Advanced search
1 file | 1.20 MB Add to list

Importance of choice of materials and methods in PD-L1 and TIL assessment in oropharyngeal squamous cell carcinoma

(2018) HISTOPATHOLOGY. 73(3). p.500-509
Author
Organization
Abstract
Aims: A great deal of research is being conducted into PD-L1 immunohistochemistry (IHC) and tumour-infiltrating lymphocytes (TILs) as predictive or prognostic biomarkers for immunotherapy, although several practical issues exist concerning their assessment. The aim of this research was therefore to assess the importance of choice of materials and methods in PD-L1 and TILs scoring in oropharyngeal squamous cell carcinoma (OSCC). Methods and results: IHC for PD-L1 (SP142 and 22C3 clone) and TILs subtyping was performed on formalin-fixed paraffin-embedded tissue slides (biopsy, resection and/or lymph nodes specimens) of 99 patients with OSCC. A comparative analysis of PD-L1 and TILs scoring was made between different types of tissue specimens, between different PD-L1 clones, between TILs and different subsets of TILs and between the quantitative and semiquantitative assessments. PD-L1 scoring resulted in fair to moderate agreement for 22C3 and SP142 between various tissue specimens, with higher agreement at higher cut-off values, and in moderate agreement for 22C3 versus SP142. Evaluation by four independent observers proved substantial inter-rater agreement for both clones with high consistency in their ratings. Moderate agreement was observed for TILs and TILs subsets for the comparison between biopsy and resection. Lastly, strong correlations were found between quantitative and semiquantitative assessment for all PD-L1 and TILs scores. Conclusions: Our results highlight the challenges associated with the evaluation of PD-L1 and TILs in OSCC. Further research is warranted to evaluate the use of these biomarkers in order to allow implementation of PD-L1 and TILs infiltrate as biomarkers in daily clinical practice.
Keywords
TUMOR-INFILTRATING LYMPHOCYTES, LUNG-CANCER, STANDARDIZED METHOD, SOLID, TUMORS, EXPRESSION, HEAD, NECK, IMMUNOHISTOCHEMISTRY, BIOMARKERS, IHC, biomarker, methodology, oropharyngeal squamous cell carcinoma, PD-L1, tumour-infiltrating lymphocytes

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.20 MB

Citation

Please use this url to cite or link to this publication:

MLA
De Meulenaere, Astrid et al. “Importance of Choice of Materials and Methods in PD-L1 and TIL Assessment in Oropharyngeal Squamous Cell Carcinoma.” HISTOPATHOLOGY 73.3 (2018): 500–509. Print.
APA
De Meulenaere, A., Vermassen, T., Creytens, D., ASPESLAGH, S., Deron, P., Duprez, F., Rottey, S., et al. (2018). Importance of choice of materials and methods in PD-L1 and TIL assessment in oropharyngeal squamous cell carcinoma. HISTOPATHOLOGY, 73(3), 500–509.
Chicago author-date
De Meulenaere, Astrid, Tijl Vermassen, David Creytens, SANDRINE ASPESLAGH, Philippe Deron, Fréderic Duprez, Sylvie Rottey, Jo Van Dorpe, and Liesbeth Ferdinande. 2018. “Importance of Choice of Materials and Methods in PD-L1 and TIL Assessment in Oropharyngeal Squamous Cell Carcinoma.” Histopathology 73 (3): 500–509.
Chicago author-date (all authors)
De Meulenaere, Astrid, Tijl Vermassen, David Creytens, SANDRINE ASPESLAGH, Philippe Deron, Fréderic Duprez, Sylvie Rottey, Jo Van Dorpe, and Liesbeth Ferdinande. 2018. “Importance of Choice of Materials and Methods in PD-L1 and TIL Assessment in Oropharyngeal Squamous Cell Carcinoma.” Histopathology 73 (3): 500–509.
Vancouver
1.
De Meulenaere A, Vermassen T, Creytens D, ASPESLAGH S, Deron P, Duprez F, et al. Importance of choice of materials and methods in PD-L1 and TIL assessment in oropharyngeal squamous cell carcinoma. HISTOPATHOLOGY. 2018;73(3):500–9.
IEEE
[1]
A. De Meulenaere et al., “Importance of choice of materials and methods in PD-L1 and TIL assessment in oropharyngeal squamous cell carcinoma,” HISTOPATHOLOGY, vol. 73, no. 3, pp. 500–509, 2018.
@article{8593865,
  abstract     = {Aims: A great deal of research is being conducted into PD-L1 immunohistochemistry (IHC) and tumour-infiltrating lymphocytes (TILs) as predictive or prognostic biomarkers for immunotherapy, although several practical issues exist concerning their assessment. The aim of this research was therefore to assess the importance of choice of materials and methods in PD-L1 and TILs scoring in oropharyngeal squamous cell carcinoma (OSCC). 
Methods and results: IHC for PD-L1 (SP142 and 22C3 clone) and TILs subtyping was performed on formalin-fixed paraffin-embedded tissue slides (biopsy, resection and/or lymph nodes specimens) of 99 patients with OSCC. A comparative analysis of PD-L1 and TILs scoring was made between different types of tissue specimens, between different PD-L1 clones, between TILs and different subsets of TILs and between the quantitative and semiquantitative assessments. PD-L1 scoring resulted in fair to moderate agreement for 22C3 and SP142 between various tissue specimens, with higher agreement at higher cut-off values, and in moderate agreement for 22C3 versus SP142. Evaluation by four independent observers proved substantial inter-rater agreement for both clones with high consistency in their ratings. Moderate agreement was observed for TILs and TILs subsets for the comparison between biopsy and resection. Lastly, strong correlations were found between quantitative and semiquantitative assessment for all PD-L1 and TILs scores. 
Conclusions: Our results highlight the challenges associated with the evaluation of PD-L1 and TILs in OSCC. Further research is warranted to evaluate the use of these biomarkers in order to allow implementation of PD-L1 and TILs infiltrate as biomarkers in daily clinical practice.},
  author       = {De Meulenaere, Astrid and Vermassen, Tijl and Creytens, David and ASPESLAGH, SANDRINE and Deron, Philippe and Duprez, Fréderic and Rottey, Sylvie and Van Dorpe, Jo and Ferdinande, Liesbeth},
  issn         = {0309-0167},
  journal      = {HISTOPATHOLOGY},
  keywords     = {TUMOR-INFILTRATING LYMPHOCYTES,LUNG-CANCER,STANDARDIZED METHOD,SOLID,TUMORS,EXPRESSION,HEAD,NECK,IMMUNOHISTOCHEMISTRY,BIOMARKERS,IHC,biomarker,methodology,oropharyngeal squamous cell carcinoma,PD-L1,tumour-infiltrating lymphocytes},
  language     = {eng},
  number       = {3},
  pages        = {500--509},
  title        = {Importance of choice of materials and methods in PD-L1 and TIL assessment in oropharyngeal squamous cell carcinoma},
  url          = {http://dx.doi.org/10.1111/his.13650},
  volume       = {73},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: