Advanced search
1 file | 398.38 KB Add to list

Gravity-related immunological changes in human whole blood cultured under simulated microgravity using an in vitro cytokine release assay

Author
Organization
Abstract
Although immune dysfunction by space conditions has been reported postflight, as well as during ground-based experiments, the cause(s) and nature of the immunological changes are not completely understood. Microgravity has been suggested as one of the factors responsible for the observed immune dysregulation. The goal of this study was to assess immune changes in simulated microgravity (s-G) using an in vitro cytokine release assay. The effect of s-G provided by the desktop random positioning machine on cell-mediated immunity was examined by analyzing interleukin 2 (IL-2), interferon- (IFN-), tumor necrosis factor alpha (TNF-), and interleukin 10 (IL-10), in response to immune cell stimulation in whole blood samples (n=10). Stimuli used were bacterial recall antigens, pokeweed mitogen (PWM), lipopolysaccharide (LPS), or heat-killed Listeria monocytogenes (HKLM). S-G caused an overall inhibition of the IL-2 and IFN- responses to recall antigen and mitogen stimulation. More specifically, s-G most strongly influenced the levels of all four cytokines elicited by bacterial recall antigen stimulation. In contrast, HKLM-induced TNF- secretion was elevated. The average concentrations of TNF- in response to PWM and LPS and IL-10 release stimulated by PWM, LPS, and HKLM were not significantly altered by s-G. However, a variable response between individual subjects could be observed. In conclusion, our results demonstrate that the in vitro cytokine release assay can detect gravity-related immune alterations. Furthermore, the use of multiple stimuli and the associated changes in cytokine secretion has the potential to reveal information on the underlying mechanisms affected by s-G.
Keywords
cell-mediated immune response, simulated microgravity, random positioning machine, whole blood, in vitro cytokine release assay, LONG-DURATION SPACEFLIGHT, IMMUNE-SYSTEM DYSREGULATION, T-LYMPHOCYTES, SPACE-FLIGHT, SIGNAL-TRANSDUCTION, ACTIVATION, INFLAMMATION, SUPERFAMILY, EXPLORATION, RECEPTOR

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 398.38 KB

Citation

Please use this url to cite or link to this publication:

MLA
Van Walleghem, Merel et al. “Gravity-related Immunological Changes in Human Whole Blood Cultured Under Simulated Microgravity Using an in Vitro Cytokine Release Assay.” JOURNAL OF INTERFERON AND CYTOKINE RESEARCH 37.12 (2017): 531–540. Print.
APA
Van Walleghem, M., Tabury, K., Fernandez Gonzalo, R., Janssen, A., Buchheim, J.-I., Choukèr, A., Baatout, S., et al. (2017). Gravity-related immunological changes in human whole blood cultured under simulated microgravity using an in vitro cytokine release assay. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 37(12), 531–540.
Chicago author-date
Van Walleghem, Merel, Kevin Tabury, Rodrigo Fernandez Gonzalo, Ann Janssen, Judith-Irina Buchheim, Alexander Choukèr, Sarah Baatout, and Marjan Moreels. 2017. “Gravity-related Immunological Changes in Human Whole Blood Cultured Under Simulated Microgravity Using an in Vitro Cytokine Release Assay.” Journal of Interferon and Cytokine Research 37 (12): 531–540.
Chicago author-date (all authors)
Van Walleghem, Merel, Kevin Tabury, Rodrigo Fernandez Gonzalo, Ann Janssen, Judith-Irina Buchheim, Alexander Choukèr, Sarah Baatout, and Marjan Moreels. 2017. “Gravity-related Immunological Changes in Human Whole Blood Cultured Under Simulated Microgravity Using an in Vitro Cytokine Release Assay.” Journal of Interferon and Cytokine Research 37 (12): 531–540.
Vancouver
1.
Van Walleghem M, Tabury K, Fernandez Gonzalo R, Janssen A, Buchheim J-I, Choukèr A, et al. Gravity-related immunological changes in human whole blood cultured under simulated microgravity using an in vitro cytokine release assay. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH. 2017;37(12):531–40.
IEEE
[1]
M. Van Walleghem et al., “Gravity-related immunological changes in human whole blood cultured under simulated microgravity using an in vitro cytokine release assay,” JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, vol. 37, no. 12, pp. 531–540, 2017.
@article{8600344,
  abstract     = {Although immune dysfunction by space conditions has been reported postflight, as well as during ground-based experiments, the cause(s) and nature of the immunological changes are not completely understood. Microgravity has been suggested as one of the factors responsible for the observed immune dysregulation. The goal of this study was to assess immune changes in simulated microgravity (s-G) using an in vitro cytokine release assay. The effect of s-G provided by the desktop random positioning machine on cell-mediated immunity was examined by analyzing interleukin 2 (IL-2), interferon- (IFN-), tumor necrosis factor alpha (TNF-), and interleukin 10 (IL-10), in response to immune cell stimulation in whole blood samples (n=10). Stimuli used were bacterial recall antigens, pokeweed mitogen (PWM), lipopolysaccharide (LPS), or heat-killed Listeria monocytogenes (HKLM). S-G caused an overall inhibition of the IL-2 and IFN- responses to recall antigen and mitogen stimulation. More specifically, s-G most strongly influenced the levels of all four cytokines elicited by bacterial recall antigen stimulation. In contrast, HKLM-induced TNF- secretion was elevated. The average concentrations of TNF- in response to PWM and LPS and IL-10 release stimulated by PWM, LPS, and HKLM were not significantly altered by s-G. However, a variable response between individual subjects could be observed. In conclusion, our results demonstrate that the in vitro cytokine release assay can detect gravity-related immune alterations. Furthermore, the use of multiple stimuli and the associated changes in cytokine secretion has the potential to reveal information on the underlying mechanisms affected by s-G.},
  author       = {Van Walleghem, Merel and Tabury, Kevin and Fernandez Gonzalo, Rodrigo and Janssen, Ann and Buchheim, Judith-Irina and Choukèr, Alexander and Baatout, Sarah and Moreels, Marjan},
  issn         = {1079-9907},
  journal      = {JOURNAL OF INTERFERON AND CYTOKINE RESEARCH},
  keywords     = {cell-mediated immune response,simulated microgravity,random positioning machine,whole blood,in vitro cytokine release assay,LONG-DURATION SPACEFLIGHT,IMMUNE-SYSTEM DYSREGULATION,T-LYMPHOCYTES,SPACE-FLIGHT,SIGNAL-TRANSDUCTION,ACTIVATION,INFLAMMATION,SUPERFAMILY,EXPLORATION,RECEPTOR},
  language     = {eng},
  number       = {12},
  pages        = {531--540},
  title        = {Gravity-related immunological changes in human whole blood cultured under simulated microgravity using an in vitro cytokine release assay},
  url          = {http://dx.doi.org/10.1089/jir.2017.0065},
  volume       = {37},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: