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Quantification of a glucocorticoid profile in non-pooled samples is pivotal in stress research across vertebrates

Johan Aerts (UGent)
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Abstract
Vertebrates are faced continuously with a variety of potential stressful stimuli and react by a highly conserved endocrine stress response. An immediate catecholamine mediated response increases plasma glucose levels in order to prepare the organism for the "fight or flight" reaction. In addition, in a matter of minutes after this (nor) adrenaline release, glucocorticoids, in particular cortisol or corticosterone depending on the species, are released through activation of the hypothalamic-pituitary-interrenal (HPI) axis in fish or hypothalamic-pituitary-adrenal (HPA) axis in other vertebrates. These plasma glucocorticoids are well documented and widely used as biomarker for stress across vertebrates. In order to study the role of glucocorticoids in acute and chronic stress and gain in-depth insight in the stress axis (re)activity across vertebrates, it is pivotal to pin-point the involved molecules, to understand the mechanisms of how the latter are synthesized, regulated and excreted, and to grasp their actions on a plethora of biological processes. Furthermore, in-depth knowledge on the characteristics of the tissues as well as on the analytical methodologies available for glucocorticoid quantification is needed. This manuscript is to be situated in the multi-disciplinary research topic of glucocorticoid action across vertebrates which is linked to a wide range of research domains including but not limited to biochemistry, ecology, endocrinology, ethology, histology, immunology, morphology, physiology, and toxicology, and provides a solid base for all interested in stress, in particular glucocorticoid, related research. In this framework, internationally validated confirmation methods for quantification of a glucocorticoid profile comprising: (i) the dominant hormone; (ii) its direct precursors; (iii) its endogenously present phase I metabolites; and (iv) the most abundant more polar excreted exogenous phase I metabolites in non-pooled samples are pivotal.
Keywords
vertebrate, stress, HPI, HPA, glucocorticoid, profile, PITUITARY-ADRENAL AXIS, CORTICOSTEROID-BINDING GLOBULIN, MINERALOCORTICOID RECEPTOR, MASS-SPECTROMETRY, CORTISOL RESPONSES, STEROID-HORMONES, HPA AXIS, MECHANISMS, EXPRESSION, METABOLITES

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Citation

Please use this url to cite or link to this publication:

Chicago
Aerts, Johan. 2018. “Quantification of a Glucocorticoid Profile in Non-pooled Samples Is Pivotal in Stress Research Across Vertebrates.” Frontiers in Endocrinology 9.
APA
Aerts, J. (2018). Quantification of a glucocorticoid profile in non-pooled samples is pivotal in stress research across vertebrates. FRONTIERS IN ENDOCRINOLOGY, 9.
Vancouver
1.
Aerts J. Quantification of a glucocorticoid profile in non-pooled samples is pivotal in stress research across vertebrates. FRONTIERS IN ENDOCRINOLOGY. 2018;9.
MLA
Aerts, Johan. “Quantification of a Glucocorticoid Profile in Non-pooled Samples Is Pivotal in Stress Research Across Vertebrates.” FRONTIERS IN ENDOCRINOLOGY 9 (2018): n. pag. Print.
@article{8589844,
  abstract     = {Vertebrates are faced continuously with a variety of potential stressful stimuli and react by a highly conserved endocrine stress response. An immediate catecholamine mediated response increases plasma glucose levels in order to prepare the organism for the {\textacutedbl}fight or flight{\textacutedbl} reaction. In addition, in a matter of minutes after this (nor) adrenaline release, glucocorticoids, in particular cortisol or corticosterone depending on the species, are released through activation of the hypothalamic-pituitary-interrenal (HPI) axis in fish or hypothalamic-pituitary-adrenal (HPA) axis in other vertebrates. These plasma glucocorticoids are well documented and widely used as biomarker for stress across vertebrates. In order to study the role of glucocorticoids in acute and chronic stress and gain in-depth insight in the stress axis (re)activity across vertebrates, it is pivotal to pin-point the involved molecules, to understand the mechanisms of how the latter are synthesized, regulated and excreted, and to grasp their actions on a plethora of biological processes. Furthermore, in-depth knowledge on the characteristics of the tissues as well as on the analytical methodologies available for glucocorticoid quantification is needed. This manuscript is to be situated in the multi-disciplinary research topic of glucocorticoid action across vertebrates which is linked to a wide range of research domains including but not limited to biochemistry, ecology, endocrinology, ethology, histology, immunology, morphology, physiology, and toxicology, and provides a solid base for all interested in stress, in particular glucocorticoid, related research. In this framework, internationally validated confirmation methods for quantification of a glucocorticoid profile comprising: (i) the dominant hormone; (ii) its direct precursors; (iii) its endogenously present phase I metabolites; and (iv) the most abundant more polar excreted exogenous phase I metabolites in non-pooled samples are pivotal.},
  articleno    = {635},
  author       = {Aerts, Johan},
  issn         = {1664-2392},
  journal      = {FRONTIERS IN ENDOCRINOLOGY},
  language     = {eng},
  pages        = {9},
  title        = {Quantification of a glucocorticoid profile in non-pooled samples is pivotal in stress research across vertebrates},
  url          = {http://dx.doi.org/10.3389/fendo.2018.00635},
  volume       = {9},
  year         = {2018},
}

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