Advanced search
1 file | 3.12 MB

Interactive and noninteractive roles of histone H2B monoubiquitination and H3K36 methylation in the regulation of active gene transcription and control of plant growth and development

(2019) NEW PHYTOLOGIST. 221(2). p.1101-1116
Author
Organization
Abstract
Covalent modifications of histones are essential to control a wide range of processes during development and adaptation to environmental changes. With the establishment of reference epigenomes, patterns of histone modifications were correlated with transcriptionally active or silenced genes. These patterns imply the need for the precise and dynamic coordination of different histone-modifying enzymes to control transcription at a given gene. Classically, the influence of these enzymes on gene expression is examined separately and their interplays rarely established. In Arabidopsis, HISTONE MONOUBIQUITINATION2 (HUB2) mediates H2B monoubiquitination (H2Bub1), whereas SET DOMAIN GROUP8 (SDG8) catalyzes H3 lysine 36 trimethylation (H3K36me3). In this work, we crossed hub2 with sdg8 mutants to elucidate their functional relationships. Despite similar phenotypic defects, sdg8 and hub2 mutations broadly affect genome transcription and plant growth and development synergistically. Also, whereas H3K4 methylation appears largely dependent on H2Bub1, H3K36me3 and H2Bub1 modifications mutually reinforce each other at some flowering time genes. In addition, SDG8 and HUB2 jointly antagonize the increase of the H3K27me3 repressive mark. Collectively, our data provide an important insight into the interplay between histone marks and highlight their interactive complexity in regulating chromatin landscape which might be necessary to fine-tune transcription and ensure plant developmental plasticity.
Keywords
Arabidopsis thaliana, flowering time, histone crosstalk, histone methylation, histone monoubiquitination, transcription regulation, FLOWERING-LOCUS-C, CELL-CYCLE REGULATION, REPRESSIVE COMPLEX 2, WINTER-ANNUAL HABIT, CHROMATIN-STRUCTURE, YEAST BRE1, ARABIDOPSIS, H3, DOMAIN, UBIQUITYLATION

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.12 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Zhao, Wei, Pia Neyt, Maria Van Lijsebettens, Wen-Hui Shen, and Alexandre Berr. 2019. “Interactive and Noninteractive Roles of Histone H2B Monoubiquitination and H3K36 Methylation in the Regulation of Active Gene Transcription and Control of Plant Growth and Development.” New Phytologist 221 (2): 1101–1116.
APA
Zhao, W., Neyt, P., Van Lijsebettens, M., Shen, W.-H., & Berr, A. (2019). Interactive and noninteractive roles of histone H2B monoubiquitination and H3K36 methylation in the regulation of active gene transcription and control of plant growth and development. NEW PHYTOLOGIST, 221(2), 1101–1116.
Vancouver
1.
Zhao W, Neyt P, Van Lijsebettens M, Shen W-H, Berr A. Interactive and noninteractive roles of histone H2B monoubiquitination and H3K36 methylation in the regulation of active gene transcription and control of plant growth and development. NEW PHYTOLOGIST. 2019;221(2):1101–16.
MLA
Zhao, Wei et al. “Interactive and Noninteractive Roles of Histone H2B Monoubiquitination and H3K36 Methylation in the Regulation of Active Gene Transcription and Control of Plant Growth and Development.” NEW PHYTOLOGIST 221.2 (2019): 1101–1116. Print.
@article{8599114,
  abstract     = {Covalent modifications of histones are essential to control a wide range of processes during development and adaptation to environmental changes. With the establishment of reference epigenomes, patterns of histone modifications were correlated with transcriptionally active or silenced genes. These patterns imply the need for the precise and dynamic coordination of different histone-modifying enzymes to control transcription at a given gene. Classically, the influence of these enzymes on gene expression is examined separately and their interplays rarely established. In Arabidopsis, HISTONE MONOUBIQUITINATION2 (HUB2) mediates H2B monoubiquitination (H2Bub1), whereas SET DOMAIN GROUP8 (SDG8) catalyzes H3 lysine 36 trimethylation (H3K36me3). In this work, we crossed hub2 with sdg8 mutants to elucidate their functional relationships. Despite similar phenotypic defects, sdg8 and hub2 mutations broadly affect genome transcription and plant growth and development synergistically. Also, whereas H3K4 methylation appears largely dependent on H2Bub1, H3K36me3 and H2Bub1 modifications mutually reinforce each other at some flowering time genes. In addition, SDG8 and HUB2 jointly antagonize the increase of the H3K27me3 repressive mark. Collectively, our data provide an important insight into the interplay between histone marks and highlight their interactive complexity in regulating chromatin landscape which might be necessary to fine-tune transcription and ensure plant developmental plasticity.},
  author       = {Zhao, Wei and Neyt, Pia and Van Lijsebettens, Maria and Shen, Wen-Hui and Berr, Alexandre},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  number       = {2},
  pages        = {1101--1116},
  title        = {Interactive and noninteractive roles of histone H2B monoubiquitination and H3K36 methylation in the regulation of active gene transcription and control of plant growth and development},
  url          = {http://dx.doi.org/10.1111/nph.15418},
  volume       = {221},
  year         = {2019},
}

Altmetric
View in Altmetric
Web of Science
Times cited: