Advanced search
2 files | 3.77 MB Add to list

Chimeric LysR-type transcriptional biosensors for customizing ligand specificity profiles toward flavonoids

Brecht De Paepe (UGent) , Jo Maertens (UGent) , Bartel Vanholme (UGent) and Marjan De Mey (UGent)
(2019) ACS SYNTHETIC BIOLOGY. 8(2). p.318-331
Author
Organization
Abstract
Transcriptional biosensors enable key applications in both metabolic engineering and synthetic biology. Due to nature's immense variety of metabolites, these applications require biosensors with a ligand specificity profile customized to the researcher's needs. In this work, chimeric biosensors were created by introducing parts of a donor regulatory circuit from Sinorhizobium meliloti, delivering the desired luteolin-specific response, into a nonspecific biosensor chassis from Herbaspirillum seropedicae. Two strategies were evaluated for the development of chimeric LysR-type biosensors with customized ligand specificity profiles toward three closely related flavonoids, naringenin, apigenin, and luteolin. In the first strategy, chimeric promoter regions were constructed at the biosensor effector module, while in the second strategy, chimeric transcription factors were created at the biosensor detector module. Via both strategies, the biosensor repertoire was expanded with luteolin-specific chimeric biosensors demonstrating a variety of response curves and ligand specificity profiles. Starting from the nonspecific biosensor chassis, a shift from 27.5% to 95.3% luteolin specificity was achieved with the created chimeric biosensors. Both strategies provide a compelling, faster, and more accessible route for the customization of biosensor ligand specificity, compared to de novo design and construction of each biosensor circuit for every desired ligand specificity.
Keywords
transcriptional biosensors, ligand specificity engineering, flavonoids, chimeric genetic circuits, Escherichia coli, NARINGENIN DEGRADATION, ESCHERICHIA-COLI, NODULATION GENES, TET REPRESSOR, LA-CARTE, RHIZOBIUM, NODD, LUTEOLIN, BINDING, DNA

Downloads

  • acssynbio.8b00326.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 963.19 KB
  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.80 MB

Citation

Please use this url to cite or link to this publication:

MLA
De Paepe, Brecht, et al. “Chimeric LysR-Type Transcriptional Biosensors for Customizing Ligand Specificity Profiles toward Flavonoids.” ACS SYNTHETIC BIOLOGY, vol. 8, no. 2, 2019, pp. 318–31.
APA
De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2019). Chimeric LysR-type transcriptional biosensors for customizing ligand specificity profiles toward flavonoids. ACS SYNTHETIC BIOLOGY, 8(2), 318–331.
Chicago author-date
De Paepe, Brecht, Jo Maertens, Bartel Vanholme, and Marjan De Mey. 2019. “Chimeric LysR-Type Transcriptional Biosensors for Customizing Ligand Specificity Profiles toward Flavonoids.” ACS SYNTHETIC BIOLOGY 8 (2): 318–31.
Chicago author-date (all authors)
De Paepe, Brecht, Jo Maertens, Bartel Vanholme, and Marjan De Mey. 2019. “Chimeric LysR-Type Transcriptional Biosensors for Customizing Ligand Specificity Profiles toward Flavonoids.” ACS SYNTHETIC BIOLOGY 8 (2): 318–331.
Vancouver
1.
De Paepe B, Maertens J, Vanholme B, De Mey M. Chimeric LysR-type transcriptional biosensors for customizing ligand specificity profiles toward flavonoids. ACS SYNTHETIC BIOLOGY. 2019;8(2):318–31.
IEEE
[1]
B. De Paepe, J. Maertens, B. Vanholme, and M. De Mey, “Chimeric LysR-type transcriptional biosensors for customizing ligand specificity profiles toward flavonoids,” ACS SYNTHETIC BIOLOGY, vol. 8, no. 2, pp. 318–331, 2019.
@article{8587477,
  abstract     = {Transcriptional biosensors enable key applications in both metabolic engineering and synthetic biology. Due to nature's immense variety of metabolites, these applications require biosensors with a ligand specificity profile customized to the researcher's needs. In this work, chimeric biosensors were created by introducing parts of a donor regulatory circuit from Sinorhizobium meliloti, delivering the desired luteolin-specific response, into a nonspecific biosensor chassis from Herbaspirillum seropedicae. Two strategies were evaluated for the development of chimeric LysR-type biosensors with customized ligand specificity profiles toward three closely related flavonoids, naringenin, apigenin, and luteolin. In the first strategy, chimeric promoter regions were constructed at the biosensor effector module, while in the second strategy, chimeric transcription factors were created at the biosensor detector module. Via both strategies, the biosensor repertoire was expanded with luteolin-specific chimeric biosensors demonstrating a variety of response curves and ligand specificity profiles. Starting from the nonspecific biosensor chassis, a shift from 27.5% to 95.3% luteolin specificity was achieved with the created chimeric biosensors. Both strategies provide a compelling, faster, and more accessible route for the customization of biosensor ligand specificity, compared to de novo design and construction of each biosensor circuit for every desired ligand specificity.},
  author       = {De Paepe, Brecht and Maertens, Jo and Vanholme, Bartel and De Mey, Marjan},
  issn         = {2161-5063},
  journal      = {ACS SYNTHETIC BIOLOGY},
  keywords     = {transcriptional biosensors,ligand specificity engineering,flavonoids,chimeric genetic circuits,Escherichia coli,NARINGENIN DEGRADATION,ESCHERICHIA-COLI,NODULATION GENES,TET REPRESSOR,LA-CARTE,RHIZOBIUM,NODD,LUTEOLIN,BINDING,DNA},
  language     = {eng},
  number       = {2},
  pages        = {318--331},
  title        = {Chimeric LysR-type transcriptional biosensors for customizing ligand specificity profiles toward flavonoids},
  url          = {http://dx.doi.org/10.1021/acssynbio.8b00326},
  volume       = {8},
  year         = {2019},
}

Altmetric
View in Altmetric
Web of Science
Times cited: