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Ionic stress enhances ER-PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis

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Abstract
The interorganelle communication mediated by membrane contact sites (MCSs) is an evolutionary hallmark of eukaryotic cells. MCS connections enable the nonvesicular exchange of information between organelles and allow them to coordinate responses to changing cellular environments. In plants, the importance of MCS components in the responses to environmental stress has been widely established, but the molecular mechanisms regulating interorganelle connectivity during stress still remain opaque. In this report, we use the model plant Arabidopsis thaliana to show that ionic stress increases endoplasmic reticulum (ER)-plasma membrane (PM) connectivity by promoting the cortical expansion of synaptotagmin 1 (SYT1)-enriched ER-PM contact sites (S-EPCSs). We define differential roles for the cortical cytoskeleton in the regulation of S-EPCS dynamics and ER-PM connectivity, and we identify the accumulation of phosphatidylinositol 4,5-bisphosphate [PI(4,5) P-2] at the PM as a molecular signal associated with the ER-PM connectivity changes. Our study highlights the functional conservation of EPCS components and PM phosphoinositides as modulators of ER-PM connectivity in eukaryotes, and uncovers unique aspects of the spatiotemporal regulation of ER-PM connectivity in plants.
Keywords
ER-PM contact sites, SYT1, cytoskeleton, ionic stress, phosphoinositides, ENDOPLASMIC-RETICULUM, PLASMA-MEMBRANE, LIPID TRAFFICKING, PHOSPHOLIPASE-C, SYNAPTOTAGMIN 1, PROTEINS, CALCIUM, BINDING, ENDOCYTOSIS, CELLS

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MLA
Lee, Eunkyoung et al. “Ionic Stress Enhances ER-PM Connectivity via Phosphoinositide-associated SYT1 Contact Site Expansion in Arabidopsis.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.4 (2019): 1420–1429. Print.
APA
Lee, E., Vanneste, S., Perez-Sancho, J., Benitez-Fuente, F., Strelau, M., Macho, A. P., Botella, M. A., et al. (2019). Ionic stress enhances ER-PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 116(4), 1420–1429.
Chicago author-date
Lee, Eunkyoung, Steffen Vanneste, Jessica Perez-Sancho, Francisco Benitez-Fuente, Matthew Strelau, Alberto P Macho, Miguel A Botella, Jiri Friml, and Abel Rosado. 2019. “Ionic Stress Enhances ER-PM Connectivity via Phosphoinositide-associated SYT1 Contact Site Expansion in Arabidopsis.” Proceedings of the National Academy of Sciences of the United States of America 116 (4): 1420–1429.
Chicago author-date (all authors)
Lee, Eunkyoung, Steffen Vanneste, Jessica Perez-Sancho, Francisco Benitez-Fuente, Matthew Strelau, Alberto P Macho, Miguel A Botella, Jiri Friml, and Abel Rosado. 2019. “Ionic Stress Enhances ER-PM Connectivity via Phosphoinositide-associated SYT1 Contact Site Expansion in Arabidopsis.” Proceedings of the National Academy of Sciences of the United States of America 116 (4): 1420–1429.
Vancouver
1.
Lee E, Vanneste S, Perez-Sancho J, Benitez-Fuente F, Strelau M, Macho AP, et al. Ionic stress enhances ER-PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2019;116(4):1420–9.
IEEE
[1]
E. Lee et al., “Ionic stress enhances ER-PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis,” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 116, no. 4, pp. 1420–1429, 2019.
@article{8591948,
  abstract     = {The interorganelle communication mediated by membrane contact sites (MCSs) is an evolutionary hallmark of eukaryotic cells. MCS connections enable the nonvesicular exchange of information between organelles and allow them to coordinate responses to changing cellular environments. In plants, the importance of MCS components in the responses to environmental stress has been widely established, but the molecular mechanisms regulating interorganelle connectivity during stress still remain opaque. In this report, we use the model plant Arabidopsis thaliana to show that ionic stress increases endoplasmic reticulum (ER)-plasma membrane (PM) connectivity by promoting the cortical expansion of synaptotagmin 1 (SYT1)-enriched ER-PM contact sites (S-EPCSs). We define differential roles for the cortical cytoskeleton in the regulation of S-EPCS dynamics and ER-PM connectivity, and we identify the accumulation of phosphatidylinositol 4,5-bisphosphate [PI(4,5) P-2] at the PM as a molecular signal associated with the ER-PM connectivity changes. Our study highlights the functional conservation of EPCS components and PM phosphoinositides as modulators of ER-PM connectivity in eukaryotes, and uncovers unique aspects of the spatiotemporal regulation of ER-PM connectivity in plants.},
  author       = {Lee, Eunkyoung and Vanneste, Steffen and Perez-Sancho, Jessica and Benitez-Fuente, Francisco and Strelau, Matthew and Macho, Alberto P and Botella, Miguel A and Friml, Jiri and Rosado, Abel},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keywords     = {ER-PM contact sites,SYT1,cytoskeleton,ionic stress,phosphoinositides,ENDOPLASMIC-RETICULUM,PLASMA-MEMBRANE,LIPID TRAFFICKING,PHOSPHOLIPASE-C,SYNAPTOTAGMIN 1,PROTEINS,CALCIUM,BINDING,ENDOCYTOSIS,CELLS},
  language     = {eng},
  number       = {4},
  pages        = {1420--1429},
  title        = {Ionic stress enhances ER-PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis},
  url          = {http://dx.doi.org/10.1073/pnas.1818099116},
  volume       = {116},
  year         = {2019},
}

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