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The presence of extracellular microRNAs in the media of cultured Drosophila cells

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Abstract
While regulatory RNA pathways, such as RNAi, have commonly been described at an intracellular level, studies investigating extracellular RNA species in insects are lacking. In the present study, we demonstrate the presence of extracellular microRNAs (miRNAs) in the cell-free conditioned media of two Drosophila cell lines. More specifically, by means of quantitative real-time PCR (qRT-PCR), we analysed the presence of twelve miRNAs in extracellular vesicles (EVs) and in extracellular Argonaute-1 containing immunoprecipitates, obtained from the cell-free conditioned media of S2 and Cl. 8 cell cultures. Next-generation RNA-sequencing data confirmed our qRT-PCR results and provided evidence for selective miRNA secretion in EVs. To our knowledge, this is the first time that miRNAs have been identified in the extracellular medium of cultured cells derived from insects, the most speciose group of animals.
Keywords
SMALL RNAS, CIRCULATING MICRORNAS, BIOMARKERS, MIRNA, EXOSOMES, IDENTIFICATION, DIAGNOSIS, COMPLEX, CANCER, ROLES

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Chicago
Van den Brande, Stijn, Marijke Gijbels, Niels Wynant, Dulce Santos, Lina Mingels, Yannick Gansemans, Filip Van Nieuwerburgh, and Jozef Vanden Broeck. 2018. “The Presence of Extracellular microRNAs in the Media of Cultured Drosophila Cells.” Scientific Reports 8.
APA
Van den Brande, S., Gijbels, M., Wynant, N., Santos, D., Mingels, L., Gansemans, Y., Van Nieuwerburgh, F., et al. (2018). The presence of extracellular microRNAs in the media of cultured Drosophila cells. SCIENTIFIC REPORTS, 8.
Vancouver
1.
Van den Brande S, Gijbels M, Wynant N, Santos D, Mingels L, Gansemans Y, et al. The presence of extracellular microRNAs in the media of cultured Drosophila cells. SCIENTIFIC REPORTS. 2018;8.
MLA
Van den Brande, Stijn et al. “The Presence of Extracellular microRNAs in the Media of Cultured Drosophila Cells.” SCIENTIFIC REPORTS 8 (2018): n. pag. Print.
@article{8600689,
  abstract     = {While regulatory RNA pathways, such as RNAi, have commonly been described at an intracellular level, studies investigating extracellular RNA species in insects are lacking. In the present study, we demonstrate the presence of extracellular microRNAs (miRNAs) in the cell-free conditioned media of two Drosophila cell lines. More specifically, by means of quantitative real-time PCR (qRT-PCR), we analysed the presence of twelve miRNAs in extracellular vesicles (EVs) and in extracellular Argonaute-1 containing immunoprecipitates, obtained from the cell-free conditioned media of S2 and Cl. 8 cell cultures. Next-generation RNA-sequencing data confirmed our qRT-PCR results and provided evidence for selective miRNA secretion in EVs. To our knowledge, this is the first time that miRNAs have been identified in the extracellular medium of cultured cells derived from insects, the most speciose group of animals.},
  articleno    = {17312},
  author       = {Van den Brande, Stijn and Gijbels, Marijke and Wynant, Niels and Santos, Dulce and Mingels, Lina and Gansemans, Yannick and Van Nieuwerburgh, Filip and Vanden Broeck, Jozef},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  language     = {eng},
  pages        = {10},
  title        = {The presence of extracellular microRNAs in the media of cultured Drosophila cells},
  url          = {http://dx.doi.org/10.1038/s41598-018-35531-z},
  volume       = {8},
  year         = {2018},
}

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