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Biogenic silver for disinfection of water contaminated with viruses

Bart De Gusseme (UGent), Liesje Sintubin (UGent), Leen Baert (UGent), Ellen Thibo, Tom Hennebel (UGent), Griet Vermeulen (UGent), Mieke Uyttendaele (UGent), Willy Verstraete (UGent) and Nico Boon (UGent)
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Abstract
The presence of enteric viruses in drinking water is a potential health risk. Growing interest has arisen in nanometals for water disinfection, in particular the use of silver-based nanotechnology. In this study, Lactobacillus fermentum served as a reducing agent and bacterial carrier matrix for zerovalent silver nanoparticles, referred to as biogenic Ag-0. The antiviral action of biogenic Ag-0 was examined in water spiked with an Enterobacter aerogenes-infecting bacteriophage (UZ1). Addition of 5.4 mg liter(-1) biogenic Ag-0 caused a 4.0-log decrease of the phage after 1 h, whereas the use of chemically produced silver nanoparticles (nAg(0)) showed no inactivation within the same time frame. A control experiment with 5.4 mg liter(-1) ionic Ag+ resulted in a similar inactivation after 5 h only. The antiviral properties of biogenic Ag-0 were also demonstrated on the murine norovirus 1 (MNV-1), a model organism for human noroviruses. Biogenic Ag-0 was applied to an electropositive cartridge filter (NanoCeram) to evaluate its capacity for continuous disinfection. Addition of 31.25 mg biogenic Ag-0 m(-2) on the filter (135 mg biogenic Ag-0 kg(-1) filter medium) caused a 3.8-log decline of the virus. In contrast, only a 1.5-log decrease could be obtained with the original filter. This is the first report to demonstrate the antiviral efficacy of extracellular biogenic Ag-0 and its promising opportunities for continuous water disinfection.
Keywords
ESCHERICHIA-COLI, STAPHYLOCOCCUS-AUREUS, MURINE NOROVIRUS, NANOPARTICLES, NITRATE, STABILITY, BACTERIA, CELLS, MODEL, IONS

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Chicago
De Gusseme, Bart, Liesje Sintubin, Leen Baert, Ellen Thibo, Tom Hennebel, Griet Vermeulen, Mieke Uyttendaele, Willy Verstraete, and Nico Boon. 2010. “Biogenic Silver for Disinfection of Water Contaminated with Viruses.” Applied and Environmental Microbiology 76 (4): 1082–1087.
APA
De Gusseme, B., Sintubin, L., Baert, L., Thibo, E., Hennebel, T., Vermeulen, G., Uyttendaele, M., et al. (2010). Biogenic silver for disinfection of water contaminated with viruses. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(4), 1082–1087.
Vancouver
1.
De Gusseme B, Sintubin L, Baert L, Thibo E, Hennebel T, Vermeulen G, et al. Biogenic silver for disinfection of water contaminated with viruses. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2010;76(4):1082–7.
MLA
De Gusseme, Bart, Liesje Sintubin, Leen Baert, et al. “Biogenic Silver for Disinfection of Water Contaminated with Viruses.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 76.4 (2010): 1082–1087. Print.
@article{860053,
  abstract     = {The presence of enteric viruses in drinking water is a potential health risk. Growing interest has arisen in nanometals for water disinfection, in particular the use of silver-based nanotechnology. In this study, Lactobacillus fermentum served as a reducing agent and bacterial carrier matrix for zerovalent silver nanoparticles, referred to as biogenic Ag-0. The antiviral action of biogenic Ag-0 was examined in water spiked with an Enterobacter aerogenes-infecting bacteriophage (UZ1). Addition of 5.4 mg liter(-1) biogenic Ag-0 caused a 4.0-log decrease of the phage after 1 h, whereas the use of chemically produced silver nanoparticles (nAg(0)) showed no inactivation within the same time frame. A control experiment with 5.4 mg liter(-1) ionic Ag+ resulted in a similar inactivation after 5 h only. The antiviral properties of biogenic Ag-0 were also demonstrated on the murine norovirus 1 (MNV-1), a model organism for human noroviruses. Biogenic Ag-0 was applied to an electropositive cartridge filter (NanoCeram) to evaluate its capacity for continuous disinfection. Addition of 31.25 mg biogenic Ag-0 m(-2) on the filter (135 mg biogenic Ag-0 kg(-1) filter medium) caused a 3.8-log decline of the virus. In contrast, only a 1.5-log decrease could be obtained with the original filter. This is the first report to demonstrate the antiviral efficacy of extracellular biogenic Ag-0 and its promising opportunities for continuous water disinfection.},
  author       = {De Gusseme, Bart and Sintubin, Liesje and Baert, Leen and Thibo, Ellen and Hennebel, Tom and Vermeulen, Griet and Uyttendaele, Mieke and Verstraete, Willy and Boon, Nico},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {ESCHERICHIA-COLI,STAPHYLOCOCCUS-AUREUS,MURINE NOROVIRUS,NANOPARTICLES,NITRATE,STABILITY,BACTERIA,CELLS,MODEL,IONS},
  language     = {eng},
  number       = {4},
  pages        = {1082--1087},
  title        = {Biogenic silver for disinfection of water contaminated with viruses},
  url          = {http://dx.doi.org/10.1128/AEM.02433-09},
  volume       = {76},
  year         = {2010},
}

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