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Comparative study of the differential cell death protecting effect of various ROS scavengers

(2019) BIOLOGICAL CHEMISTRY. 400(2). p.149-160
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Abstract
Oxidative stress has for a long time been associated with cell death, especially classical necrosis, however, its role in other cell death pathways is less clear. Here, we evaluated in a comparative way, the effect of four different reactive oxygen species (ROS) scavengers, N-acetyl-L-cysteine (NAC), alpha-tocopherol and two superoxide dismutase mimetics, n(III) tetrakis(4-benzoic acid) porphyrin chloride, and 4-hydroxy-2,2,6,6-tetramethyl-piperidine 1-oxyl (Tempol), in four different cell death models, including menadione-triggered necrosis, staurosporine-induced apoptosis and tumor necrosis factor (TNF)-induced apoptosis and necroptosis. While menadione-triggered necrosis was completely prevented by the classical ROS scavenger NAC and to a substantial amount by the other scavengers, ROS targeting was found to have a marginal effect on the other cell death modalities investigated. Despite its side-effects at higher concentrations, Tempol was able to substantially prevent TNF-induced apoptosis and to a somewhat lesser extent TNF-induced necroptosis. However, this seems to be separated from its ROS-scavenging function.
Keywords
FACTOR-INDUCED NECROSIS, NF-KAPPA-B, MOLECULAR-MECHANISMS, INDUCED, APOPTOSIS, OXIDATIVE STRESS, MITOCHONDRIAL, INHIBITION, ACETYLCYSTEINE, CYTOTOXICITY, ANTIOXIDANTS, apoptosis, cell death, necroptosis, necrosis, ROS, ROS scavenger, Tempol

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MLA
Kavcic, Nezka et al. “Comparative Study of the Differential Cell Death Protecting Effect of Various ROS Scavengers.” BIOLOGICAL CHEMISTRY 400.2 (2019): 149–160. Print.
APA
Kavcic, N., Pegan, K., Vandenabeele, P., & Turk, B. (2019). Comparative study of the differential cell death protecting effect of various ROS scavengers. BIOLOGICAL CHEMISTRY, 400(2), 149–160.
Chicago author-date
Kavcic, Nezka, Katarina Pegan, Peter Vandenabeele, and Boris Turk. 2019. “Comparative Study of the Differential Cell Death Protecting Effect of Various ROS Scavengers.” Biological Chemistry 400 (2): 149–160.
Chicago author-date (all authors)
Kavcic, Nezka, Katarina Pegan, Peter Vandenabeele, and Boris Turk. 2019. “Comparative Study of the Differential Cell Death Protecting Effect of Various ROS Scavengers.” Biological Chemistry 400 (2): 149–160.
Vancouver
1.
Kavcic N, Pegan K, Vandenabeele P, Turk B. Comparative study of the differential cell death protecting effect of various ROS scavengers. BIOLOGICAL CHEMISTRY. 2019;400(2):149–60.
IEEE
[1]
N. Kavcic, K. Pegan, P. Vandenabeele, and B. Turk, “Comparative study of the differential cell death protecting effect of various ROS scavengers,” BIOLOGICAL CHEMISTRY, vol. 400, no. 2, pp. 149–160, 2019.
@article{8599049,
  abstract     = {Oxidative stress has for a long time been associated with cell death, especially classical necrosis, however, its role in other cell death pathways is less clear. Here, we evaluated in a comparative way, the effect of four different reactive oxygen species (ROS) scavengers, N-acetyl-L-cysteine (NAC), alpha-tocopherol and two superoxide dismutase mimetics, n(III) tetrakis(4-benzoic acid) porphyrin chloride, and 4-hydroxy-2,2,6,6-tetramethyl-piperidine 1-oxyl (Tempol), in four different cell death models, including menadione-triggered necrosis, staurosporine-induced apoptosis and tumor necrosis factor (TNF)-induced apoptosis and necroptosis. While menadione-triggered necrosis was completely prevented by the classical ROS scavenger NAC and to a substantial amount by the other scavengers, ROS targeting was found to have a marginal effect on the other cell death modalities investigated. Despite its side-effects at higher concentrations, Tempol was able to substantially prevent TNF-induced apoptosis and to a somewhat lesser extent TNF-induced necroptosis. However, this seems to be separated from its ROS-scavenging function.},
  author       = {Kavcic, Nezka and Pegan, Katarina and Vandenabeele, Peter and Turk, Boris},
  issn         = {1431-6730},
  journal      = {BIOLOGICAL CHEMISTRY},
  keywords     = {FACTOR-INDUCED NECROSIS,NF-KAPPA-B,MOLECULAR-MECHANISMS,INDUCED,APOPTOSIS,OXIDATIVE STRESS,MITOCHONDRIAL,INHIBITION,ACETYLCYSTEINE,CYTOTOXICITY,ANTIOXIDANTS,apoptosis,cell death,necroptosis,necrosis,ROS,ROS scavenger,Tempol},
  language     = {eng},
  number       = {2},
  pages        = {149--160},
  title        = {Comparative study of the differential cell death protecting effect of various ROS scavengers},
  url          = {http://dx.doi.org/10.1515/hsz-2017-0317},
  volume       = {400},
  year         = {2019},
}

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