungin reduces the abundance of S. aureus poly-beta-(1,6)-N-acetylglucosamine. The influx of neutrophils is much increased when infected with mixed biofilms compared with single species biofilms. Conclusions: Currently, treatment of intra-abdominal infections, in particular polymicrobial catheter-associated peritonitis, is ineffective, To the best of our knowledge, this is the first study that provides insight into new possible options for treatment of C. albicans/S. aureus biofilms present in the abdominal cavity.
Keywords
ALBICANS BIOFILMS, INFECTION, GLYCYLCYCLINES, PERITONITIS, STABILITY, EFFICACY, MODEL

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MLA
Rogiers, Ona et al. “Anidulafungin Increases the Antibacterial Activity of Tigecycline in Polymicrobial Candida albicans/Staphylococcus Aureus Biofilms on Intraperitoneally Innplanted Foreign Bodies.” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 73.10 (2018): 2806–2814. Print.
APA
Rogiers, O., Holtappels, M., Siala, W., Lamkanfi, M., Van Bambeke, F., Lagrou, K., Van Dijck, P., et al. (2018). Anidulafungin increases the antibacterial activity of tigecycline in polymicrobial Candida albicans/Staphylococcus aureus biofilms on intraperitoneally innplanted foreign bodies. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 73(10), 2806–2814.
Chicago author-date
Rogiers, Ona, Michelle Holtappels, Wafi Siala, Mohamed Lamkanfi, Francoise Van Bambeke, Katrien Lagrou, Patrick Van Dijck, and Sona Kucharikova. 2018. “Anidulafungin Increases the Antibacterial Activity of Tigecycline in Polymicrobial Candida albicans/Staphylococcus Aureus Biofilms on Intraperitoneally Innplanted Foreign Bodies.” Journal of Antimicrobial Chemotherapy 73 (10): 2806–2814.
Chicago author-date (all authors)
Rogiers, Ona, Michelle Holtappels, Wafi Siala, Mohamed Lamkanfi, Francoise Van Bambeke, Katrien Lagrou, Patrick Van Dijck, and Sona Kucharikova. 2018. “Anidulafungin Increases the Antibacterial Activity of Tigecycline in Polymicrobial Candida albicans/Staphylococcus Aureus Biofilms on Intraperitoneally Innplanted Foreign Bodies.” Journal of Antimicrobial Chemotherapy 73 (10): 2806–2814.
Vancouver
1.
Rogiers O, Holtappels M, Siala W, Lamkanfi M, Van Bambeke F, Lagrou K, et al. Anidulafungin increases the antibacterial activity of tigecycline in polymicrobial Candida albicans/Staphylococcus aureus biofilms on intraperitoneally innplanted foreign bodies. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 2018;73(10):2806–14.
IEEE
[1]
O. Rogiers et al., “Anidulafungin increases the antibacterial activity of tigecycline in polymicrobial Candida albicans/Staphylococcus aureus biofilms on intraperitoneally innplanted foreign bodies,” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, vol. 73, no. 10, pp. 2806–2814, 2018.
@article{8601019,
  abstract     = {Objectives: We aimed to establish a novel murine intra-abdominal foreign body infection model to study the activity of anidulafung in and tigecycline against dual species Candida albicans/Staphylococcus aureus biofirrns. 
Methods: In vitro and in vivo single and dual species biofilms were developed inside serum-coated triple-lumen catheters placed in 24-well plates or implanted intraperitoneally in BALB/c mice. The effect of tigecycline and anidulafungin alone and in combination was tested using clinically relevant concentrations. Scanning electron microscopy was used to visualize the mature biofilm structure developed intraperitoneally. Flow cytometry was used to determine the immunological response upon infection, Immunoblot analysis allowed us to determine the effect of anidulafungin on poly-beta-(1,6)-N-acetylglucosamine in in vitro-grown S. aureus biofirms. 
Results: We determined the MIC, MBC and in vitro susceptibility profile for anidulafungin and tigecycline against C. albicans and S. aureus in mixed and single species biofilms. We demonstrated that a nidulafungin acts synergistically when combined with tigecycline against in vivo intra-abdominal biofilms, Moreover, we reveal that a nidulafungin reduces the abundance of S. aureus poly-beta-(1,6)-N-acetylglucosamine. The influx of neutrophils is much increased when infected with mixed biofilms compared with single species biofilms. 
Conclusions: Currently, treatment of intra-abdominal infections, in particular polymicrobial catheter-associated peritonitis, is ineffective, To the best of our knowledge, this is the first study that provides insight into new possible options for treatment of C. albicans/S. aureus biofilms present in the abdominal cavity.},
  author       = {Rogiers, Ona and Holtappels, Michelle and Siala, Wafi and Lamkanfi, Mohamed and Van Bambeke, Francoise and Lagrou, Katrien and Van Dijck, Patrick and Kucharikova, Sona},
  issn         = {0305-7453},
  journal      = {JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY},
  keywords     = {ALBICANS BIOFILMS,INFECTION,GLYCYLCYCLINES,PERITONITIS,STABILITY,EFFICACY,MODEL},
  language     = {eng},
  number       = {10},
  pages        = {2806--2814},
  title        = {Anidulafungin increases the antibacterial activity of tigecycline in polymicrobial Candida albicans/Staphylococcus aureus biofilms on intraperitoneally innplanted foreign bodies},
  url          = {http://dx.doi.org/10.1093/jac/dky246},
  volume       = {73},
  year         = {2018},
}

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