Advanced search
1 file | 4.36 MB

Downregulation of MicroRNA eca-mir-128 in seminal exosomes and enhanced expression of CXCL16 in the stallion reproductive tract are associated with long-term persistence of equine arteritis virus

Author
Organization
Abstract
Equine arteritis virus (EAV) can establish long-term persistent infection in the reproductive tract of stallions and is shed in the semen. Previous studies showed that long-term persistence is associated with a specific allele of the CXCL16 gene (CXCL16S) and that persistent infection is maintained despite the presence of a local inflammatory and humoral and mucosal antibody responses. In this study, we demonstrated that equine seminal exosomes (SEs) are enriched in a small subset of microRNAs (miRNAs). Most importantly, we demonstrated that long-term EAV persistence is associated with the downregulation of an SE-associated miRNA (eca-mir-128) and with an enhanced expression of CXCL16 in the reproductive tract, a putative target of eca-mir-128. The findings presented here suggest that SE eca-mir-128 is implicated in the regulation of the CXCL16/CXCR6 axis in the reproductive tract of persistently infected stallions, a chemokine axis strongly implicated in EAV persistence. This is a novel finding and warrants further investigation to identify its specific mechanism in modulating the CXCL16/CXCR6 axis in the reproductive tract of the EAV long-term carrier stallion. IMPORTANCE: Equine arteritis virus (EAV) has the ability to establish long-term persistent infection in the stallion reproductive tract and to be shed in semen, which jeopardizes its worldwide control. Currently, the molecular mechanisms of viral persistence are being unraveled, and these are essential for the development of effective therapeutics to eliminate persistent infection. Recently, it has been determined that long-term persistence is associated with a specific allele of the CXCL16 gene (CXCL16S) and is maintained despite induction of local inflammatory, humoral, and mucosal antibody responses. This study demonstrated that long-term persistence is associated with the downregulation of seminal exosome miRNA eca-mir-128 and enhanced expression of its putative target, CXCL16, in the reproductive tract. For the first time, this study suggests complex interactions between eca-mir-128 and cellular elements at the site of EAV persistence and implicates this miRNA in the regulation of the CXCL16/CXCR6 axis in the reproductive tract during long-term persistence.
Keywords
equine arteritis virus, equine viral arteritis, EAV, EVA, seminal exosomes, miRNA, eca-mir-128, CXCL16, persistent infection, reproductive tract, EXTRACELLULAR ORGANELLES PROSTASOMES, INFECTIOUS CDNA-CLONE, DEEP SEQUENCING DATA, T-CELL-ACTIVATION, LARGE GENE LISTS, HUMAN SEMEN, VIRAL ARTERITIS, PCR DATA, TARGET PREDICTION, IMMUNE-RESPONSE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 4.36 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Carossino, Mariano, Pouya Dini, Theodore S Kalbfleisch, Alan T Loynachan, Igor F Canisso, Kathleen M Shuck, Peter J Timoney, R Frank Cook, and Udeni BR Balasuriya. 2018. “Downregulation of MicroRNA Eca-mir-128 in Seminal Exosomes and Enhanced Expression of CXCL16 in the Stallion Reproductive Tract Are Associated with Long-term Persistence of Equine Arteritis Virus.” Ed. Tom Gallagher. Journal of Virology 92 (9).
APA
Carossino, M., Dini, P., Kalbfleisch, T. S., Loynachan, A. T., Canisso, I. F., Shuck, K. M., Timoney, P. J., et al. (2018). Downregulation of MicroRNA eca-mir-128 in seminal exosomes and enhanced expression of CXCL16 in the stallion reproductive tract are associated with long-term persistence of equine arteritis virus. (T. Gallagher, Ed.)JOURNAL OF VIROLOGY, 92(9).
Vancouver
1.
Carossino M, Dini P, Kalbfleisch TS, Loynachan AT, Canisso IF, Shuck KM, et al. Downregulation of MicroRNA eca-mir-128 in seminal exosomes and enhanced expression of CXCL16 in the stallion reproductive tract are associated with long-term persistence of equine arteritis virus. Gallagher T, editor. JOURNAL OF VIROLOGY. 2018;92(9).
MLA
Carossino, Mariano et al. “Downregulation of MicroRNA Eca-mir-128 in Seminal Exosomes and Enhanced Expression of CXCL16 in the Stallion Reproductive Tract Are Associated with Long-term Persistence of Equine Arteritis Virus.” Ed. Tom Gallagher. JOURNAL OF VIROLOGY 92.9 (2018): n. pag. Print.
@article{8587779,
  abstract     = {Equine arteritis virus (EAV) can establish long-term persistent infection in the reproductive tract of stallions and is shed in the semen. Previous studies showed that long-term persistence is associated with a specific allele of the CXCL16 gene (CXCL16S) and that persistent infection is maintained despite the presence of a local inflammatory and humoral and mucosal antibody responses. In this study, we demonstrated that equine seminal exosomes (SEs) are enriched in a small subset of microRNAs (miRNAs). Most importantly, we demonstrated that long-term EAV persistence is associated with the downregulation of an SE-associated miRNA (eca-mir-128) and with an enhanced expression of CXCL16 in the reproductive tract, a putative target of eca-mir-128. The findings presented here suggest that SE eca-mir-128 is implicated in the regulation of the CXCL16/CXCR6 axis in the reproductive tract of persistently infected stallions, a chemokine axis strongly implicated in EAV persistence. This is a novel finding and warrants further investigation to identify its specific mechanism in modulating the CXCL16/CXCR6 axis in the reproductive tract of the EAV long-term carrier stallion. 
IMPORTANCE: Equine arteritis virus (EAV) has the ability to establish long-term persistent infection in the stallion reproductive tract and to be shed in semen, which jeopardizes its worldwide control. Currently, the molecular mechanisms of viral persistence are being unraveled, and these are essential for the development of effective therapeutics to eliminate persistent infection. Recently, it has been determined that long-term persistence is associated with a specific allele of the CXCL16 gene (CXCL16S) and is maintained despite induction of local inflammatory, humoral, and mucosal antibody responses. This study demonstrated that long-term persistence is associated with the downregulation of seminal exosome miRNA eca-mir-128 and enhanced expression of its putative target, CXCL16, in the reproductive tract. For the first time, this study suggests complex interactions between eca-mir-128 and cellular elements at the site of EAV persistence and implicates this miRNA in the regulation of the CXCL16/CXCR6 axis in the reproductive tract during long-term persistence.},
  articleno    = {e00015-18},
  author       = {Carossino, Mariano and Dini, Pouya and Kalbfleisch, Theodore S and Loynachan, Alan T and Canisso, Igor F and Shuck, Kathleen M and Timoney, Peter J and Cook, R Frank and Balasuriya, Udeni BR},
  editor       = {Gallagher, Tom},
  issn         = {0022-538X},
  journal      = {JOURNAL OF VIROLOGY},
  keywords     = {equine arteritis virus,equine viral arteritis,EAV,EVA,seminal exosomes,miRNA,eca-mir-128,CXCL16,persistent infection,reproductive tract,EXTRACELLULAR ORGANELLES PROSTASOMES,INFECTIOUS CDNA-CLONE,DEEP SEQUENCING DATA,T-CELL-ACTIVATION,LARGE GENE LISTS,HUMAN SEMEN,VIRAL ARTERITIS,PCR DATA,TARGET PREDICTION,IMMUNE-RESPONSE},
  language     = {eng},
  number       = {9},
  pages        = {23},
  title        = {Downregulation of MicroRNA eca-mir-128 in seminal exosomes and enhanced expression of CXCL16 in the stallion reproductive tract are associated with long-term persistence of equine arteritis virus},
  url          = {http://dx.doi.org/10.1128/jvi.00015-18},
  volume       = {92},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: