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The Salmonella Enteritidis TolC outer membrane channel is essential for egg white survival

Ruth Raspoet (UGent) , Venessa Eeckhaut (UGent) , Karen Vermeulen (UGent) , Lina De Smet (UGent) , Yi Wen (UGent) , K Nishino, Freddy Haesebrouck (UGent) , Richard Ducatelle (UGent) , Bart Devreese (UGent) and Filip Van Immerseel (UGent)
(2019) POULTRY SCIENCE. 98(5). p.2281-2289
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Abstract
Salmonella Enteritidis has developed the potential to contaminate eggs by surviving in the antimicrobial environment of the hen's egg white. This has led to a worldwide pandemic of foodborne salmonellosis infections in humans due to the consumption of contaminated eggs and egg-derived products. The molecular mechanisms of Salmonella Enteritidis egg white survival are not fully clear. Using in vivo expression technology and promoter-reporter fusions we showed that the promoter of the tolC gene, encoding the TolC outer membrane channel that is used by multidrug efflux pumps to export harmful molecules and to secrete bacterial products, is activated by egg white at the chicken body temperature. Using a Salmonella Enteritidis tolC deletion mutant we showed that TolC has an important role in egg white survival. Chromatographic separation techniques and subsequent testing of antimicrobial activities of separated egg white fractions led to the identification of ovotransferrin as the egg white antimicrobial factor which is capable of inhibiting growth of a tolC deletion strain but not the wild type strain. We provide evidence that TolC protects Salmonella Enteritidis against ovotransferrin-mediated growth inhibition in egg white.
Keywords
Salmonella Enteritidis, TolC, multidrug efflux pumps, egg white, ovotransferrin, ENTERICA SEROVAR ENTERITIDIS, MULTIDRUG EFFLUX SYSTEMS, REPRODUCTIVE-TRACT, CHICKEN, COLONIZATION, RESISTANCE, CONTAMINATION, GENES, HENS, BIOSYNTHESIS

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MLA
Raspoet, Ruth, et al. “The Salmonella Enteritidis TolC Outer Membrane Channel Is Essential for Egg White Survival.” POULTRY SCIENCE, vol. 98, no. 5, 2019, pp. 2281–89.
APA
Raspoet, R., Eeckhaut, V., Vermeulen, K., De Smet, L., Wen, Y., Nishino, K., … Van Immerseel, F. (2019). The Salmonella Enteritidis TolC outer membrane channel is essential for egg white survival. POULTRY SCIENCE, 98(5), 2281–2289.
Chicago author-date
Raspoet, Ruth, Venessa Eeckhaut, Karen Vermeulen, Lina De Smet, Yi Wen, K Nishino, Freddy Haesebrouck, Richard Ducatelle, Bart Devreese, and Filip Van Immerseel. 2019. “The Salmonella Enteritidis TolC Outer Membrane Channel Is Essential for Egg White Survival.” POULTRY SCIENCE 98 (5): 2281–89.
Chicago author-date (all authors)
Raspoet, Ruth, Venessa Eeckhaut, Karen Vermeulen, Lina De Smet, Yi Wen, K Nishino, Freddy Haesebrouck, Richard Ducatelle, Bart Devreese, and Filip Van Immerseel. 2019. “The Salmonella Enteritidis TolC Outer Membrane Channel Is Essential for Egg White Survival.” POULTRY SCIENCE 98 (5): 2281–2289.
Vancouver
1.
Raspoet R, Eeckhaut V, Vermeulen K, De Smet L, Wen Y, Nishino K, et al. The Salmonella Enteritidis TolC outer membrane channel is essential for egg white survival. POULTRY SCIENCE. 2019;98(5):2281–9.
IEEE
[1]
R. Raspoet et al., “The Salmonella Enteritidis TolC outer membrane channel is essential for egg white survival,” POULTRY SCIENCE, vol. 98, no. 5, pp. 2281–2289, 2019.
@article{8592998,
  abstract     = {Salmonella Enteritidis has developed the potential to contaminate eggs by surviving in the antimicrobial environment of the hen's egg white. This has led to a worldwide pandemic of foodborne salmonellosis infections in humans due to the consumption of contaminated eggs and egg-derived products. The molecular mechanisms of Salmonella Enteritidis egg white survival are not fully clear. Using in vivo expression technology and promoter-reporter fusions we showed that the promoter of the tolC gene, encoding the TolC outer membrane channel that is used by multidrug efflux pumps to export harmful molecules and to secrete bacterial products, is activated by egg white at the chicken body temperature. Using a Salmonella Enteritidis tolC deletion mutant we showed that TolC has an important role in egg white survival. Chromatographic separation techniques and subsequent testing of antimicrobial activities of separated egg white fractions led to the identification of ovotransferrin as the egg white antimicrobial factor which is capable of inhibiting growth of a tolC deletion strain but not the wild type strain. We provide evidence that TolC protects Salmonella Enteritidis against ovotransferrin-mediated growth inhibition in egg white.},
  author       = {Raspoet, Ruth and Eeckhaut, Venessa and Vermeulen, Karen and De Smet, Lina and Wen, Yi and Nishino, K and Haesebrouck, Freddy and Ducatelle, Richard and Devreese, Bart and Van Immerseel, Filip},
  issn         = {0032-5791},
  journal      = {POULTRY SCIENCE},
  keywords     = {Salmonella Enteritidis,TolC,multidrug efflux pumps,egg white,ovotransferrin,ENTERICA SEROVAR ENTERITIDIS,MULTIDRUG EFFLUX SYSTEMS,REPRODUCTIVE-TRACT,CHICKEN,COLONIZATION,RESISTANCE,CONTAMINATION,GENES,HENS,BIOSYNTHESIS},
  language     = {eng},
  number       = {5},
  pages        = {2281--2289},
  title        = {The Salmonella Enteritidis TolC outer membrane channel is essential for egg white survival},
  url          = {http://dx.doi.org/10.3382/ps/pey584},
  volume       = {98},
  year         = {2019},
}

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