Advanced search
1 file | 804.71 KB Add to list

Recombinant ferritin-H induces immunosuppression in European sea bass larvae (Dicentrarchus labrax) rather than immunostimulation and protection against a Vibrio anguillarum infection

Author
Organization
Abstract
Vibrio anguillarum causes high mortality in European sea bass (Dicentrarchus labrax) larviculture. In this study, we evaluated if the recombinant sea bass ferritin-H could stimulate the innate immune system of gnotobiotic European sea bass larvae resulting in protection against a V. anguillarum challenge. We also evaluated the effect of a V. anguillarum infection on the transcription of immune-related genes in gnotobiotic European sea bass larvae. Recombinant sea bass ferritin-H was produced, encapsulated in calcium alginate microparticles and orally delivered to sea bass larvae at seven days after hatching. Our results showed V. anguillarum caused an acute infection, resulting in high mortality. The infection significantly upregulated the expression of tlr3, tlr5, casl, il1 beta, tnf alpha, mif, il10, cc1, cxcl8 at 18, 24 and 36 h post infection, but not of the chemokine receptor genes cxcr4 and ccr9. There was no protective effect of ferritin-H. Remarkably, ferritin-H caused significantly higher transcript levels for cxcr4 and ccr9. Sea bass ferritin-H was more likely involved in immune-suppression and results point in the direction of a negative regulation of CXCR4 resulting in inhibition of cell proliferation, differentiation and migration which is detrimental to innate immunity and might explain the non-protective effect of ferritin-H in fish larvae.
Keywords
Sea bass, Larvae, Gnotobiotic, Vibrio anguillarum, Ferritin, Innate immunity, HEAVY-CHAIN FERRITIN, IMMUNE-RESPONSE, IN-VIVO, CHALLENGE TEST, MESSENGER-RNA, FISH PATHOGEN, MODEL PROTEIN, EXPRESSION, SUBUNITS, CELLS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 804.71 KB

Citation

Please use this url to cite or link to this publication:

MLA
Yaacob, Eamy Nursaliza et al. “Recombinant ferritin-H Induces Immunosuppression in European Sea Bass Larvae (Dicentrarchus Labrax) Rather Than Immunostimulation and Protection Against a Vibrio Anguillarum Infection.” VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 204 (2018): 19–27. Print.
APA
Yaacob, E. N., De Geest, B., Goethals, J., Bajek, A., Dierckens, K., Bossier, P., & Vanrompay, D. (2018). Recombinant ferritin-H induces immunosuppression in European sea bass larvae (Dicentrarchus labrax) rather than immunostimulation and protection against a Vibrio anguillarum infection. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 204, 19–27.
Chicago author-date
Yaacob, Eamy Nursaliza, Bruno De Geest, Jens Goethals, Aline Bajek, Kristof Dierckens, Peter Bossier, and Daisy Vanrompay. 2018. “Recombinant ferritin-H Induces Immunosuppression in European Sea Bass Larvae (Dicentrarchus Labrax) Rather Than Immunostimulation and Protection Against a Vibrio Anguillarum Infection.” Veterinary Immunology and Immunopathology 204: 19–27.
Chicago author-date (all authors)
Yaacob, Eamy Nursaliza, Bruno De Geest, Jens Goethals, Aline Bajek, Kristof Dierckens, Peter Bossier, and Daisy Vanrompay. 2018. “Recombinant ferritin-H Induces Immunosuppression in European Sea Bass Larvae (Dicentrarchus Labrax) Rather Than Immunostimulation and Protection Against a Vibrio Anguillarum Infection.” Veterinary Immunology and Immunopathology 204: 19–27.
Vancouver
1.
Yaacob EN, De Geest B, Goethals J, Bajek A, Dierckens K, Bossier P, et al. Recombinant ferritin-H induces immunosuppression in European sea bass larvae (Dicentrarchus labrax) rather than immunostimulation and protection against a Vibrio anguillarum infection. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY. 2018;204:19–27.
IEEE
[1]
E. N. Yaacob et al., “Recombinant ferritin-H induces immunosuppression in European sea bass larvae (Dicentrarchus labrax) rather than immunostimulation and protection against a Vibrio anguillarum infection,” VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, vol. 204, pp. 19–27, 2018.
@article{8591552,
  abstract     = {Vibrio anguillarum causes high mortality in European sea bass (Dicentrarchus labrax) larviculture. In this study, we evaluated if the recombinant sea bass ferritin-H could stimulate the innate immune system of gnotobiotic European sea bass larvae resulting in protection against a V. anguillarum challenge. We also evaluated the effect of a V. anguillarum infection on the transcription of immune-related genes in gnotobiotic European sea bass larvae. Recombinant sea bass ferritin-H was produced, encapsulated in calcium alginate microparticles and orally delivered to sea bass larvae at seven days after hatching. Our results showed V. anguillarum caused an acute infection, resulting in high mortality. The infection significantly upregulated the expression of tlr3, tlr5, casl, il1 beta, tnf alpha, mif, il10, cc1, cxcl8 at 18, 24 and 36 h post infection, but not of the chemokine receptor genes cxcr4 and ccr9. There was no protective effect of ferritin-H. Remarkably, ferritin-H caused significantly higher transcript levels for cxcr4 and ccr9. Sea bass ferritin-H was more likely involved in immune-suppression and results point in the direction of a negative regulation of CXCR4 resulting in inhibition of cell proliferation, differentiation and migration which is detrimental to innate immunity and might explain the non-protective effect of ferritin-H in fish larvae.},
  author       = {Yaacob, Eamy Nursaliza and De Geest, Bruno and Goethals, Jens and Bajek, Aline and Dierckens, Kristof and Bossier, Peter and Vanrompay, Daisy},
  issn         = {0165-2427},
  journal      = {VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY},
  keywords     = {Sea bass,Larvae,Gnotobiotic,Vibrio anguillarum,Ferritin,Innate immunity,HEAVY-CHAIN FERRITIN,IMMUNE-RESPONSE,IN-VIVO,CHALLENGE TEST,MESSENGER-RNA,FISH PATHOGEN,MODEL PROTEIN,EXPRESSION,SUBUNITS,CELLS},
  language     = {eng},
  pages        = {19--27},
  title        = {Recombinant ferritin-H induces immunosuppression in European sea bass larvae (Dicentrarchus labrax) rather than immunostimulation and protection against a Vibrio anguillarum infection},
  url          = {http://dx.doi.org/10.1016/j.vetimm.2018.09.001},
  volume       = {204},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: