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The role of HEXOKINASE1 in Arabidopsis leaf growth

Judith Van Dingenen (UGent) , Mattias Vermeersch (UGent) , Liesbeth De Milde (UGent) , Sander Hulsmans, Nancy De Winne (UGent) , Jelle Van Leene (UGent) , Nathalie Gonzalez Sanchez (UGent) , Stijn Dhondt (UGent) , Geert De Jaeger (UGent) , Filip Rolland, et al.
(2019) PLANT MOLECULAR BIOLOGY. 99(1-2). p.79-93
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Abstract
Here, we used a hxk1 mutant in the Col-0 background. We demonstrated that HXK1 regulates cell proliferation and expansion early during leaf development, and that HXK1 is involved in sucrose-induced leaf growth stimulation independent of GPT2. Furthermore, we identified KIN gamma as a novel HXK1-interacting protein. In the last decade, extensive efforts have been made to unravel the underlying mechanisms of plant growth control through sugar availability. Signaling by the conserved glucose sensor HEXOKINASE1 (HXK1) has been shown to exert both growth-promoting and growth-inhibitory effects depending on the sugar levels, the environmental conditions and the plant species. Here, we used a hxk1 mutant in the Col-0 background to investigate the role of HXK1 during leaf growth in more detail and show that it is affected in both cell proliferation and cell expansion early during leaf development. Furthermore, the hxk1 mutant is less sensitive to sucrose-induced cell proliferation with no significant increase in final leaf growth after transfer to sucrose. Early during leaf development, transfer to sucrose stimulates expression of GLUCOSE-6-PHOSPHATE/PHOSPHATE TRANSPORTER2 (GPT2) and represses chloroplast differentiation. However, in the hxk1 mutant GPT2 expression was still upregulated by transfer to sucrose although chloroplast differentiation was not affected, suggesting that GPT2 is not involved in HXK1-dependent regulation of leaf growth. Finally, using tandem affinity purification of protein complexes from cell cultures, we identified KIN gamma, a protein containing four cystathionine beta-synthase domains, as an interacting protein of HXK1.
Keywords
HEXOKINASE1, Leaf growth, Sucrose, Sink-source, TANDEM AFFINITY PURIFICATION, KINASE COMPLEX, ALTERNATIVE OXIDASE, PLANT-GROWTH, SUGAR, EXPRESSION, THALIANA, GLUCOSE, SENSOR, PROTEINS

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MLA
Van Dingenen, Judith, et al. “The Role of HEXOKINASE1 in Arabidopsis Leaf Growth.” PLANT MOLECULAR BIOLOGY, vol. 99, no. 1–2, 2019, pp. 79–93, doi:10.1007/s11103-018-0803-0.
APA
Van Dingenen, J., Vermeersch, M., De Milde, L., Hulsmans, S., De Winne, N., Van Leene, J., … Inzé, D. (2019). The role of HEXOKINASE1 in Arabidopsis leaf growth. PLANT MOLECULAR BIOLOGY, 99(1–2), 79–93. https://doi.org/10.1007/s11103-018-0803-0
Chicago author-date
Van Dingenen, Judith, Mattias Vermeersch, Liesbeth De Milde, Sander Hulsmans, Nancy De Winne, Jelle Van Leene, Nathalie Gonzalez Sanchez, et al. 2019. “The Role of HEXOKINASE1 in Arabidopsis Leaf Growth.” PLANT MOLECULAR BIOLOGY 99 (1–2): 79–93. https://doi.org/10.1007/s11103-018-0803-0.
Chicago author-date (all authors)
Van Dingenen, Judith, Mattias Vermeersch, Liesbeth De Milde, Sander Hulsmans, Nancy De Winne, Jelle Van Leene, Nathalie Gonzalez Sanchez, Stijn Dhondt, Geert De Jaeger, Filip Rolland, and Dirk Inzé. 2019. “The Role of HEXOKINASE1 in Arabidopsis Leaf Growth.” PLANT MOLECULAR BIOLOGY 99 (1–2): 79–93. doi:10.1007/s11103-018-0803-0.
Vancouver
1.
Van Dingenen J, Vermeersch M, De Milde L, Hulsmans S, De Winne N, Van Leene J, et al. The role of HEXOKINASE1 in Arabidopsis leaf growth. PLANT MOLECULAR BIOLOGY. 2019;99(1–2):79–93.
IEEE
[1]
J. Van Dingenen et al., “The role of HEXOKINASE1 in Arabidopsis leaf growth,” PLANT MOLECULAR BIOLOGY, vol. 99, no. 1–2, pp. 79–93, 2019.
@article{8599215,
  abstract     = {{Here, we used a hxk1 mutant in the Col-0 background. We demonstrated that HXK1 regulates cell proliferation and expansion early during leaf development, and that HXK1 is involved in sucrose-induced leaf growth stimulation independent of GPT2. Furthermore, we identified KIN gamma as a novel HXK1-interacting protein. 
In the last decade, extensive efforts have been made to unravel the underlying mechanisms of plant growth control through sugar availability. Signaling by the conserved glucose sensor HEXOKINASE1 (HXK1) has been shown to exert both growth-promoting and growth-inhibitory effects depending on the sugar levels, the environmental conditions and the plant species. Here, we used a hxk1 mutant in the Col-0 background to investigate the role of HXK1 during leaf growth in more detail and show that it is affected in both cell proliferation and cell expansion early during leaf development. Furthermore, the hxk1 mutant is less sensitive to sucrose-induced cell proliferation with no significant increase in final leaf growth after transfer to sucrose. Early during leaf development, transfer to sucrose stimulates expression of GLUCOSE-6-PHOSPHATE/PHOSPHATE TRANSPORTER2 (GPT2) and represses chloroplast differentiation. However, in the hxk1 mutant GPT2 expression was still upregulated by transfer to sucrose although chloroplast differentiation was not affected, suggesting that GPT2 is not involved in HXK1-dependent regulation of leaf growth. Finally, using tandem affinity purification of protein complexes from cell cultures, we identified KIN gamma, a protein containing four cystathionine beta-synthase domains, as an interacting protein of HXK1.}},
  author       = {{Van Dingenen, Judith and Vermeersch, Mattias and De Milde, Liesbeth and Hulsmans, Sander and De Winne, Nancy and Van Leene, Jelle and Gonzalez Sanchez, Nathalie and Dhondt, Stijn and De Jaeger, Geert and Rolland, Filip and Inzé, Dirk}},
  issn         = {{0167-4412}},
  journal      = {{PLANT MOLECULAR BIOLOGY}},
  keywords     = {{HEXOKINASE1,Leaf growth,Sucrose,Sink-source,TANDEM AFFINITY PURIFICATION,KINASE COMPLEX,ALTERNATIVE OXIDASE,PLANT-GROWTH,SUGAR,EXPRESSION,THALIANA,GLUCOSE,SENSOR,PROTEINS}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{79--93}},
  title        = {{The role of HEXOKINASE1 in Arabidopsis leaf growth}},
  url          = {{http://doi.org/10.1007/s11103-018-0803-0}},
  volume       = {{99}},
  year         = {{2019}},
}
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