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Molecular characterization of ecdysis triggering hormone and its receptor in citrus red mite (Panonychus citri)

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Abstract
Neuropeptide ecdysis triggering hormone (ETH) plays crucial roles in invertebrates by activating a G protein-coupled receptor (GPCR), the ecdysis triggering hormone receptor (ETHR), which has been intensively investigated in Hexapoda. However, the molecular characterization of ETH and ETHR in Chelicerata remains unknown. In this study, we identified and characterized the full length cDNA of ETH and ETHR in citrus red mite Panonychus citri (McGregor). Phylogenetic analysis indicated that PcETHR was closely related to the insect ETHR subtype B. A calcium mobilization-based functional assay showed that PcETH activated the PcETHR in a dose-dependent manner. Furthermore, a sharp upregulation of PcETH before ecdysis was observed by quantitative real time PCR (qRT-PCR), and it revealed a tight correlation of ETH signaling and the molting process. These are the first insights into the molecular characterization of ETH and its receptor in mites as P. cirri. Our work provides basic information of the ETH and ETHR in P. citri and lays a solid foundation for further physiological functions to better understand the ETH signaling system in mites.
Keywords
Ecdysis triggering hormone, G protein-coupled receptor, Panonychus citri, Transcriptional expression, PROTEIN COUPLED RECEPTORS, GENE-EXPRESSION, CLONING, INSECT, IDENTIFICATION, NEUROPEPTIDES, TARGETS, PCR

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MLA
Zhu, Lin, et al. “Molecular Characterization of Ecdysis Triggering Hormone and Its Receptor in Citrus Red Mite (Panonychus Citri).” COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, vol. 230, 2019, pp. 100–05.
APA
Zhu, L., Zhang, W., Li, G., Sun, Q.-Z., Wang, J.-J., Smagghe, G., & Jiang, H.-B. (2019). Molecular characterization of ecdysis triggering hormone and its receptor in citrus red mite (Panonychus citri). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 230, 100–105.
Chicago author-date
Zhu, Lin, Wei Zhang, Gang Li, Qin-Zhe Sun, Jin-Jun Wang, Guy Smagghe, and Hong-Bo Jiang. 2019. “Molecular Characterization of Ecdysis Triggering Hormone and Its Receptor in Citrus Red Mite (Panonychus Citri).” COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 230: 100–105.
Chicago author-date (all authors)
Zhu, Lin, Wei Zhang, Gang Li, Qin-Zhe Sun, Jin-Jun Wang, Guy Smagghe, and Hong-Bo Jiang. 2019. “Molecular Characterization of Ecdysis Triggering Hormone and Its Receptor in Citrus Red Mite (Panonychus Citri).” COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 230: 100–105.
Vancouver
1.
Zhu L, Zhang W, Li G, Sun Q-Z, Wang J-J, Smagghe G, et al. Molecular characterization of ecdysis triggering hormone and its receptor in citrus red mite (Panonychus citri). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY. 2019;230:100–5.
IEEE
[1]
L. Zhu et al., “Molecular characterization of ecdysis triggering hormone and its receptor in citrus red mite (Panonychus citri),” COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, vol. 230, pp. 100–105, 2019.
@article{8600583,
  abstract     = {Neuropeptide ecdysis triggering hormone (ETH) plays crucial roles in invertebrates by activating a G protein-coupled receptor (GPCR), the ecdysis triggering hormone receptor (ETHR), which has been intensively investigated in Hexapoda. However, the molecular characterization of ETH and ETHR in Chelicerata remains unknown. In this study, we identified and characterized the full length cDNA of ETH and ETHR in citrus red mite Panonychus citri (McGregor). Phylogenetic analysis indicated that PcETHR was closely related to the insect ETHR subtype B. A calcium mobilization-based functional assay showed that PcETH activated the PcETHR in a dose-dependent manner. Furthermore, a sharp upregulation of PcETH before ecdysis was observed by quantitative real time PCR (qRT-PCR), and it revealed a tight correlation of ETH signaling and the molting process. These are the first insights into the molecular characterization of ETH and its receptor in mites as P. cirri. Our work provides basic information of the ETH and ETHR in P. citri and lays a solid foundation for further physiological functions to better understand the ETH signaling system in mites.},
  author       = {Zhu, Lin and Zhang, Wei and Li, Gang and Sun, Qin-Zhe and Wang, Jin-Jun and Smagghe, Guy and Jiang, Hong-Bo},
  issn         = {1095-6433},
  journal      = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY},
  keywords     = {Ecdysis triggering hormone,G protein-coupled receptor,Panonychus citri,Transcriptional expression,PROTEIN COUPLED RECEPTORS,GENE-EXPRESSION,CLONING,INSECT,IDENTIFICATION,NEUROPEPTIDES,TARGETS,PCR},
  language     = {eng},
  pages        = {100--105},
  title        = {Molecular characterization of ecdysis triggering hormone and its receptor in citrus red mite (Panonychus citri)},
  url          = {http://dx.doi.org/10.1016/j.cbpa.2019.01.003},
  volume       = {230},
  year         = {2019},
}

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