Colorimetric analysis is not sufficient to estimate bioavailable phosphorus in a hypersaline aquatic environment
- Author
- Chau Minh Khoi Chau Minh Khoi, Roel Merckx, Gilbert Van Stappen (UGent) , Huynh Thanh Toi and Nguyen Hoang Kim Nuong
- Organization
- Abstract
- Colorimetric analysis is commonly used to quantify P availability in aquatic environments. However, it may not be adequate to quantify the bioavailability of dissolved organic P compounds. This study aimed to investigate to what extent the colorimetrically measurable P fractions can be utilized by algae, with a particular emphasis on organic P compounds in a hypersaline aquatic environment. In this study, the growth of the marine alga Dunaliella tertiolecta Butcher was observed in axenic hypersaline cultures prepared with different organic P forms and related to the corresponding levels of colorimetrically measured P and total P. The malachite green method was used to colorimetrically analyze the different P forms and inductively coupled plasma-optical emission spectroscopy was used to quantify the total P. The results showed that only creatine P was colorimetrically detectable, of which 87% of its total concentration was measured. The growth of algae in the culture media with organic P compounds did not reflect the colorimetrically measurable organic P compounds. The results from this study imply that colorimetric analysis may not be sufficient to assess P availability to algae in hypersaline conditions where organic P components are the main source of P.
- Keywords
- ALKALINE-PHOSPHATASE ACTIVITY, DISSOLVED ORGANIC PHOSPHORUS, NUTRIENT-UPTAKE, COASTAL WATERS, GROWTH, NITROGEN, ALGAE, RATIO, SEA, Algal growth, Colorimetrically measurable P, P bioavailability
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8591985
- MLA
- Chau Minh Khoi, Chau Minh Khoi, et al. “Colorimetric Analysis Is Not Sufficient to Estimate Bioavailable Phosphorus in a Hypersaline Aquatic Environment.” HYDROBIOLOGIA, vol. 815, no. 1, 2018, pp. 177–86, doi:10.1007/s10750-018-3560-9.
- APA
- Chau Minh Khoi, C. M. K., Merckx, R., Van Stappen, G., Toi, H. T., & Nuong, N. H. K. (2018). Colorimetric analysis is not sufficient to estimate bioavailable phosphorus in a hypersaline aquatic environment. HYDROBIOLOGIA, 815(1), 177–186. https://doi.org/10.1007/s10750-018-3560-9
- Chicago author-date
- Chau Minh Khoi, Chau Minh Khoi, Roel Merckx, Gilbert Van Stappen, Huynh Thanh Toi, and Nguyen Hoang Kim Nuong. 2018. “Colorimetric Analysis Is Not Sufficient to Estimate Bioavailable Phosphorus in a Hypersaline Aquatic Environment.” HYDROBIOLOGIA 815 (1): 177–86. https://doi.org/10.1007/s10750-018-3560-9.
- Chicago author-date (all authors)
- Chau Minh Khoi, Chau Minh Khoi, Roel Merckx, Gilbert Van Stappen, Huynh Thanh Toi, and Nguyen Hoang Kim Nuong. 2018. “Colorimetric Analysis Is Not Sufficient to Estimate Bioavailable Phosphorus in a Hypersaline Aquatic Environment.” HYDROBIOLOGIA 815 (1): 177–186. doi:10.1007/s10750-018-3560-9.
- Vancouver
- 1.Chau Minh Khoi CMK, Merckx R, Van Stappen G, Toi HT, Nuong NHK. Colorimetric analysis is not sufficient to estimate bioavailable phosphorus in a hypersaline aquatic environment. HYDROBIOLOGIA. 2018;815(1):177–86.
- IEEE
- [1]C. M. K. Chau Minh Khoi, R. Merckx, G. Van Stappen, H. T. Toi, and N. H. K. Nuong, “Colorimetric analysis is not sufficient to estimate bioavailable phosphorus in a hypersaline aquatic environment,” HYDROBIOLOGIA, vol. 815, no. 1, pp. 177–186, 2018.
@article{8591985, abstract = {{Colorimetric analysis is commonly used to quantify P availability in aquatic environments. However, it may not be adequate to quantify the bioavailability of dissolved organic P compounds. This study aimed to investigate to what extent the colorimetrically measurable P fractions can be utilized by algae, with a particular emphasis on organic P compounds in a hypersaline aquatic environment. In this study, the growth of the marine alga Dunaliella tertiolecta Butcher was observed in axenic hypersaline cultures prepared with different organic P forms and related to the corresponding levels of colorimetrically measured P and total P. The malachite green method was used to colorimetrically analyze the different P forms and inductively coupled plasma-optical emission spectroscopy was used to quantify the total P. The results showed that only creatine P was colorimetrically detectable, of which 87% of its total concentration was measured. The growth of algae in the culture media with organic P compounds did not reflect the colorimetrically measurable organic P compounds. The results from this study imply that colorimetric analysis may not be sufficient to assess P availability to algae in hypersaline conditions where organic P components are the main source of P.}}, author = {{Chau Minh Khoi, Chau Minh Khoi and Merckx, Roel and Van Stappen, Gilbert and Toi, Huynh Thanh and Nuong, Nguyen Hoang Kim}}, issn = {{0018-8158}}, journal = {{HYDROBIOLOGIA}}, keywords = {{ALKALINE-PHOSPHATASE ACTIVITY,DISSOLVED ORGANIC PHOSPHORUS,NUTRIENT-UPTAKE,COASTAL WATERS,GROWTH,NITROGEN,ALGAE,RATIO,SEA,Algal growth,Colorimetrically measurable P,P bioavailability}}, language = {{eng}}, number = {{1}}, pages = {{177--186}}, title = {{Colorimetric analysis is not sufficient to estimate bioavailable phosphorus in a hypersaline aquatic environment}}, url = {{http://doi.org/10.1007/s10750-018-3560-9}}, volume = {{815}}, year = {{2018}}, }
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