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Hypomethylated drm1 drm2 cmt3 mutant phenotype of Arabidopsis thaliana is related to auxin pathway impairment

(2019) Plant Science. 280. p.383-396
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Abstract
DNA methylation carried out by different methyltransferase classes is a relevant epigenetic modification of DNA which plays a relevant role in the development of eukaryotic organisms. Accordingly, in Arabidopsis thaliana loss of DNA methylation due to combined mutations in genes encoding for DNA methyltransferases causes several developmental abnormalities. The present study describes novel growth disorders in the drm1 drm2 cmt3 triple mutant of Arabidopsis thaliana, defective both in maintenance and de novo DNA methylation, and highlights the correlation between DNA methylation and the auxin hormone pathway. By using an auxin responsive reporter gene, we discovered that auxin accumulation and distribution were affected in the mutant compared to the wild type, from embryo to adult plant stage. In addition, we demonstrated that the defective methylation status also affected the expression of genes that regulate auxin hormone pathways from synthesis to transport and signalling and a direct relationship between differentially expressed auxin-related genes and altered auxin accumulation and distribution in embryo, leaf and root was observed. Finally, we provided evidence of the direct and organ-specific modulation of auxin-related genes through the DNA methylation process.
Keywords
Agronomy and Crop Science, Plant Science, Genetics, General Medicine

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Chicago
Forgione, Ivano, Magdalena Woloszynska, Marianna Pacenza, Adriana Chiappetta, Maria Greco, Fabrizio Araniti, Maria Rosa Abenavoli, Maria Van Lijsebettens, Maria Beatrice Bitonti, and Leonardo Bruno. 2019. “Hypomethylated Drm1 Drm2 Cmt3 Mutant Phenotype of Arabidopsis Thaliana Is Related to Auxin Pathway Impairment.” Plant Science 280: 383–396.
APA
Forgione, I., Woloszynska, M., Pacenza, M., Chiappetta, A., Greco, M., Araniti, F., Abenavoli, M. R., et al. (2019). Hypomethylated drm1 drm2 cmt3 mutant phenotype of Arabidopsis thaliana is related to auxin pathway impairment. Plant Science, 280, 383–396.
Vancouver
1.
Forgione I, Woloszynska M, Pacenza M, Chiappetta A, Greco M, Araniti F, et al. Hypomethylated drm1 drm2 cmt3 mutant phenotype of Arabidopsis thaliana is related to auxin pathway impairment. Plant Science. Elsevier BV; 2019;280:383–96.
MLA
Forgione, Ivano et al. “Hypomethylated Drm1 Drm2 Cmt3 Mutant Phenotype of Arabidopsis Thaliana Is Related to Auxin Pathway Impairment.” Plant Science 280 (2019): 383–396. Print.
@article{8599208,
  abstract     = {DNA methylation carried out by different methyltransferase classes is a relevant epigenetic modification of DNA which plays a relevant role in the development of eukaryotic organisms. Accordingly, in Arabidopsis thaliana loss of DNA methylation due to combined mutations in genes encoding for DNA methyltransferases causes several developmental abnormalities.

The present study describes novel growth disorders in the drm1 drm2 cmt3 triple mutant of Arabidopsis thaliana, defective both in maintenance and de novo DNA methylation, and highlights the correlation between DNA methylation and the auxin hormone pathway. By using an auxin responsive reporter gene, we discovered that auxin accumulation and distribution were affected in the mutant compared to the wild type, from embryo to adult plant stage. In addition, we demonstrated that the defective methylation status also affected the expression of genes that regulate auxin hormone pathways from synthesis to transport and signalling and a direct relationship between differentially expressed auxin-related genes and altered auxin accumulation and distribution in embryo, leaf and root was observed. Finally, we provided evidence of the direct and organ-specific modulation of auxin-related genes through the DNA methylation process.},
  author       = {Forgione, Ivano and Woloszynska, Magdalena and Pacenza, Marianna and Chiappetta, Adriana and Greco, Maria and Araniti, Fabrizio and Abenavoli, Maria Rosa and Van Lijsebettens, Maria and Bitonti, Maria Beatrice and Bruno, Leonardo},
  issn         = {0168-9452},
  journal      = {Plant Science},
  language     = {eng},
  pages        = {383--396},
  publisher    = {Elsevier BV},
  title        = {Hypomethylated drm1 drm2 cmt3 mutant phenotype of Arabidopsis thaliana is related to auxin pathway impairment},
  url          = {http://dx.doi.org/10.1016/j.plantsci.2018.12.029},
  volume       = {280},
  year         = {2019},
}

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