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Legionella pneumophila modulates mitochondrial dynamics to trigger metabolic repurposing of infected macrophages

(2017) CELL HOST & MICROBE. 22(3). p.302-316
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Abstract
The intracellular bacteria Legionella pneumophila encodes a type IV secretion system (T4SS) that injects effector proteins into macrophages in order to establish and replicate within the Legionella-containing vacuole (LCV). Once generated, the LCV interacts with mitochondria through unclear mechanisms. We show that Legionella uses both T4SS-independent and T4SS-dependent mechanisms to respectively interact with mitochondria and induce mitochondrial fragmentation that ultimately alters mitochondrial metabolism. The T4SS effector MitF, a Ran GTPase activator, is required for fission of the mitochondrial network. These effects of MitF occur through accumulation of mitochondrial DNM1L, a GTPase critical for fission. Furthermore mitochondrial respiration is abruptly halted in a T4SS-dependent manner, while T4SS-independent upregulation of cellular glycolysis remains elevated. Collectively, these alterations in mitochondrial dynamics promote a Warburg-like phenotype in macrophages that favors bacterial replication. Hence the rewiring of cellular bioenergetics to create a replication permissive niche in host cells is a virulence strategy of L. pneumophila.
Keywords
HOST-CELL DEATH, INTRACELLULAR MULTIPLICATION, BACTERICIDAL ACTIVITY, FISSION, MEMBRANE, ACTIN, AUTOPHAGY, PROTEOME, FUSION, QUANTIFICATION

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Chicago
Escoll, Pedro, Ok-Ryul Song, Flávia Viana, Bernhard Steiner, Thibault Lagache, Jean-Christophe Olivo-Marin, Francis Impens, Priscille Brodin, Hubert Hilbi, and Carmen Buchrieser. 2017. “Legionella Pneumophila Modulates Mitochondrial Dynamics to Trigger Metabolic Repurposing of Infected Macrophages.” Cell Host & Microbe 22 (3): 302–316.
APA
Escoll, P., Song, O.-R., Viana, F., Steiner, B., Lagache, T., Olivo-Marin, J.-C., Impens, F., et al. (2017). Legionella pneumophila modulates mitochondrial dynamics to trigger metabolic repurposing of infected macrophages. CELL HOST & MICROBE, 22(3), 302–316.
Vancouver
1.
Escoll P, Song O-R, Viana F, Steiner B, Lagache T, Olivo-Marin J-C, et al. Legionella pneumophila modulates mitochondrial dynamics to trigger metabolic repurposing of infected macrophages. CELL HOST & MICROBE. 2017;22(3):302–16.
MLA
Escoll, Pedro et al. “Legionella Pneumophila Modulates Mitochondrial Dynamics to Trigger Metabolic Repurposing of Infected Macrophages.” CELL HOST & MICROBE 22.3 (2017): 302–316. Print.
@article{8587468,
  abstract     = {The intracellular bacteria Legionella pneumophila encodes a type IV secretion system (T4SS) that injects effector proteins into macrophages in order to establish and replicate within the Legionella-containing vacuole (LCV). Once generated, the LCV interacts with mitochondria through unclear mechanisms. We show that Legionella uses both T4SS-independent and T4SS-dependent mechanisms to respectively interact with mitochondria and induce mitochondrial fragmentation that ultimately alters mitochondrial metabolism. The T4SS effector MitF, a Ran GTPase activator, is required for fission of the mitochondrial network. These effects of MitF occur through accumulation of mitochondrial DNM1L, a GTPase critical for fission. Furthermore mitochondrial respiration is abruptly halted in a T4SS-dependent manner, while T4SS-independent upregulation of cellular glycolysis remains elevated. Collectively, these alterations in mitochondrial dynamics promote a Warburg-like phenotype in macrophages that favors bacterial replication. Hence the rewiring of cellular bioenergetics to create a replication permissive niche in host cells is a virulence strategy of L. pneumophila.},
  author       = {Escoll, Pedro and Song, Ok-Ryul and Viana, Fl{\'a}via and Steiner, Bernhard and Lagache, Thibault and Olivo-Marin, Jean-Christophe and Impens, Francis and Brodin, Priscille and Hilbi, Hubert and Buchrieser, Carmen},
  issn         = {1931-3128},
  journal      = {CELL HOST \& MICROBE},
  language     = {eng},
  number       = {3},
  pages        = {302--316},
  title        = {Legionella pneumophila modulates mitochondrial dynamics to trigger metabolic repurposing of infected macrophages},
  url          = {http://dx.doi.org/10.1016/j.chom.2017.07.020},
  volume       = {22},
  year         = {2017},
}

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