Advanced search
1 file | 1.28 MB

In vivo detection of protein cysteine sulfenylation in plastids

(2019) PLANT JOURNAL. 97(4). p.765-778
Author
Organization
Abstract
Protein cysteine thiols are post‐translationally modified under oxidative stress conditions. Illuminated chloroplasts are one of the important sources of hydrogen peroxide (H2O2) and are highly sensitive to environmental stimuli, yet a comprehensive view of the oxidation‐sensitive chloroplast proteome is still missing. By targeting the sulfenic acid YAP1C‐trapping technology to the plastids of light‐grown Arabidopsis cells, we identified 132 putatively sulfenylated plastid proteins upon H2O2 pulse treatment. Almost half of the sulfenylated proteins are enzymes of the amino acid metabolism. Using metabolomics, we observed a reversible decrease in the levels of the amino acids Ala, Asn, Cys, Gln, Glu, His, Ile, Leu, Lys, Phe, Ser, Thr and Val after H2O2 treatment, which is in line with an anticipated decrease in the levels of the glycolysis and tricarboxylic acid metabolites. Through the identification of an organelle‐tailored proteome, we demonstrated that the subcellular targeting of the YAP1C probe enables us to study in vivo cysteine sulfenylation at the organellar level. All in all, the identification of these oxidation events in plastids revealed that several enzymes of the amino acid metabolism rapidly undergo cysteine oxidation upon oxidative stress.
Keywords
Plant Science, Genetics, Cell Biology

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.28 MB

Citation

Please use this url to cite or link to this publication:

Chicago
De Smet, Barbara, Patrick Willems, Alvaro Daniel Fernández Fernández, Saleh Alseekh, Alisdair R Fernie, Joris Messens, and Frank Van Breusegem. 2019. “In Vivo Detection of Protein Cysteine Sulfenylation in Plastids.” Plant Journal 97 (4): 765–778.
APA
De Smet, B., Willems, P., Fernández Fernández, A. D., Alseekh, S., Fernie, A. R., Messens, J., & Van Breusegem, F. (2019). In vivo detection of protein cysteine sulfenylation in plastids. PLANT JOURNAL, 97(4), 765–778.
Vancouver
1.
De Smet B, Willems P, Fernández Fernández AD, Alseekh S, Fernie AR, Messens J, et al. In vivo detection of protein cysteine sulfenylation in plastids. PLANT JOURNAL. 2019;97(4):765–78.
MLA
De Smet, Barbara et al. “In Vivo Detection of Protein Cysteine Sulfenylation in Plastids.” PLANT JOURNAL 97.4 (2019): 765–778. Print.
@article{8599143,
  abstract     = {Protein cysteine thiols are post\unmatched{2010}translationally modified under oxidative stress conditions. Illuminated chloroplasts are one of the important sources of hydrogen peroxide (H2O2) and are highly sensitive to environmental stimuli, yet a comprehensive view of the oxidation\unmatched{2010}sensitive chloroplast proteome is still missing. By targeting the sulfenic acid YAP1C\unmatched{2010}trapping technology to the plastids of light\unmatched{2010}grown Arabidopsis cells, we identified 132 putatively sulfenylated plastid proteins upon H2O2 pulse treatment. Almost half of the sulfenylated proteins are enzymes of the amino acid metabolism. Using metabolomics, we observed a reversible decrease in the levels of the amino acids Ala, Asn, Cys, Gln, Glu, His, Ile, Leu, Lys, Phe, Ser, Thr and Val after H2O2 treatment, which is in line with an anticipated decrease in the levels of the glycolysis and tricarboxylic acid metabolites. Through the identification of an organelle\unmatched{2010}tailored proteome, we demonstrated that the subcellular targeting of the YAP1C probe enables us to study in vivo cysteine sulfenylation at the organellar level. All in all, the identification of these oxidation events in plastids revealed that several enzymes of the amino acid metabolism rapidly undergo cysteine oxidation upon oxidative stress.},
  author       = {De Smet, Barbara and Willems, Patrick and Fern{\'a}ndez Fern{\'a}ndez, Alvaro Daniel and Alseekh, Saleh and Fernie, Alisdair R and Messens, Joris and Van Breusegem, Frank},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  language     = {eng},
  number       = {4},
  pages        = {765--778},
  title        = {In vivo detection of protein cysteine sulfenylation in plastids},
  url          = {http://dx.doi.org/10.1111/tpj.14146},
  volume       = {97},
  year         = {2019},
}

Altmetric
View in Altmetric