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Colorimetric analysis is not sufficient to estimate bioavailable phosphorus in a hypersaline aquatic environment

(2018) HYDROBIOLOGIA. 815(1). p.177-186
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Abstract
Colorimetric analysis is commonly used to quantify P availability in aquatic environments. However, it may not be adequate to quantify the bioavailability of dissolved organic P compounds. This study aimed to investigate to what extent the colorimetrically measurable P fractions can be utilized by algae, with a particular emphasis on organic P compounds in a hypersaline aquatic environment. In this study, the growth of the marine alga Dunaliella tertiolecta Butcher was observed in axenic hypersaline cultures prepared with different organic P forms and related to the corresponding levels of colorimetrically measured P and total P. The malachite green method was used to colorimetrically analyze the different P forms and inductively coupled plasma-optical emission spectroscopy was used to quantify the total P. The results showed that only creatine P was colorimetrically detectable, of which 87% of its total concentration was measured. The growth of algae in the culture media with organic P compounds did not reflect the colorimetrically measurable organic P compounds. The results from this study imply that colorimetric analysis may not be sufficient to assess P availability to algae in hypersaline conditions where organic P components are the main source of P.
Keywords
ALKALINE-PHOSPHATASE ACTIVITY, DISSOLVED ORGANIC PHOSPHORUS, NUTRIENT-UPTAKE, COASTAL WATERS, GROWTH, NITROGEN, ALGAE, RATIO, SEA, Algal growth, Colorimetrically measurable P, P bioavailability

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Citation

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MLA
Chau Minh Khoi, Chau Minh Khoi et al. “Colorimetric Analysis Is Not Sufficient to Estimate Bioavailable Phosphorus in a Hypersaline Aquatic Environment.” HYDROBIOLOGIA 815.1 (2018): 177–186. Print.
APA
Chau Minh Khoi, C. M. K., Merckx, R., Van Stappen, G., Toi, H. T., & Nuong, N. H. K. (2018). Colorimetric analysis is not sufficient to estimate bioavailable phosphorus in a hypersaline aquatic environment. HYDROBIOLOGIA, 815(1), 177–186.
Chicago author-date
Chau Minh Khoi, Chau Minh Khoi, Roel Merckx, Gilbert Van Stappen, Huynh Thanh Toi, and Nguyen Hoang Kim Nuong. 2018. “Colorimetric Analysis Is Not Sufficient to Estimate Bioavailable Phosphorus in a Hypersaline Aquatic Environment.” Hydrobiologia 815 (1): 177–186.
Chicago author-date (all authors)
Chau Minh Khoi, Chau Minh Khoi, Roel Merckx, Gilbert Van Stappen, Huynh Thanh Toi, and Nguyen Hoang Kim Nuong. 2018. “Colorimetric Analysis Is Not Sufficient to Estimate Bioavailable Phosphorus in a Hypersaline Aquatic Environment.” Hydrobiologia 815 (1): 177–186.
Vancouver
1.
Chau Minh Khoi CMK, Merckx R, Van Stappen G, Toi HT, Nuong NHK. Colorimetric analysis is not sufficient to estimate bioavailable phosphorus in a hypersaline aquatic environment. HYDROBIOLOGIA. 2018;815(1):177–86.
IEEE
[1]
C. M. K. Chau Minh Khoi, R. Merckx, G. Van Stappen, H. T. Toi, and N. H. K. Nuong, “Colorimetric analysis is not sufficient to estimate bioavailable phosphorus in a hypersaline aquatic environment,” HYDROBIOLOGIA, vol. 815, no. 1, pp. 177–186, 2018.
@article{8591985,
  abstract     = {Colorimetric analysis is commonly used to quantify P availability in aquatic environments. However, it may not be adequate to quantify the bioavailability of dissolved organic P compounds. This study aimed to investigate to what extent the colorimetrically measurable P fractions can be utilized by algae, with a particular emphasis on organic P compounds in a hypersaline aquatic environment. In this study, the growth of the marine alga Dunaliella tertiolecta Butcher was observed in axenic hypersaline cultures prepared with different organic P forms and related to the corresponding levels of colorimetrically measured P and total P. The malachite green method was used to colorimetrically analyze the different P forms and inductively coupled plasma-optical emission spectroscopy was used to quantify the total P. The results showed that only creatine P was colorimetrically detectable, of which 87% of its total concentration was measured. The growth of algae in the culture media with organic P compounds did not reflect the colorimetrically measurable organic P compounds. The results from this study imply that colorimetric analysis may not be sufficient to assess P availability to algae in hypersaline conditions where organic P components are the main source of P.},
  author       = {Chau Minh Khoi, Chau Minh Khoi and Merckx, Roel and Van Stappen, Gilbert and Toi, Huynh Thanh and Nuong, Nguyen Hoang Kim},
  issn         = {0018-8158},
  journal      = {HYDROBIOLOGIA},
  keywords     = {ALKALINE-PHOSPHATASE ACTIVITY,DISSOLVED ORGANIC PHOSPHORUS,NUTRIENT-UPTAKE,COASTAL WATERS,GROWTH,NITROGEN,ALGAE,RATIO,SEA,Algal growth,Colorimetrically measurable P,P bioavailability},
  language     = {eng},
  number       = {1},
  pages        = {177--186},
  title        = {Colorimetric analysis is not sufficient to estimate bioavailable phosphorus in a hypersaline aquatic environment},
  url          = {http://dx.doi.org/10.1007/s10750-018-3560-9},
  volume       = {815},
  year         = {2018},
}

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