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DOF2.1 controls cytokinin-dependent vascular cell proliferation downstream of TMO5/LHW

Wouter Smet (UGent) , Iris Sevilem, Maria Angels de Luis Balaguer, Brecht Wybouw, Eliana Mor, Shunsuke Miyashima, Bernhard Blob, Pawel Roszak, Thomas B. Jacobs (UGent) , Mark Boekschoten, et al.
(2019) CURRENT BIOLOGY. 29(3). p.520-529
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Abstract
To create a three-dimensional structure, plants rely on oriented cell divisions and cell elongation. Oriented cell divisions are specifically important in procambium cells of the root to establish the different vascular cell types [1, 2]. These divisions are in part controlled by the auxin-controlled TARGET OF MONOPTEROS5 (TMO5) and LONESOME HIGHWAY (LHW) transcription factor complex [3-7]. Loss-of-function of tmo5 or lhw clade members results in strongly reduced vascular cell file numbers, whereas ectopic expression of both TMO5 and LHW can ubiquitously induce periclinal and radial cell divisions in all cell types of the root meristem. TMO5 and LHW interact only in young xylem cells, where they promote expression of two direct target genes involved in the final step of cytokinin (CK) biosynthesis, LONELY GUY3 (LOG3) and LOG4 [8, 9] Therefore, CK was hypothesized to act as a mobile signal from the xylem to trigger divisions in the neighboring procambium cells [3, 6]. To unravel how TMO5/LHW-dependent cytokinin regulates cell proliferation, we analyzed the transcriptional responses upon simultaneous induction of both transcription factors. Using inferred network analysis, we identified AT2G28510/DOF2.1 as a cytokinin-dependent downstream target gene. We further showed that DOF2.1 controls specific procambium cell divisions without inducing other cytokinin-dependent effects such as the inhibition of vascular differentiation. In summary, our results suggest that DOF2.1 and its closest homologs control vascular cell proliferation, thus leading to radial expansion of the root.
Keywords
LIGATION-INDEPENDENT CLONING, RESPONSE REGULATOR, ARABIDOPSIS, GENE, EXPRESSION, DIFFERENTIATION, ORGANIZATION, INITIATION, DIVISION, PATHWAY

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MLA
Smet, Wouter, et al. “DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW.” CURRENT BIOLOGY, vol. 29, no. 3, 2019, pp. 520–29, doi:10.1016/j.cub.2018.12.041.
APA
Smet, W., Sevilem, I., de Luis Balaguer, M. A., Wybouw, B., Mor, E., Miyashima, S., … De Rybel, B. (2019). DOF2.1 controls cytokinin-dependent vascular cell proliferation downstream of TMO5/LHW. CURRENT BIOLOGY, 29(3), 520–529. https://doi.org/10.1016/j.cub.2018.12.041
Chicago author-date
Smet, Wouter, Iris Sevilem, Maria Angels de Luis Balaguer, Brecht Wybouw, Eliana Mor, Shunsuke Miyashima, Bernhard Blob, et al. 2019. “DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW.” CURRENT BIOLOGY 29 (3): 520–29. https://doi.org/10.1016/j.cub.2018.12.041.
Chicago author-date (all authors)
Smet, Wouter, Iris Sevilem, Maria Angels de Luis Balaguer, Brecht Wybouw, Eliana Mor, Shunsuke Miyashima, Bernhard Blob, Pawel Roszak, Thomas B. Jacobs, Mark Boekschoten, Guido Hooiveld, Rosangela Sozzani, Ykä Helariutta, and Bert De Rybel. 2019. “DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW.” CURRENT BIOLOGY 29 (3): 520–529. doi:10.1016/j.cub.2018.12.041.
Vancouver
1.
Smet W, Sevilem I, de Luis Balaguer MA, Wybouw B, Mor E, Miyashima S, et al. DOF2.1 controls cytokinin-dependent vascular cell proliferation downstream of TMO5/LHW. CURRENT BIOLOGY. 2019;29(3):520–9.
IEEE
[1]
W. Smet et al., “DOF2.1 controls cytokinin-dependent vascular cell proliferation downstream of TMO5/LHW,” CURRENT BIOLOGY, vol. 29, no. 3, pp. 520–529, 2019.
@article{8600510,
  abstract     = {{To create a three-dimensional structure, plants rely on oriented cell divisions and cell elongation. Oriented cell divisions are specifically important in procambium cells of the root to establish the different vascular cell types [1, 2]. These divisions are in part controlled by the auxin-controlled TARGET OF MONOPTEROS5 (TMO5) and LONESOME HIGHWAY (LHW) transcription factor complex [3-7]. Loss-of-function of tmo5 or lhw clade members results in strongly reduced vascular cell file numbers, whereas ectopic expression of both TMO5 and LHW can ubiquitously induce periclinal and radial cell divisions in all cell types of the root meristem. TMO5 and LHW interact only in young xylem cells, where they promote expression of two direct target genes involved in the final step of cytokinin (CK) biosynthesis, LONELY GUY3 (LOG3) and LOG4 [8, 9] Therefore, CK was hypothesized to act as a mobile signal from the xylem to trigger divisions in the neighboring procambium cells [3, 6]. To unravel how TMO5/LHW-dependent cytokinin regulates cell proliferation, we analyzed the transcriptional responses upon simultaneous induction of both transcription factors. Using inferred network analysis, we identified AT2G28510/DOF2.1 as a cytokinin-dependent downstream target gene. We further showed that DOF2.1 controls specific procambium cell divisions without inducing other cytokinin-dependent effects such as the inhibition of vascular differentiation. In summary, our results suggest that DOF2.1 and its closest homologs control vascular cell proliferation, thus leading to radial expansion of the root.}},
  author       = {{Smet, Wouter and Sevilem, Iris and de Luis Balaguer, Maria Angels and Wybouw, Brecht and Mor, Eliana and Miyashima, Shunsuke and Blob, Bernhard and Roszak, Pawel and Jacobs, Thomas B. and Boekschoten, Mark and Hooiveld, Guido and Sozzani, Rosangela and Helariutta, Ykä and De Rybel, Bert}},
  issn         = {{0960-9822}},
  journal      = {{CURRENT BIOLOGY}},
  keywords     = {{LIGATION-INDEPENDENT CLONING,RESPONSE REGULATOR,ARABIDOPSIS,GENE,EXPRESSION,DIFFERENTIATION,ORGANIZATION,INITIATION,DIVISION,PATHWAY}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{520--529}},
  title        = {{DOF2.1 controls cytokinin-dependent vascular cell proliferation downstream of TMO5/LHW}},
  url          = {{http://doi.org/10.1016/j.cub.2018.12.041}},
  volume       = {{29}},
  year         = {{2019}},
}
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