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Ectopic activation of cortical cell division during the accommodation of arbuscular mycorrhizal fungi

(2019) NEW PHYTOLOGIST. 221(2). p.1036-1048
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Abstract
Arbuscular mycorrhizas (AMs) between plants and soil fungi are widespread symbioses with a major role in soil nutrient uptake. In this study we investigated the induction of root cortical cell division during AM colonization by combining morphometric and gene expression analyses with promoter activation and protein localization studies of the cell-plate-associated exocytic marker TPLATE. Our results show that TPLATE promoter is activated in colonized cells of the root cortex where we also observed the appearance of cells that are half the size of the surrounding cells. Furthermore, TPLATE-green fluorescent protein recruitment to developing cell plates highlighted ectopic cell division events in the inner root cortex during early AM colonization. Lastly, transcripts of TPLATE, KNOLLE and Cyclinlike 1 (CYC1) are all upregulated in the same context, alongside endocytic markers Adaptor-Related Protein complex 2 alpha 1 subunit (AP2A1) and Clathrin Heavy Chain 2 (CHC2), known to be active during cell plate formation. This pattern of gene expression was recorded in wild-type Medicago truncatula roots, but not in a common symbiotic signalling pathway mutant where fungal colonization is blocked at the epidermal level. Altogether, these results suggest the activation of cell-division-related mechanisms by AM hosts during the accommodation of the symbiotic fungus.
Keywords
MEDICAGO-TRUNCATULA, AGROBACTERIUM-RHIZOGENES, PERIARBUSCULAR MEMBRANE, PLATE FORMATION, ROOT HAIRS, ENDOCYTOSIS, LEGUME, INFECTION, SYMBIOSIS, REVEALS, arbuscular mycorrhiza, cell division, Daucus carota, endocytosis, live, confocal imaging, Lotus japonicus, Medicago truncatula, symbiosis

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Chicago
Russo, Giulia, Gennaro Carotenuto, Valentina Fiorilli, Veronica Volpe, Marco Chiapello, Daniël Van Damme, and Andrea Genre. 2019. “Ectopic Activation of Cortical Cell Division During the Accommodation of Arbuscular Mycorrhizal Fungi.” New Phytologist 221 (2): 1036–1048.
APA
Russo, Giulia, Carotenuto, G., Fiorilli, V., Volpe, V., Chiapello, M., Van Damme, D., & Genre, A. (2019). Ectopic activation of cortical cell division during the accommodation of arbuscular mycorrhizal fungi. NEW PHYTOLOGIST, 221(2), 1036–1048.
Vancouver
1.
Russo G, Carotenuto G, Fiorilli V, Volpe V, Chiapello M, Van Damme D, et al. Ectopic activation of cortical cell division during the accommodation of arbuscular mycorrhizal fungi. NEW PHYTOLOGIST. 2019;221(2):1036–48.
MLA
Russo, Giulia et al. “Ectopic Activation of Cortical Cell Division During the Accommodation of Arbuscular Mycorrhizal Fungi.” NEW PHYTOLOGIST 221.2 (2019): 1036–1048. Print.
@article{8599112,
  abstract     = {Arbuscular mycorrhizas (AMs) between plants and soil fungi are widespread symbioses with a major role in soil nutrient uptake. In this study we investigated the induction of root cortical cell division during AM colonization by combining morphometric and gene expression analyses with promoter activation and protein localization studies of the cell-plate-associated exocytic marker TPLATE. Our results show that TPLATE promoter is activated in colonized cells of the root cortex where we also observed the appearance of cells that are half the size of the surrounding cells. Furthermore, TPLATE-green fluorescent protein recruitment to developing cell plates highlighted ectopic cell division events in the inner root cortex during early AM colonization. Lastly, transcripts of TPLATE, KNOLLE and Cyclinlike 1 (CYC1) are all upregulated in the same context, alongside endocytic markers Adaptor-Related Protein complex 2 alpha 1 subunit (AP2A1) and Clathrin Heavy Chain 2 (CHC2), known to be active during cell plate formation. This pattern of gene expression was recorded in wild-type Medicago truncatula roots, but not in a common symbiotic signalling pathway mutant where fungal colonization is blocked at the epidermal level. Altogether, these results suggest the activation of cell-division-related mechanisms by AM hosts during the accommodation of the symbiotic fungus.},
  author       = {Russo, Giulia and Carotenuto, Gennaro and Fiorilli, Valentina and Volpe, Veronica and Chiapello, Marco and Van Damme, Dani{\"e}l and Genre, Andrea},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  language     = {eng},
  number       = {2},
  pages        = {1036--1048},
  title        = {Ectopic activation of cortical cell division during the accommodation of arbuscular mycorrhizal fungi},
  url          = {http://dx.doi.org/10.1111/nph.15398},
  volume       = {221},
  year         = {2019},
}

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