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Allogeneic mesenchymal stem cells improve the wound healing process of sheep skin

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Abstract
Background: Skin wound healing includes a system of biological processes, collectively restoring the integrity of the skin after injury. Healing by second intention refers to repair of large and deep wounds where the tissue edges cannot be approximated and substantial scarring is often observed. The objective of this study was to evaluate the effects of mesenchymal stem cells (MSCs) in second intention healing using a surgical wound model in sheep. MSCs are known to contribute to the inflammatory, proliferative, and remodeling phases of the skin regeneration process in rodent models, but data are lacking for large animal models. This study used three different approaches (clinical, histopathological, and molecular analysis) to assess the putative action of allogeneic MSCs at 15 and 42 days after lesion creation. Results: At 15 days post-lesion, the wounds treated with MSCs showed a higher degree of wound closure, a higher percentage of re-epithelialization, proliferation, neovascularization and increased contraction in comparison to a control group. At 42 days, the wounds treated with MSCs had more mature and denser cutaneous adnexa compared to the control group. The MSCs-treated group showed an absence of inflammation and expression of CD3+ and CD20+. Moreover, the mRNA expression of hair-keratine (hKER) was observed in the MSCs-treated group 15 days after wound creation and had increased significantly by 42 days post-wound creation. Collagen1 gene (Col1a1) expression was also greater in the MSCs-treated group compared to the control group at both days 15 and 42. Conclusion: Peripheral blood-derived MSCs may improve the quality of wound healing both for superficial injuries and deep lesions. MSCs did not induce an inflammatory response and accelerated the appearance of granulation tissue, neovascularization, structural proteins, and skin adnexa.
Keywords
NUCLEATED CELLS, GROWTH-FACTORS, THERAPY, REPAIR, CRYOPRESERVATION, TRANSPLANTATION, REGENERATION, BLOOD, MODEL, RATS, Wound healing, Mesenchymal stem cells, Cell therapy, Regenerative, medicine

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Chicago
Martinello, T, C Gomiero, A Perazzi, I Lacopetti, F Gemignani, GM DeBenedictis, S Ferro, et al. 2018. “Allogeneic Mesenchymal Stem Cells Improve the Wound Healing Process of Sheep Skin.” Bmc Veterinary Research 14.
APA
Martinello, T., Gomiero, C., Perazzi, A., Lacopetti, I., Gemignani, F., DeBenedictis, G., Ferro, S., et al. (2018). Allogeneic mesenchymal stem cells improve the wound healing process of sheep skin. BMC VETERINARY RESEARCH, 14.
Vancouver
1.
Martinello T, Gomiero C, Perazzi A, Lacopetti I, Gemignani F, DeBenedictis G, et al. Allogeneic mesenchymal stem cells improve the wound healing process of sheep skin. BMC VETERINARY RESEARCH. 2018;14.
MLA
Martinello, T et al. “Allogeneic Mesenchymal Stem Cells Improve the Wound Healing Process of Sheep Skin.” BMC VETERINARY RESEARCH 14 (2018): n. pag. Print.
@article{8599456,
  abstract     = {Background: Skin wound healing includes a system of biological processes, collectively restoring the integrity of the skin after injury. Healing by second intention refers to repair of large and deep wounds where the tissue edges cannot be approximated and substantial scarring is often observed. The objective of this study was to evaluate the effects of mesenchymal stem cells (MSCs) in second intention healing using a surgical wound model in sheep. MSCs are known to contribute to the inflammatory, proliferative, and remodeling phases of the skin regeneration process in rodent models, but data are lacking for large animal models. This study used three different approaches (clinical, histopathological, and molecular analysis) to assess the putative action of allogeneic MSCs at 15 and 42 days after lesion creation. 
Results: At 15 days post-lesion, the wounds treated with MSCs showed a higher degree of wound closure, a higher percentage of re-epithelialization, proliferation, neovascularization and increased contraction in comparison to a control group. At 42 days, the wounds treated with MSCs had more mature and denser cutaneous adnexa compared to the control group. The MSCs-treated group showed an absence of inflammation and expression of CD3+ and CD20+. Moreover, the mRNA expression of hair-keratine (hKER) was observed in the MSCs-treated group 15 days after wound creation and had increased significantly by 42 days post-wound creation. Collagen1 gene (Col1a1) expression was also greater in the MSCs-treated group compared to the control group at both days 15 and 42. 
Conclusion: Peripheral blood-derived MSCs may improve the quality of wound healing both for superficial injuries and deep lesions. MSCs did not induce an inflammatory response and accelerated the appearance of granulation tissue, neovascularization, structural proteins, and skin adnexa.},
  articleno    = {202},
  author       = {Martinello, T and Gomiero, C and Perazzi, A and Lacopetti, I and Gemignani, F and DeBenedictis, GM and Ferro, S and Zuin, M and Martines, E and Brun, P and Maccatrozzo, L and Chiers, Koen and Spaas, JH and Patruno, M},
  issn         = {1746-6148},
  journal      = {BMC VETERINARY RESEARCH},
  keywords     = {NUCLEATED CELLS,GROWTH-FACTORS,THERAPY,REPAIR,CRYOPRESERVATION,TRANSPLANTATION,REGENERATION,BLOOD,MODEL,RATS,Wound healing,Mesenchymal stem cells,Cell therapy,Regenerative,medicine},
  language     = {eng},
  pages        = {9},
  title        = {Allogeneic mesenchymal stem cells improve the wound healing process of sheep skin},
  url          = {http://dx.doi.org/10.1186/s12917-018-1527-8},
  volume       = {14},
  year         = {2018},
}

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