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Plasma functionalization of polycaprolactone nanofibers changes protein interactions with cells, resulting in increased cell viability

Mahtab Asadian (UGent) , Maarten Dhaenens (UGent) , Yuliia Onyshchenko (UGent) , Stijn De Waele (UGent) , Heidi Declercq (UGent) , Pieter Cools (UGent) , Bart Devreese (UGent) , Dieter Deforce (UGent) , Rino Morent (UGent) and Nathalie De Geyter (UGent)
(2018) ACS APPLIED MATERIALS & INTERFACES. 10(49). p.41962-41977
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Abstract
The surface properties of electrospun scaffolds can greatly influence protein adsorption and, thus, strongly dictate cell-material interactions. In this study, we aim to investigate possible correlations between the surface properties of argon, nitrogen, and ammonia and helium plasma-functionalized polycaprolactone (PCL) nanofibers (NFs) and their cellular interactions by examining the protein corona patterns of the plasma-treated NFs as well as the cell membrane proteins involved in cell proliferation. As a result of the performed plasma treatments, PCL NFs morphology was preserved, while wettability was improved profoundly after all treatments because of the incorporation of polar surface groups. Depending on the discharge gas, different types of groups are incorporated, which influenced the resultant cell-material interactions. Argon plasma-functionalized PCL NFs, only enriched by oxygen-containing functional groups, were found to show the best cell-material interactions, followed by N-2 and He/NH3 plasma-treated samples. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and liquid chromatography-mass spectrometry clearly indicated an increased protein retention compared with non-treated PCL NFs. The nine proteins retained best on plasma-treated NF are important mediators of extracellular matrix interaction, illustrating the importance thereof for cell proliferation and the viability of cells. Finally, 92 proteins that can be used to differentiate how the different plasma treatments are clustered and subjected to a gene ontology study, illustrating the importance of keratinization and extracellular matrix organization.
Keywords
nanofiber, plasma modification, protein corona, tissue regeneration, surface analysis, DBD plasma modification, DIELECTRIC BARRIER DISCHARGE, SURFACE MODIFICATION, PRESSURE PLASMA, ADSORPTION, SCAFFOLDS, ADHESION, POLYETHYLENE, NITROGEN, ALLYLAMINE, INTERFACE

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Citation

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MLA
Asadian, Mahtab, et al. “Plasma Functionalization of Polycaprolactone Nanofibers Changes Protein Interactions with Cells, Resulting in Increased Cell Viability.” ACS APPLIED MATERIALS & INTERFACES, vol. 10, no. 49, 2018, pp. 41962–77, doi:10.1021/acsami.8b14995.
APA
Asadian, M., Dhaenens, M., Onyshchenko, Y., De Waele, S., Declercq, H., Cools, P., … De Geyter, N. (2018). Plasma functionalization of polycaprolactone nanofibers changes protein interactions with cells, resulting in increased cell viability. ACS APPLIED MATERIALS & INTERFACES, 10(49), 41962–41977. https://doi.org/10.1021/acsami.8b14995
Chicago author-date
Asadian, Mahtab, Maarten Dhaenens, Yuliia Onyshchenko, Stijn De Waele, Heidi Declercq, Pieter Cools, Bart Devreese, Dieter Deforce, Rino Morent, and Nathalie De Geyter. 2018. “Plasma Functionalization of Polycaprolactone Nanofibers Changes Protein Interactions with Cells, Resulting in Increased Cell Viability.” ACS APPLIED MATERIALS & INTERFACES 10 (49): 41962–77. https://doi.org/10.1021/acsami.8b14995.
Chicago author-date (all authors)
Asadian, Mahtab, Maarten Dhaenens, Yuliia Onyshchenko, Stijn De Waele, Heidi Declercq, Pieter Cools, Bart Devreese, Dieter Deforce, Rino Morent, and Nathalie De Geyter. 2018. “Plasma Functionalization of Polycaprolactone Nanofibers Changes Protein Interactions with Cells, Resulting in Increased Cell Viability.” ACS APPLIED MATERIALS & INTERFACES 10 (49): 41962–41977. doi:10.1021/acsami.8b14995.
Vancouver
1.
Asadian M, Dhaenens M, Onyshchenko Y, De Waele S, Declercq H, Cools P, et al. Plasma functionalization of polycaprolactone nanofibers changes protein interactions with cells, resulting in increased cell viability. ACS APPLIED MATERIALS & INTERFACES. 2018;10(49):41962–77.
IEEE
[1]
M. Asadian et al., “Plasma functionalization of polycaprolactone nanofibers changes protein interactions with cells, resulting in increased cell viability,” ACS APPLIED MATERIALS & INTERFACES, vol. 10, no. 49, pp. 41962–41977, 2018.
@article{8589565,
  abstract     = {{The surface properties of electrospun scaffolds can greatly influence protein adsorption and, thus, strongly dictate cell-material interactions. In this study, we aim to investigate possible correlations between the surface properties of argon, nitrogen, and ammonia and helium plasma-functionalized polycaprolactone (PCL) nanofibers (NFs) and their cellular interactions by examining the protein corona patterns of the plasma-treated NFs as well as the cell membrane proteins involved in cell proliferation. As a result of the performed plasma treatments, PCL NFs morphology was preserved, while wettability was improved profoundly after all treatments because of the incorporation of polar surface groups. Depending on the discharge gas, different types of groups are incorporated, which influenced the resultant cell-material interactions. Argon plasma-functionalized PCL NFs, only enriched by oxygen-containing functional groups, were found to show the best cell-material interactions, followed by N-2 and He/NH3 plasma-treated samples. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and liquid chromatography-mass spectrometry clearly indicated an increased protein retention compared with non-treated PCL NFs. The nine proteins retained best on plasma-treated NF are important mediators of extracellular matrix interaction, illustrating the importance thereof for cell proliferation and the viability of cells. Finally, 92 proteins that can be used to differentiate how the different plasma treatments are clustered and subjected to a gene ontology study, illustrating the importance of keratinization and extracellular matrix organization.}},
  author       = {{Asadian, Mahtab and Dhaenens, Maarten and Onyshchenko, Yuliia and De Waele, Stijn and Declercq, Heidi and Cools, Pieter and Devreese, Bart and Deforce, Dieter and Morent, Rino and De Geyter, Nathalie}},
  issn         = {{1944-8244}},
  journal      = {{ACS APPLIED MATERIALS & INTERFACES}},
  keywords     = {{nanofiber,plasma modification,protein corona,tissue regeneration,surface analysis,DBD plasma modification,DIELECTRIC BARRIER DISCHARGE,SURFACE MODIFICATION,PRESSURE PLASMA,ADSORPTION,SCAFFOLDS,ADHESION,POLYETHYLENE,NITROGEN,ALLYLAMINE,INTERFACE}},
  language     = {{eng}},
  number       = {{49}},
  pages        = {{41962--41977}},
  title        = {{Plasma functionalization of polycaprolactone nanofibers changes protein interactions with cells, resulting in increased cell viability}},
  url          = {{http://dx.doi.org/10.1021/acsami.8b14995}},
  volume       = {{10}},
  year         = {{2018}},
}
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