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RNA-directed proteomics to explore the interactome of the melanoma-specific lncRNA SAMMSON

Louis Delhaye (UGent) , Shanna Dewaele (UGent) , Delphine De Sutter (UGent) , Pieter Mestdagh (UGent) and Sven Eyckerman (UGent)
(2019)
Author
Organization
Abstract
The last decade, there has been an increasing support for the involvement of long non-coding RNAs (lncRNAs) in both tumor promoting and suppressing mechanisms in human cancers. Although functional proof for the bulk of these lncRNAs is still lacking, most lncRNAs act as protein guides, decoys or as modular scaffolds for the formation of protein complexes. Studying these lncRNA-protein interaction partners provides crucial information on the functional mechanisms of these cancerassociated lncRNAs. Recently, we identified the lineage-specific oncogenic lncRNA SAMMSON that is essential for melanoma cell survival. Here, we combine the RNA-directed proteomic technique ChIRPMS with label-free quantification (LFQ) to further explore the SAMMSON interactome in different melanoma cell lines. Our LFQ data confirms the interaction with p32/C1QBP and XRN2. In addition, we identify multiple mitochondrial ribosomal and nucleolar rRNA-processing proteins confirming SAMMSON’s role in the modulation of mitochondrial and cellular protein synthesis. Our data supports the role of SAMMSON and its interaction with p32/C1QBP and XRN2 in modulation of mitochondrial and cellular protein synthesis balances for pro-oncogenic rewiring of melanoma.
Keywords
lncRNA, SAMMSON, ChIRP-MS, melanoma

Citation

Please use this url to cite or link to this publication:

Chicago
Delhaye, Louis, Shanna Dewaele, Delphine De Sutter, Pieter Mestdagh, and Sven Eyckerman. 2019. “RNA-directed Proteomics to Explore the Interactome of the Melanoma-specific lncRNA SAMMSON.” In .
APA
Delhaye, L., Dewaele, S., De Sutter, D., Mestdagh, P., & Eyckerman, S. (2019). RNA-directed proteomics to explore the interactome of the melanoma-specific lncRNA SAMMSON. Presented at the Long noncoding RNAs: from molecular mechanism to functional genetics.
Vancouver
1.
Delhaye L, Dewaele S, De Sutter D, Mestdagh P, Eyckerman S. RNA-directed proteomics to explore the interactome of the melanoma-specific lncRNA SAMMSON. 2019.
MLA
Delhaye, Louis et al. “RNA-directed Proteomics to Explore the Interactome of the Melanoma-specific lncRNA SAMMSON.” 2019. Print.
@inproceedings{8599539,
  abstract     = {The last decade, there has been an increasing support for the involvement of long non-coding RNAs
(lncRNAs) in both tumor promoting and suppressing mechanisms in human cancers. Although
functional proof for the bulk of these lncRNAs is still lacking, most lncRNAs act as protein guides,
decoys or as modular scaffolds for the formation of protein complexes. Studying these lncRNA-protein
interaction partners provides crucial information on the functional mechanisms of these cancerassociated
lncRNAs. Recently, we identified the lineage-specific oncogenic lncRNA SAMMSON that is
essential for melanoma cell survival. Here, we combine the RNA-directed proteomic technique ChIRPMS
with label-free quantification (LFQ) to further explore the SAMMSON interactome in different
melanoma cell lines. Our LFQ data confirms the interaction with p32/C1QBP and XRN2. In addition,
we identify multiple mitochondrial ribosomal and nucleolar rRNA-processing proteins confirming
SAMMSON{\textquoteright}s role in the modulation of mitochondrial and cellular protein synthesis. Our data supports
the role of SAMMSON and its interaction with p32/C1QBP and XRN2 in modulation of mitochondrial
and cellular protein synthesis balances for pro-oncogenic rewiring of melanoma.},
  author       = {Delhaye, Louis and Dewaele, Shanna and De Sutter, Delphine and Mestdagh, Pieter and Eyckerman, Sven},
  location     = {Canada, Whistler},
  title        = {RNA-directed proteomics to explore the interactome of the melanoma-specific lncRNA SAMMSON},
  year         = {2019},
}