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Identification of RNAi-related genes and transgenerational efficiency of RNAi in Artemia franciscana

(2019) AQUACULTURE. 501. p.285-292
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Abstract
Artemia and the RNA interference technique were used in research to explore the function of genes in crustaceans. In this study, we annotated and characterized nine putative genes involved in RNA interference in Artemia franciscana including two Dicer, three Argonauts, two dsRNA binding proteins, Drosha and Sid-1 together with evidence of Pasha and Exportin-5. The phylogenetic results indicated the identity of these genes and revealed that genes homologous to those in both the siRNA and miRNA pathway in arthropods are also present in Artemia franciscana. This study provides the first look at the core genes of the RNAi machinery of A. franciscana. Additionally, egg-sac microinjection was used to generate the desired RNAi phenotype. The high numbers of nauplii with the desired phenotypes obtained from injected maternal A. franciscana (both from the first and second brood) demonstrated the reliability of the egg-sac microinjection method, providing a methodology to study gene function on high numbers of individuals be treating the parental generation.
Keywords
RNA interference (RNAi), Artemia franciscana, Dicer, Argonaute, dsRNA, DOUBLE-STRANDED-RNA, RIBOSOMAL S6 KINASE, PENAEUS-MONODON, DISTINCT ROLES, PARENTAL RNAI, BRINE SHRIMP, HUMAN DICER, PROTEIN, DSRNA, TRBP

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MLA
Nguyen, Viet Dung, et al. “Identification of RNAi-Related Genes and Transgenerational Efficiency of RNAi in Artemia Franciscana.” AQUACULTURE, vol. 501, 2019, pp. 285–92, doi:10.1016/j.aquaculture.2018.09.042.
APA
Nguyen, V. D., Christiaens, O., Van Bao Duy, L., De Vos, S., MacRae, T. H., Smagghe, G., & Bossier, P. (2019). Identification of RNAi-related genes and transgenerational efficiency of RNAi in Artemia franciscana. AQUACULTURE, 501, 285–292. https://doi.org/10.1016/j.aquaculture.2018.09.042
Chicago author-date
Nguyen, Viet Dung, Olivier Christiaens, Le Van Bao Duy, Stephanie De Vos, Thomas H MacRae, Guy Smagghe, and Peter Bossier. 2019. “Identification of RNAi-Related Genes and Transgenerational Efficiency of RNAi in Artemia Franciscana.” AQUACULTURE 501: 285–92. https://doi.org/10.1016/j.aquaculture.2018.09.042.
Chicago author-date (all authors)
Nguyen, Viet Dung, Olivier Christiaens, Le Van Bao Duy, Stephanie De Vos, Thomas H MacRae, Guy Smagghe, and Peter Bossier. 2019. “Identification of RNAi-Related Genes and Transgenerational Efficiency of RNAi in Artemia Franciscana.” AQUACULTURE 501: 285–292. doi:10.1016/j.aquaculture.2018.09.042.
Vancouver
1.
Nguyen VD, Christiaens O, Van Bao Duy L, De Vos S, MacRae TH, Smagghe G, et al. Identification of RNAi-related genes and transgenerational efficiency of RNAi in Artemia franciscana. AQUACULTURE. 2019;501:285–92.
IEEE
[1]
V. D. Nguyen et al., “Identification of RNAi-related genes and transgenerational efficiency of RNAi in Artemia franciscana,” AQUACULTURE, vol. 501, pp. 285–292, 2019.
@article{8598983,
  abstract     = {{Artemia and the RNA interference technique were used in research to explore the function of genes in crustaceans. In this study, we annotated and characterized nine putative genes involved in RNA interference in Artemia franciscana including two Dicer, three Argonauts, two dsRNA binding proteins, Drosha and Sid-1 together with evidence of Pasha and Exportin-5. The phylogenetic results indicated the identity of these genes and revealed that genes homologous to those in both the siRNA and miRNA pathway in arthropods are also present in Artemia franciscana. This study provides the first look at the core genes of the RNAi machinery of A. franciscana. Additionally, egg-sac microinjection was used to generate the desired RNAi phenotype. The high numbers of nauplii with the desired phenotypes obtained from injected maternal A. franciscana (both from the first and second brood) demonstrated the reliability of the egg-sac microinjection method, providing a methodology to study gene function on high numbers of individuals be treating the parental generation.}},
  author       = {{Nguyen, Viet Dung and Christiaens, Olivier and Van Bao Duy, Le and De Vos, Stephanie and MacRae, Thomas H and Smagghe, Guy and Bossier, Peter}},
  issn         = {{0044-8486}},
  journal      = {{AQUACULTURE}},
  keywords     = {{RNA interference (RNAi),Artemia franciscana,Dicer,Argonaute,dsRNA,DOUBLE-STRANDED-RNA,RIBOSOMAL S6 KINASE,PENAEUS-MONODON,DISTINCT ROLES,PARENTAL RNAI,BRINE SHRIMP,HUMAN DICER,PROTEIN,DSRNA,TRBP}},
  language     = {{eng}},
  pages        = {{285--292}},
  title        = {{Identification of RNAi-related genes and transgenerational efficiency of RNAi in Artemia franciscana}},
  url          = {{http://dx.doi.org/10.1016/j.aquaculture.2018.09.042}},
  volume       = {{501}},
  year         = {{2019}},
}

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