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Characterization of the γ-secretase subunit interactome in Arabidopsis thaliana

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Abstract
Gamma secretase is a multi-subunit complex with aspartic intramembrane protease activity that is involved in the pathogenesis of Alzheimer's disease in humans. In Arabidopsis thaliana, -secretase subunits are localized to endomembrane system compartments and interact with each other in a similar manner to their human counterparts. Here, we identified the protein partners of two plant -secretase subunits, presenilin 2 and PEN-2, by tandem affinity purification and co-immunoprecipitation, respectively. Integral membrane proteins were found to interact with presenilin 2, whereas secreted proteins were found to interact with PEN-2. Microscopy screening revealed that the reticulon family protein, RTNLB1, and two single transmembrane domain proteins, TIR-X and the phytolongin PHYL1.1, interact with presenilins. Finally, we show that RTNLB1 interacts with TIR-X. These results represent a step toward elucidating the functions of -secretase subunits in plant cells.
Keywords
REGULATED INTRAMEMBRANE PROTEOLYSIS, TANDEM AFFINITY PURIFICATION, AMYLOID PRECURSOR PROTEIN, GAMMA-SECRETASE, DISEASE RESISTANCE, TIR-X, SELF-ASSOCIATION, CELL-DEATH, PRESENILIN, IDENTIFICATION, Gamma-secretase, Arabidopsis proteins, Presenilins, Protein-protein, interactions, Endomembrane proteins

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  • Skrzypczak et al. (2019) Acta Physiologiae Plantarum 41,19.pdf
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Chicago
Skrzypczak, Tomasz, Rafal Krela, Shraddha Wadurkar, Kris Gevaert, Eveline Van De Slijke, Geert De Jaeger, Krzysztof Lesniewicz, and Przemyslaw Wojtaszek. 2019. “Characterization of the Γ-secretase Subunit Interactome in Arabidopsis Thaliana.” Acta Physiologiae Plantarum 41 (2).
APA
Skrzypczak, T., Krela, R., Wadurkar, S., Gevaert, K., Van De Slijke, E., De Jaeger, G., Lesniewicz, K., et al. (2019). Characterization of the γ-secretase subunit interactome in Arabidopsis thaliana. ACTA PHYSIOLOGIAE PLANTARUM, 41(2).
Vancouver
1.
Skrzypczak T, Krela R, Wadurkar S, Gevaert K, Van De Slijke E, De Jaeger G, et al. Characterization of the γ-secretase subunit interactome in Arabidopsis thaliana. ACTA PHYSIOLOGIAE PLANTARUM. 2019;41(2).
MLA
Skrzypczak, Tomasz et al. “Characterization of the Γ-secretase Subunit Interactome in Arabidopsis Thaliana.” ACTA PHYSIOLOGIAE PLANTARUM 41.2 (2019): n. pag. Print.
@article{8599236,
  abstract     = {Gamma secretase is a multi-subunit complex with aspartic intramembrane protease activity that is involved in the pathogenesis of Alzheimer's disease in humans. In Arabidopsis thaliana, -secretase subunits are localized to endomembrane system compartments and interact with each other in a similar manner to their human counterparts. Here, we identified the protein partners of two plant -secretase subunits, presenilin 2 and PEN-2, by tandem affinity purification and co-immunoprecipitation, respectively. Integral membrane proteins were found to interact with presenilin 2, whereas secreted proteins were found to interact with PEN-2. Microscopy screening revealed that the reticulon family protein, RTNLB1, and two single transmembrane domain proteins, TIR-X and the phytolongin PHYL1.1, interact with presenilins. Finally, we show that RTNLB1 interacts with TIR-X. These results represent a step toward elucidating the functions of -secretase subunits in plant cells.},
  articleno    = {19},
  author       = {Skrzypczak, Tomasz and Krela, Rafal and Wadurkar, Shraddha and Gevaert, Kris and Van De Slijke, Eveline and De Jaeger, Geert and Lesniewicz, Krzysztof and Wojtaszek, Przemyslaw},
  issn         = {0137-5881},
  journal      = {ACTA PHYSIOLOGIAE PLANTARUM},
  language     = {eng},
  number       = {2},
  pages        = {18},
  title        = {Characterization of the \ensuremath{\gamma}-secretase subunit interactome in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1007/s11738-019-2811-3},
  volume       = {41},
  year         = {2019},
}

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