Advanced search
1 file | 1.75 MB

Mitochondrial function modulates touch signalling in Arabidopsis thaliana

(2019) PLANT JOURNAL. 97(4). p.623-645
Author
Organization
Abstract
Plants respond to short- and long-term mechanical stimuli, via altered transcript abundance and growth respectively. Jasmonate, gibberellic acid and calcium have been implicated in mediating responses to mechanical stimuli. Previously it has been shown that the transcript abundance for the outer mitochondrial membrane protein of 66 kDa (OM66), is induced several fold after 30 min in response to touch. Therefore, the effect of mitochondrial function on the response to mechanical stimulation by touch at 30 min was investigated. Twenty-five mutants targeting mitochondrial function or regulators revealed that all affected the touch transcriptome. Double and triple mutants revealed synergistic or antagonistic effects following the observed responses in the single mutants. Changes in the touch-responsive transcriptome were localised, recurring with repeated rounds of stimulus. The gene expression kinetics after repeated touch were complex, displaying five distinct patterns. These transcriptomic responses were altered by some regulators of mitochondrial retrograde signalling, such as cyclic dependent protein kinase E1, a kinase protein in the mediator complex, and KIN10 (SnRK1 - sucrose non-fermenting related protein kinase 1), revealing an overlap between the touch response and mitochondrial stress signalling and alternative mitochondrial metabolic pathways. Regulatory network analyses revealed touch-induced stress responses and suppressed growth and biosynthetic processes. Interaction with the phytohormone signalling pathways indicated that ethylene and gibberellic acid had the greatest effect. Hormone measurements revealed that mutations of genes that encoded mitochondrial proteins altered hormone concentrations. Mitochondrial function modulates touch-induced changes in gene expression directly through altered regulatory networks, and indirectly via altering hormonal levels.
Keywords
Plant Science, Genetics, Cell Biology

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.75 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Xu, Yue, Oliver Berkowitz, Reena Narsai, Inge De Clercq, Michelle Hooi, Bulone Vincent, Frank Van Breusegem, James Whelan, and Yan Wang. 2019. “Mitochondrial Function Modulates Touch Signalling in Arabidopsis Thaliana.” Plant Journal 97 (4): 623–645.
APA
Xu, Y., Berkowitz, O., Narsai, R., De Clercq, I., Hooi, M., Vincent, B., Van Breusegem, F., et al. (2019). Mitochondrial function modulates touch signalling in Arabidopsis thaliana. PLANT JOURNAL, 97(4), 623–645.
Vancouver
1.
Xu Y, Berkowitz O, Narsai R, De Clercq I, Hooi M, Vincent B, et al. Mitochondrial function modulates touch signalling in Arabidopsis thaliana. PLANT JOURNAL. 2019;97(4):623–45.
MLA
Xu, Yue et al. “Mitochondrial Function Modulates Touch Signalling in Arabidopsis Thaliana.” PLANT JOURNAL 97.4 (2019): 623–645. Print.
@article{8599137,
  abstract     = {Plants respond to short- and long-term mechanical stimuli, via altered transcript abundance and growth respectively. Jasmonate, gibberellic acid and calcium have been implicated in mediating responses to mechanical stimuli. Previously it has been shown that the transcript abundance for the outer mitochondrial membrane protein of 66 kDa (OM66), is induced several fold after 30 min in response to touch. Therefore, the effect of mitochondrial function on the response to mechanical stimulation by touch at 30 min was investigated. Twenty-five mutants targeting mitochondrial function or regulators revealed that all affected the touch transcriptome. Double and triple mutants revealed synergistic or antagonistic effects following the observed responses in the single mutants. Changes in the touch-responsive transcriptome were localised, recurring with repeated rounds of stimulus. The gene expression kinetics after repeated touch were complex, displaying five distinct patterns. These transcriptomic responses were altered by some regulators of mitochondrial retrograde signalling, such as cyclic dependent protein kinase E1, a kinase protein in the mediator complex, and KIN10 (SnRK1 - sucrose non-fermenting related protein kinase 1), revealing an overlap between the touch response and mitochondrial stress signalling and alternative mitochondrial metabolic pathways. Regulatory network analyses revealed touch-induced stress responses and suppressed growth and biosynthetic processes. Interaction with the phytohormone signalling pathways indicated that ethylene and gibberellic acid had the greatest effect. Hormone measurements revealed that mutations of genes that encoded mitochondrial proteins altered hormone concentrations. Mitochondrial function modulates touch-induced changes in gene expression directly through altered regulatory networks, and indirectly via altering hormonal levels.},
  author       = {Xu, Yue and Berkowitz, Oliver and Narsai, Reena and De Clercq, Inge and Hooi, Michelle and Vincent, Bulone and Van Breusegem, Frank and Whelan, James and Wang, Yan},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  language     = {eng},
  number       = {4},
  pages        = {623--645},
  title        = {Mitochondrial function modulates touch signalling in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1111/tpj.14183},
  volume       = {97},
  year         = {2019},
}

Altmetric
View in Altmetric