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Effect of quorum quenching bacteria on growth, virulence factors and biofilm formation of Yersinia ruckeri invitro and an invivo evaluation of their probiotic effect in rainbow trout

(2018) JOURNAL OF FISH DISEASES. 41(9). p.1429-1438
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Abstract
Five N-acyl homoserine lactone-degrading bacteria (quorum quenching (QQ) strains) were selected to evaluate their impacts on growth, virulence factors and biofilm formation in Yersinia ruckeri invitro. No difference was observed among the growth pattern of Y.ruckeri in monoculture and coculture with the QQ strains. To investigate the regulation of virulence factors by quorum sensing in Y.ruckeri, cultures were supplemented with 3oxo-C8-HSL. The results indicated that swimming motility and biofilm formation are positively regulated by QS (p<0.05), whereas caseinase, phospholipase and haemolysin productions are not influenced by 3oxo-C8-HSL (p>0.05). The QQs were able to decrease swimming motility and biofilm formation in Y.ruckeri. QQ bacteria were supplemented to trout feed at 10(8)CFU/g (for 40days). Their probiotic effect was verified by Y.ruckeri challenge either by immersion or injection in trout. All strains could significantly increase fish survival with Bacillus thuringiensis and Citrobacter gillenii showing the highest and lowest relative percentage survival (RPS) values (respectively, 85% and 38%). Besides, there was no difference between the RPS values by either immersion or injection challenge expect for B.thuringiensis. The putative involvement of the QQ capacity in the protection against Yersinia is discussed.
Keywords
ACYLATED HOMOSERINE LACTONES, N-ACYLHOMOSERINE LACTONES, PSEUDOMONAS-AERUGINOSA, SIGNAL MOLECULES, N-(3-OXODODECANOYL)-L-HOMOSERINE LACTONE, AEROMONAS-HYDROPHILA, SALMO-GAIRDNERI, SENSING SYSTEM, IDENTIFICATION, EXPRESSION, biofilm, enteric redmouth disease, probiotic bacteria, Yersinia ruckeri

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Citation

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MLA
Delshad, Somayeh Torabi, Siyavash Soltanian, Hassan Sharifiyazdi, et al. “Effect of Quorum Quenching Bacteria on Growth, Virulence Factors and Biofilm Formation of Yersinia Ruckeri Invitro and an Invivo Evaluation of Their Probiotic Effect in Rainbow Trout.” JOURNAL OF FISH DISEASES 41.9 (2018): 1429–1438. Print.
APA
Delshad, S. T., Soltanian, S., Sharifiyazdi, H., & Bossier, P. (2018). Effect of quorum quenching bacteria on growth, virulence factors and biofilm formation of Yersinia ruckeri invitro and an invivo evaluation of their probiotic effect in rainbow trout. JOURNAL OF FISH DISEASES, 41(9), 1429–1438.
Chicago author-date
Delshad, Somayeh Torabi, Siyavash Soltanian, Hassan Sharifiyazdi, and Peter Bossier. 2018. “Effect of Quorum Quenching Bacteria on Growth, Virulence Factors and Biofilm Formation of Yersinia Ruckeri Invitro and an Invivo Evaluation of Their Probiotic Effect in Rainbow Trout.” Journal of Fish Diseases 41 (9): 1429–1438.
Chicago author-date (all authors)
Delshad, Somayeh Torabi, Siyavash Soltanian, Hassan Sharifiyazdi, and Peter Bossier. 2018. “Effect of Quorum Quenching Bacteria on Growth, Virulence Factors and Biofilm Formation of Yersinia Ruckeri Invitro and an Invivo Evaluation of Their Probiotic Effect in Rainbow Trout.” Journal of Fish Diseases 41 (9): 1429–1438.
Vancouver
1.
Delshad ST, Soltanian S, Sharifiyazdi H, Bossier P. Effect of quorum quenching bacteria on growth, virulence factors and biofilm formation of Yersinia ruckeri invitro and an invivo evaluation of their probiotic effect in rainbow trout. JOURNAL OF FISH DISEASES. 2018;41(9):1429–38.
IEEE
[1]
S. T. Delshad, S. Soltanian, H. Sharifiyazdi, and P. Bossier, “Effect of quorum quenching bacteria on growth, virulence factors and biofilm formation of Yersinia ruckeri invitro and an invivo evaluation of their probiotic effect in rainbow trout,” JOURNAL OF FISH DISEASES, vol. 41, no. 9, pp. 1429–1438, 2018.
@article{8591816,
  abstract     = {Five N-acyl homoserine lactone-degrading bacteria (quorum quenching (QQ) strains) were selected to evaluate their impacts on growth, virulence factors and biofilm formation in Yersinia ruckeri invitro. No difference was observed among the growth pattern of Y.ruckeri in monoculture and coculture with the QQ strains. To investigate the regulation of virulence factors by quorum sensing in Y.ruckeri, cultures were supplemented with 3oxo-C8-HSL. The results indicated that swimming motility and biofilm formation are positively regulated by QS (p<0.05), whereas caseinase, phospholipase and haemolysin productions are not influenced by 3oxo-C8-HSL (p>0.05). The QQs were able to decrease swimming motility and biofilm formation in Y.ruckeri. QQ bacteria were supplemented to trout feed at 10(8)CFU/g (for 40days). Their probiotic effect was verified by Y.ruckeri challenge either by immersion or injection in trout. All strains could significantly increase fish survival with Bacillus thuringiensis and Citrobacter gillenii showing the highest and lowest relative percentage survival (RPS) values (respectively, 85% and 38%). Besides, there was no difference between the RPS values by either immersion or injection challenge expect for B.thuringiensis. The putative involvement of the QQ capacity in the protection against Yersinia is discussed.},
  author       = {Delshad, Somayeh Torabi and Soltanian, Siyavash and Sharifiyazdi, Hassan and Bossier, Peter},
  issn         = {0140-7775},
  journal      = {JOURNAL OF FISH DISEASES},
  keywords     = {ACYLATED HOMOSERINE LACTONES,N-ACYLHOMOSERINE LACTONES,PSEUDOMONAS-AERUGINOSA,SIGNAL MOLECULES,N-(3-OXODODECANOYL)-L-HOMOSERINE LACTONE,AEROMONAS-HYDROPHILA,SALMO-GAIRDNERI,SENSING SYSTEM,IDENTIFICATION,EXPRESSION,biofilm,enteric redmouth disease,probiotic bacteria,Yersinia ruckeri},
  language     = {eng},
  number       = {9},
  pages        = {1429--1438},
  title        = {Effect of quorum quenching bacteria on growth, virulence factors and biofilm formation of Yersinia ruckeri invitro and an invivo evaluation of their probiotic effect in rainbow trout},
  url          = {http://dx.doi.org/10.1111/jfd.12840},
  volume       = {41},
  year         = {2018},
}

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