Advanced search
1 file | 1.78 MB

Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing

(2019) Nature Plants. 5(2). p.225-237
Author
Organization
Abstract
Lignin is the main cause of lignocellulosic biomass recalcitrance to industrial enzymatic hydrolysis. By partially replacing the traditional lignin monomers by alternative ones, lignin extractability can be enhanced. To design a lignin that is easier to degrade under alkaline conditions, curcumin (diferuloylmethane) was produced in the model plant Arabidopsis thaliana via simultaneous expression of the turmeric (Curcuma longa) genes DIKETIDE-CoA SYNTHASE (DCS) and CURCUMIN SYNTHASE 2 (CURS2). The transgenic plants produced a plethora of curcumin- and phenylpentanoid-derived compounds with no negative impact on growth. Catalytic hydrogenolysis gave evidence that both curcumin and phenylpentanoids were incorporated into the lignifying cell wall, thereby significantly increasing saccharification efficiency after alkaline pretreatment of the transgenic lines by 14–24% as compared with the wild type. These results demonstrate that non-native monomers can be synthesized and incorporated into the lignin polymer in plants to enhance their biomass processing efficiency.

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.78 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Oyarce Sgro, Paula, Barbara De Meester, Fernando Campos De Assis Fonseca, Lisanne de Vries, Geert Goeminne, Andreas Pallidis, Riet De Rycke, et al. 2019. “Introducing Curcumin Biosynthesis in Arabidopsis Enhances Lignocellulosic Biomass Processing.” Nature Plants 5 (2): 225–237.
APA
Oyarce Sgro, P., De Meester, B., Campos De Assis Fonseca, F., de Vries, L., Goeminne, G., Pallidis, A., De Rycke, R., et al. (2019). Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing. Nature Plants, 5(2), 225–237.
Vancouver
1.
Oyarce Sgro P, De Meester B, Campos De Assis Fonseca F, de Vries L, Goeminne G, Pallidis A, et al. Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing. Nature Plants. Springer Nature; 2019;5(2):225–37.
MLA
Oyarce Sgro, Paula et al. “Introducing Curcumin Biosynthesis in Arabidopsis Enhances Lignocellulosic Biomass Processing.” Nature Plants 5.2 (2019): 225–237. Print.
@article{8600508,
  abstract     = {Lignin is the main cause of lignocellulosic biomass recalcitrance to industrial enzymatic hydrolysis. By partially replacing the traditional lignin monomers by alternative ones, lignin extractability can be enhanced. To design a lignin that is easier to degrade under alkaline conditions, curcumin (diferuloylmethane) was produced in the model plant Arabidopsis thaliana via simultaneous expression of the turmeric (Curcuma longa) genes DIKETIDE-CoA SYNTHASE (DCS) and CURCUMIN SYNTHASE 2 (CURS2). The transgenic plants produced a plethora of curcumin- and phenylpentanoid-derived compounds with no negative impact on growth. Catalytic hydrogenolysis gave evidence that both curcumin and phenylpentanoids were incorporated into the lignifying cell wall, thereby significantly increasing saccharification efficiency after alkaline pretreatment of the transgenic lines by 14--24\% as compared with the wild type. These results demonstrate that non-native monomers can be synthesized and incorporated into the lignin polymer in plants to enhance their biomass processing efficiency.},
  author       = {Oyarce Sgro, Paula and De Meester, Barbara and Campos De Assis Fonseca, Fernando and de Vries, Lisanne and Goeminne, Geert and Pallidis, Andreas and De Rycke, Riet and Tsuji, Yukiko and Li, Yanding and Van den Bosch, Sander and Sels, Bert and Ralph, John and Vanholme, Ruben and Boerjan, Wout},
  issn         = {2055-0278},
  journal      = {Nature Plants},
  language     = {eng},
  number       = {2},
  pages        = {225--237},
  publisher    = {Springer Nature},
  title        = {Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing},
  url          = {http://dx.doi.org/10.1038/s41477-018-0350-3},
  volume       = {5},
  year         = {2019},
}

Altmetric
View in Altmetric