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The Meloidogyne graminicola effector Mg16820 is secreted in the apoplast and cytoplasm to suppress plant host defense responses

(2018) MOLECULAR PLANT PATHOLOGY. 19(11). p.2416-2430
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Abstract
On invasion of roots, plant-parasitic nematodes secrete effectors to manipulate the cellular regulation of the host to promote parasitism. The root-knot nematode Meloidogyne graminicola is one of the most damaging nematodes of rice. Here, we identified a novel effector of this nematode, named Mg16820, expressed in the nematode subventral glands. We localized the Mg16820 effector in the apoplast during the migration phase of the second-stage juvenile in rice roots. In addition, during early development of the feeding site, Mg16820 was localized in giant cells, where it accumulated in the cytoplasm and the nucleus. Using transient expression in Nicotiana benthamiana leaves, we demonstrated that Mg16820 directed to the apoplast was able to suppress flg22-induced reactive oxygen species production. In addition, expression of Mg16820 in the cytoplasm resulted in the suppression of the R2/Avr2- and Mi-1.2-induced hypersensitive response. A potential target protein of Mg16820 identified with the yeast two-hybrid system was the dehydration stress-inducible protein 1 (DIP1). Bimolecular fluorescence complementation resulted in a strong signal in the nucleus. DIP1 has been described as an abscisic acid (ABA)-responsive gene and ABA is involved in the biotic and abiotic stress response. Our results demonstrate that Mg16820 is able to act in two cellular compartments as an immune suppressor and targets a protein involved in the stress response, therefore indicating an important role for this effector in parasitism.
Keywords
IN-SITU HYBRIDIZATION, NEMATODE PARASITISM, DISEASE RESISTANCE, GIANT-CELLS, PROTEIN, GENES, RNA, TRANSCRIPTOME, PERCEPTION, IMMUNITY, effector-triggered immunity, Nicotiana benthamiana, Oryza sativa, PAMP-triggered immunity, parasitism, reactive oxygen species, root-knot, nematodes

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Citation

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MLA
Naalden, Diana et al. “The Meloidogyne Graminicola Effector Mg16820 Is Secreted in the Apoplast and Cytoplasm to Suppress Plant Host Defense Responses.” MOLECULAR PLANT PATHOLOGY 19.11 (2018): 2416–2430. Print.
APA
Naalden, D., Haegeman, A., de Almeida-Engler, J., Eshetu, F. B., Bauters, L., & Gheysen, G. (2018). The Meloidogyne graminicola effector Mg16820 is secreted in the apoplast and cytoplasm to suppress plant host defense responses. MOLECULAR PLANT PATHOLOGY, 19(11), 2416–2430.
Chicago author-date
Naalden, Diana, Annelies Haegeman, Janice de Almeida-Engler, Firehiwot Birhane Eshetu, Lander Bauters, and Godelieve Gheysen. 2018. “The Meloidogyne Graminicola Effector Mg16820 Is Secreted in the Apoplast and Cytoplasm to Suppress Plant Host Defense Responses.” Molecular Plant Pathology 19 (11): 2416–2430.
Chicago author-date (all authors)
Naalden, Diana, Annelies Haegeman, Janice de Almeida-Engler, Firehiwot Birhane Eshetu, Lander Bauters, and Godelieve Gheysen. 2018. “The Meloidogyne Graminicola Effector Mg16820 Is Secreted in the Apoplast and Cytoplasm to Suppress Plant Host Defense Responses.” Molecular Plant Pathology 19 (11): 2416–2430.
Vancouver
1.
Naalden D, Haegeman A, de Almeida-Engler J, Eshetu FB, Bauters L, Gheysen G. The Meloidogyne graminicola effector Mg16820 is secreted in the apoplast and cytoplasm to suppress plant host defense responses. MOLECULAR PLANT PATHOLOGY. 2018;19(11):2416–30.
IEEE
[1]
D. Naalden, A. Haegeman, J. de Almeida-Engler, F. B. Eshetu, L. Bauters, and G. Gheysen, “The Meloidogyne graminicola effector Mg16820 is secreted in the apoplast and cytoplasm to suppress plant host defense responses,” MOLECULAR PLANT PATHOLOGY, vol. 19, no. 11, pp. 2416–2430, 2018.
@article{8591085,
  abstract     = {On invasion of roots, plant-parasitic nematodes secrete effectors to manipulate the cellular regulation of the host to promote parasitism. The root-knot nematode Meloidogyne graminicola is one of the most damaging nematodes of rice. Here, we identified a novel effector of this nematode, named Mg16820, expressed in the nematode subventral glands. We localized the Mg16820 effector in the apoplast during the migration phase of the second-stage juvenile in rice roots. In addition, during early development of the feeding site, Mg16820 was localized in giant cells, where it accumulated in the cytoplasm and the nucleus. Using transient expression in Nicotiana benthamiana leaves, we demonstrated that Mg16820 directed to the apoplast was able to suppress flg22-induced reactive oxygen species production. In addition, expression of Mg16820 in the cytoplasm resulted in the suppression of the R2/Avr2- and Mi-1.2-induced hypersensitive response. A potential target protein of Mg16820 identified with the yeast two-hybrid system was the dehydration stress-inducible protein 1 (DIP1). Bimolecular fluorescence complementation resulted in a strong signal in the nucleus. DIP1 has been described as an abscisic acid (ABA)-responsive gene and ABA is involved in the biotic and abiotic stress response. Our results demonstrate that Mg16820 is able to act in two cellular compartments as an immune suppressor and targets a protein involved in the stress response, therefore indicating an important role for this effector in parasitism.},
  author       = {Naalden, Diana and Haegeman, Annelies and de Almeida-Engler, Janice and Eshetu, Firehiwot Birhane and Bauters, Lander and Gheysen, Godelieve},
  issn         = {1464-6722},
  journal      = {MOLECULAR PLANT PATHOLOGY},
  keywords     = {IN-SITU HYBRIDIZATION,NEMATODE PARASITISM,DISEASE RESISTANCE,GIANT-CELLS,PROTEIN,GENES,RNA,TRANSCRIPTOME,PERCEPTION,IMMUNITY,effector-triggered immunity,Nicotiana benthamiana,Oryza sativa,PAMP-triggered immunity,parasitism,reactive oxygen species,root-knot,nematodes},
  language     = {eng},
  number       = {11},
  pages        = {2416--2430},
  title        = {The Meloidogyne graminicola effector Mg16820 is secreted in the apoplast and cytoplasm to suppress plant host defense responses},
  url          = {http://dx.doi.org/10.1111/mpp.12719},
  volume       = {19},
  year         = {2018},
}

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